LOCUS       sequence-460479-        9653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV, tet-off, gateway-compatible destination vector with 
            cre-dependent expression of the insert. EFS driving destablized rTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pAAV-Tet-Off-FLEX-DEST (JDW 1186)
FEATURES             Location/Qualifiers
     source          1..9653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        483..587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(588..656,657..1448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(806..825)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1619..2207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2108..2127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2512..2736
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2803..3336)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3506..3511,3512..3528,3529..3534))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   3874..4003
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        4023..4337
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4031..4049
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4067..4085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4102..4120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4138..4156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4174..4192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4209..4227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4245..4263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4310..4334
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(4362..4395)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4446..4479)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4502..4626
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4667..4972)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4769..4788)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5314..5973)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5888..5907
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6027..6033,6034..6051,6052..6057))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6082..6206)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    6232..6265
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6316..6349
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6394..6982
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6447..6467)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6865..6876)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7015..7032)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7021..7132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7280..7328
                     /note="synthetic polyadenylation signal"
     misc_feature    7342..7433
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7382..7401
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        7473..7684
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(7829..8446,8447..8452,8453..8488,8489..8491,8492..8527,
                     8528..8530,8531..8566,8567..8575)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8588..8604)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8629..8648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8630..8648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8674..8808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   8837..8977
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8837..8966
                     /label=AAV2 ITR
     polyA_signal    9144..9278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  9307..9472
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tcttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
      481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      781 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      841 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      901 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      961 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1021 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1081 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1141 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1201 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1261 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1321 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1381 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1441 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1501 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1741 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2221 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2281 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2341 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     2401 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagaccag ccgcgtaacc
     2461 tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg
     2521 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
     2581 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
     2641 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
     2701 aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa
     2761 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     2821 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     2881 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     2941 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     3001 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     3061 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     3121 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     3181 tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     3241 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     3301 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     3361 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     3421 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     3481 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     3541 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
     3601 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
     3661 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
     3721 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
     3781 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag
     3841 ctatagttct agtggttggc tacagcttgc atgcctgcag gcagctgcgc gctcgctcgc
     3901 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     3961 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgcgtc
     4021 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     4081 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     4141 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     4201 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     4261 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     4321 tgaaccgtca gatcgccacc ggctctagag tcgactccgg aataacttcg tataggatac
     4381 tttatacgaa gttatgcaga atggtagctg gattgtagct gctattagca atatgaaacc
     4441 tcttaataac ttcgtatagc atacattata cgaagttatg gcgcgccgct cgagaggatc
     4501 aaccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     4561 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     4621 actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
     4681 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
     4741 ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
     4801 ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac
     4861 tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca
     4921 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
     4981 cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc
     5041 ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg
     5101 gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt
     5161 cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg
     5221 ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg
     5281 ttatctggct tttagtaagc cggatccaga tctttacgcc ccgccctgcc actcatcgca
     5341 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     5401 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     5461 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     5521 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     5581 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     5641 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     5701 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
     5761 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     5821 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     5881 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     5941 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     6001 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
     6061 tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
     6121 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     6181 cgttcagctt ttttgtacaa acttgttgat ccttgaattc gaatcgctag cataacttcg
     6241 tataaagtat cctatacgaa gttatttgcc ttaacccaga aattatcact gttattcttt
     6301 agaatggtgc aaagaataac ttcgtataat gtatgctata cgaagttatg gcgccagcga
     6361 tccaccggat ctagataact gatccttatc gataatcaac ctctggatta caaaatttgt
     6421 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6481 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6541 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6601 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6661 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6721 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6781 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6841 gggacgccct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6901 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6961 tccctttggg ccgcctcccc gcatcgatac cgtcgactag agctcgctga tcagcctcga
     7021 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7081 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7141 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7201 gggaagacaa tagcagacta gtacgtctct caaggataag taagtaatat taaggtacgg
     7261 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     7321 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     7381 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa
     7441 ctggccgacg cgtggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     7501 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     7561 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     7621 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     7681 acaggggatc cagctcatga gccatggctt cccgccggag gtggaggagc aggatgatgg
     7741 cacgctgccc atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc
     7801 ttctgctagg atcaatgtgg aattcaccat gtctagactg gacaagagca aagtcataaa
     7861 ctctgctctg gaattactca atgaagtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     7921 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     7981 gctcgatgcc ctggcaatcg agatgctgga caggcatcat acccacttct gccccctgga
     8041 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct
     8101 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     8161 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     8221 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg atcaggagca
     8281 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     8341 acaagcaatt gagctgttcg accatcaggg agccgaacct gccttccttt tcggcctgga
     8401 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc
     8461 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     8521 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     8581 taagcatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     8641 tcgtattacg cgcgcagcgg ccgaccatgg cccaacttgt ttattgcagc ttataatggt
     8701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8761 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctc cggacacgtg
     8821 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     8881 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     8941 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggtct gagacaataa ccctgataaa
     9001 tgcttcaata atgtaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     9061 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9121 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9241 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgata tcgcctagga
     9301 gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     9361 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     9421 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
     9481 tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca
     9541 tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta
     9601 atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga atg
//
