LOCUS       sequence-460478-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gateway compatible Tol2 destination vector containing the fli1a 
            promoter driving Cre and TagBFP in endothelial cells followed by 2 
            cHS4 insulator cores. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(667..678,679..841)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1245..1368
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1409..1714)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1511..1530)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2034..2714)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     2629..2648
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2768..2774,2775..2792,2793..2798))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2823..2946)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2954..2970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2990..3071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3595..3729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3686..3705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3751..3771)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3796..4494)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     intron          5320..5452
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(5687..5707)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5710..5719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8050..8237,8238..8249)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8274..8294)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8274..8292)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8313..8329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8313..8329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8326..8348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8337..8353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8361..8367,8368..8385,8386..8391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8406..8427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8544..8561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8715..9303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8795..8814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9474..10265,10266..10334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10097..10116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10335..10439)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 aattaacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      721 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      781 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      841 aagaaaacta gagattcttg tttaagctat ttaatctcaa aaaacattaa atgaaatgca
      901 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      961 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     1021 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     1081 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     1141 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1201 gatcctaggg ggcccgttta aacgccatga ttacgccaag ctatcaactt tgtatagaaa
     1261 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1321 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
     1381 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
     1441 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
     1501 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     1561 ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca
     1621 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     1681 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca
     1741 aaggagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     1801 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg
     1861 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     1921 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     1981 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     2041 gccggatcct ctagattacg ccccgcctgc cactcatcgc agtactgttg taattcatta
     2101 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     2161 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     2221 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2281 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2341 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2401 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2461 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     2521 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2581 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     2641 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2701 atttttttct ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa
     2761 atccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc
     2821 gccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     2881 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact ttattataca
     2941 tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatca acttgtttat
     3001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3061 tttttcactg cgatatcaat cgggacagcc cccccccaaa gcccccaggg atgtaattac
     3121 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     3181 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     3241 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     3301 tacggggaaa aagctttagg ctgagtgcac ggggacagcc cccccccaaa gcccccaggg
     3361 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     3421 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     3481 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     3541 cctgggggga tacggggaaa aagctttagg ctgatacgta gtagatgcgg ccgcaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3721 gtctggatcg cgatcgcgtc tagattatca tacctttctc ttcttttttg gaccaccagc
     3781 accacctcct cgagcattaa gcttgtgccc cagtttgcta gggaggtcgc agtatctggc
     3841 cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga ttctttccag
     3901 tctgtagtcc acatagtaga cgccaggcat cttgaggttc ttagcgggtt tcttggatct
     3961 atatgtggtc ttggcgtttg cgatcagatg gctcccgccc acgagcttca gggccatgtc
     4021 gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc
     4081 gagtgttttc ttctgcatca cagggccgtt ggatgtgaag ttcacccctc tgatcttgac
     4141 gttgtagatg aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc
     4201 gtcttcgtat gtggtgactc tctcccatgt gaagccctca gggaaggact gcttgaagaa
     4261 gtcggggatg ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga agctagtagc
     4321 caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg
     4381 ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac
     4441 ggtgccctcc atgtacagct tcatgtgcat gttctcctta atcagctcgc tcatggtgga
     4501 tccggccccg ccggctccgc cgctagcgtc tccatcctcc agcagtctca ccatagctcc
     4561 tgtctcagag tccaggtttc tgatgtagtt catcacaatg ttcacatttg tccatcctcc
     4621 agcctgcatg atctcaggga tgctcactcc agctctagcc atgtctctag cagctcccac
     4681 tctagcagag tgtccagacc aagccaggta tctctgtcca gagtcatcct tagctccata
     4741 gatcagtctg tgtgtagcct caaagattcc ctccagagcc cttgtgctca gctggcttgt
     4801 agcagaagga gcagccactc cattctttct cactctacag aacaggtagt tgttagggtc
     4861 atcagccact ccagacacag agatccatct ctccaccagc tttgtcactc ccaggctcag
     4921 agccttctcc actccagctg tgctcaccag tgtctttgtt cttccaatgt ggatcagcat
     4981 tcttcctcca tctgttctgc tgatgtcctt cactctgatt ctagcaatct cagcaattct
     5041 cagcagtgtg ttgtaagcaa ttcccaggaa agccaggttt ctgatgtcct gacatctgtc
     5101 agagttctcc atcaggcttc tcacctggtc aaagtctgtt ctctcaaaag ccagagcctg
     5161 cttagctctc tctccagcat ccacattctc ctttctgatt cttctcatca ccaggctcac
     5221 agcattgctg tcagaaggtc taggcagtcc agaccttctg tgcagcatgt tcagctgtcc
     5281 caggtgctgc tggattgtct tcacagccag tcctctagcc tgtggagaga aaggcaaagt
     5341 ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc tgtctcgaca
     5401 agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt acctggaggt
     5461 acagcaggta gtctctcaca tcctcaggct cagcagggaa ccactttctg ttgttcagct
     5521 tacaccaagc agcccagctc ctgcagacag acagcagcat cttccatgtg tgctcagaga
     5581 aagcctgtct gtctctgaac atgtccatca ggttctttct cacctcatca gatgtagcat
     5641 ccacaggcag agcaggtaga ttttggtgga cggttagaag attggagact ttcctcttct
     5701 tcttgggcac catggtggcc atatgcggca cgcgtcggat ggtttttttt cctctaaatt
     5761 tgggaagtga agtcactgcg cttgagaagg aagccgtgta tgcgtgtatg tgtgtgtgtg
     5821 tatgtgtgtg tgtcactggc cctgccttca ttacggcacc caaagcagga tatttgtcca
     5881 gcgaagataa gacaccaaaa caatggtgtt tctttccttt gtgcatgttt ttgcctggaa
     5941 atatttacag aatgcctgcc aactctttac atttgttgcg ctttttttcc gacattaaac
     6001 aaggcgggtt tgtagaaaca ttgcattgat gcatcaaagc tgtgctagaa ataatcatta
     6061 atacaacgtc tgaaataaca gattatgctg attaatatag gctaaccata gtttaaggct
     6121 aaaataaaat atatactttt tcaacgatcg aatacaaata tgtctaatgc actcggtcta
     6181 ataataatct cataatatga tgaatattgt tccgttatat ccaataactt tgatttgatc
     6241 tacctatggt gaagtgaaga atgtgacgag tctgcccgag cttcttctaa aatgggcttc
     6301 catgaataaa aagcaaagcc cgctgcgtcg agtctgtctg ctccacacgt gtgcgcgcct
     6361 ctgaatgcac atgaacgcag cgagcctggt gctgtatgga ccatatagag aggctgatac
     6421 ctcttgtgtg ctgtgctgtt caacatttct ttaaatcagt agcatatgac ctcacatttt
     6481 tcatggacaa taataggaat attgtgcgtg ttagactaca tttactgtgc attaaatgtc
     6541 tcatttcgtt aaaaaaagaa aaatagatat atttccgact tgtagtgttt agtgtcgaaa
     6601 catattttgc atgcattaaa tgcctgctat ttttatatag catatacagc gtgcatgtga
     6661 ttccatctct aaatccaaaa tctccacccc ctctttttca gttaccgagc acgcattctt
     6721 ggtacattga ggtcgaaatg ctcacaaagc tgctattttc aacatggcta taaattggcc
     6781 gtgtctgcaa ggctcgctta aaccatccag aaccgcagga ttacgctgga atagagattt
     6841 tacgctgagg tgtgtcaaga gctcatcttt gtactgctga atatgctgca tgatgaataa
     6901 tgcatgtatg ctaccgtgct tttaacttaa cagggggaca aaaaactgtt ttcataagcc
     6961 aacttgatcg tgattggctg tcccgcagaa gatgcgtcat gaggtcggga tgtacagaga
     7021 cccattaaaa gcgctgtgct attttataca gagggcattc ctgtcttttg tttcaggagg
     7081 aaaaggaaac ctgacaggaa accagattgt gtggaacagg aaatacccat agattgagac
     7141 acccttctca ttgtcgacta aagtgtacag ttaaaagtca atcatgtgag gtatctgtgt
     7201 caaattgtga aggaaaaaac atgcaggtta ctattataat ctgtggatgc atatgtttac
     7261 tacactgaaa aacccataaa gttatggaaa ctcaaaccgt ttgaggaaac cgactgcaac
     7321 aaaccattta agttcaaaaa ctaatcctaa tgagtactgt gaacttaatc tatttgatta
     7381 aacgatgcaa tttgagcaca tgttgaaccc aataagtgaa gtgaactcaa accaactgag
     7441 tactgtaaaa cccaaaaagt taaggcaact caaactgctt gagggaaccg actgctacaa
     7501 accatttgag ctaaactaat ctatgtgagt accatgaact tactccagtt aagttgaagt
     7561 aatgaggtat ttaatgaact cactaccttc aacactgagt ttaaaactct tttcgaatga
     7621 gtagaatcaa cattcagtaa attttgagtt aattacactc atttcatttg ataaagttga
     7681 ctgttgggtt ttagtgtatg tttatgggtc aagatgagat actagtatcg atgcgaagat
     7741 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     7801 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     7861 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     7921 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     7981 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     8041 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     8101 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     8161 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     8221 taaaattact caagtacttt acacctctga gatctgagct ccagcttttg ttccctttag
     8281 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8341 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8401 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8461 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8521 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8581 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8641 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8701 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8761 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8821 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8881 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8941 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9001 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9061 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9121 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9241 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9301 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9361 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9421 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10441 acatttcccc gaaaagtgcc ac
//
