LOCUS       sequence-460480-       13671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Tol2 based expression vector for RU486 inducible expression of GFP
            in the nucleus (1st generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 13671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)
FEATURES             Location/Qualifiers
     source          1..13671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4756..4780
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4807..5058
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             6373..6744
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    6874..6995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7565..7611
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    complement(7641..7665)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7673..7693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7739..7741,7742..7744,7745..8458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7739..7741,7742..7744,7745..8455)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(8493..8511)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8494..8511)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8531..8548)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8537..8761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8763..8783)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8791..8807)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8823..8957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(9259..9972,9973..9975,9976..9978))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9262..9972,9973..9975,9976..9978))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(11201..11388,11389..11400)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11434..11450)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11434..11450)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11487..11507)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11487..11505)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11526..11542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11539..11561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11550..11566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11574..11580,11581..11598,11599..11604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11619..11640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11757..11774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11928..12516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12008..12027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12687..13478,13479..13547))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13310..13329
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13548..13652)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt ggctccgaat tcgcccttaa aactcgagac cagcaaagtt
     1261 ctagaatttg tcgaaacatt tatgttatat atttcctgaa aaaaattctg agtaagttct
     1321 taagtgttat tgccagcaac ataaacaaca gacggcaaaa tgaataaatg ataacaaagc
     1381 agtaggctta aataaaccta atttttatag gctgttctct acaaccctca aacagtgatt
     1441 agttttgtac ttataaactt gccctttcat tcatatttca agaaaattgg ttcagaagat
     1501 ctggatattc tagcagttgt tcaagctcat ggagggatca gtgacctgat tccacaatga
     1561 ctaggcctaa tccagaaatt agatgactgt caacataaaa aggcacagca ctcactagct
     1621 gccctatata ttttattata ttttacatat attattttat ttatttagct ctgagtgctg
     1681 tactttctgg ttaaagaaaa ctgcttacaa cagctaacct gtactacctc aggctcaggg
     1741 aatttggaac aggtttgtct ggtttgtttc tttaaccatg catgcttgtt ttcaactatg
     1801 gcaacacagt cacatgggac attacagaaa tgatttgtcg atgacatgcg acttttcttt
     1861 aataaagcgc aaagatccca aaaagcaaac ttttaacaaa aatcatataa ttatattttc
     1921 aatccagctt tgtagcaact ttgtgctgct gttcactcag caacagatag tcagtataag
     1981 gtcagtgtgt ctcaaagcag tgccatctgt ttcacacatt gcgttctata tataagtgtg
     2041 ctggttgaca cgacactgta taaggcctag gctaaaacac aaacaatgta gaatgacact
     2101 gtgttttttt tgtaaacaaa tgttgttttt ggttaaacat ctttgtgaaa acatcctcct
     2161 gtcatgtatt tgctatattc aaatgttaaa cccgtgcaga atagaacata tacaaaaaaa
     2221 aacaacacaa cacattttta aacattatta aatatcaagt attgctggca gttctgtttc
     2281 tgttttacag taccctttgc cacagttctc cgcttttcct ggtccagatt ccacaagtct
     2341 gattcaccaa tagcaaagcg aataaacaac caaagcagcc aatcactgct tgtagactgt
     2401 cctgcgagac cggcccattc cagcacattc tggaaacttc ctttatatga taattataaa
     2461 tacatttaaa ttattgatac aaaacatgta attcctagaa cataaccata gcaatcatta
     2521 gttttcaggg taattatgta tttttaggat ttgactgcgg aaagatctgg tcatgtgacg
     2581 tctcatgaac gtcacggccc tgggtttcta taaatacagt aggactctcg accatcggca
     2641 gatttttcga agaagaagat cagtttcagg agccgtactg ttccgtttca acgcaaatat
     2701 taacggtaag agcgaatttc ctagtttgtt ttcatgccat tctttaaaac catagcgtat
     2761 tactttaatt atagtaaact ttcgctttct ttattacaag agacgttttg tgttgattct
     2821 ccgcggacat tttcggtcag acaatcagaa aatgaccgcg gaggaccagt aacttgcatt
     2881 acacgtaagt taaatcttcg tgtattaaaa tggttaggtt gttaacgtca aataggttac
     2941 cgtgtttgcg tgtgatcagg ttggttttgt tagatttttg tcagtatttt taatttattt
     3001 gttttagttt atttattttt tttgctgaat catagtttgt gaacaaagaa cccggatgtt
     3061 acatacagta cagccgccat gttacagaga gttataactt aatcatttta aaaataattt
     3121 tgccttactt ttagtttgtc atgttgagaa atgaggaaat gttaaaatga ggaaatatcc
     3181 aattaattta atatatcaaa ataatccatg attacaatgc actgaactgg agaaaattaa
     3241 gatgttttct agtgtcatga aacaaatgta agagatgtac attgtagatg ttttatgtca
     3301 agaattggct agttgatgca gcatactggc gatactcagt tgtaataaca gtaacgttac
     3361 atgttaatag actactgagt atgctgttct gtctatgtat gctctgtaag ctacgagaag
     3421 gactttttta aacagtaaag ggtgcaatat ttttacaaat tgaattaaat aaaggctgtc
     3481 tattaagtaa tatgcttgat atttttctta cttgatcgga aataagaaaa aatataaacg
     3541 ttgttgctct aaaaatccta gttcagttta gccaaccaca aatacctttt tgttcctcca
     3601 acagtttttt tttcttctct ataatatttg gcagtctata gtactccaaa tgtttcccca
     3661 cagtctaact aattggtaca gccaaaatca tgacacttat tgcaataata attttggttc
     3721 attggcattg ttgatagcct gtgccactaa tatggtcgat tgatcatgct tcaggaagaa
     3781 aactatattg tttgatgtaa gattattaaa tcttcacctg cttccattac aaactattcc
     3841 catcttattg aattctggta tgtcttaaag gattagttca cttcccaaat caaaatttac
     3901 ttagcctttt ttcatccatg atcccttttt ttcatcatta atgaagaaat tgtttttgaa
     3961 aaagtttcaa gatttttttc tctatattgt ggagcttgtt agtttaaaat tccaaaatgc
     4021 aatatgtggc ttcaaatggt tctaaatgat cccagtcaag gaataacagt cttatctaat
     4081 gaaaccatta gacctttaaa aataaaaaaa ataaaagtat ttatttttaa atgactgagt
     4141 gattaagttg aatttcagcg tttccttact gtgtagaagt ccttccttac tggccccacc
     4201 ctttggttct ctgccaatct gctacctaat gtaatgttgt ggaacattat tattctttat
     4261 ttcttaattt tttatttttt attttaaaac aatgtaaact gcacagatgt gcagtttgtt
     4321 taaaatggcc aatgctttgg aaatgcatga cataattaga tttcatgatg cacaaagcca
     4381 aatctcagag cttgtgcaaa atgagctatc atttcactag gtaagaccct aaattttcat
     4441 ataggatcat ttggacaatt ttgctgcagg taaaatgcat tctatagtcc actgtcagcc
     4501 attgttttgg atagtattta tttttctcta caagtatagt caatagtttt ctattatttt
     4561 aaaggtttgt aacatttaag ggtgaccaaa tgcaaagtaa aatttcattt tcgggtgaac
     4621 tatctcgttt aacatgggag aagtgcaaaa catacattat tggctagaac attgtagtat
     4681 tttttaaatg gaaatgtgtg attgctaatc ttactttgaa tttgtttaca gggatccttt
     4741 aagggcgaat tcgacccaag tttgtacaaa aaagcaggct ggatggcacc caagaagaag
     4801 aggaagatga aagcgttaac ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac
     4861 atcagccaga caggtatgcc gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt
     4921 tccccaaacg cggctgaaga acatctgaag gcgctggcac gcaaaggcgt tattgaaatt
     4981 gtttccggcg catcacgcgg gattcgtctg ttgcaggaag aggaagaagg gttgccgctg
     5041 gtaggtcgtg tggctgccgg tgaaccgtcg agcgccggta ccgaattccc gggtgtcgac
     5101 cagaaaaagt tcaataaagt cagagttgtg agagcactgg atgctgttgc tctcccacag
     5161 ccagtgggcg ttccaaatga aagccaagcc ctaagccaga gattcacttt ttcaccaggt
     5221 caagacatac agttgattcc accactgatc aacctgttaa tgagcattga accagatgtg
     5281 atctatgcag gacatgacaa cacaaaacct gacacctcca gttctttgct gacaagtctt
     5341 aatcaactag gcgagaggca acttctttca gtagtcaagt ggtctaaatc attgccaggt
     5401 tttcgaaact tacatattga tgaccagata actctcattc agtattcttg gatgagctta
     5461 atggtgtttg gtctaggatg gagatcctac aaacacgtca gtgggcagat gctgtatttt
     5521 gcacctgatc taatactaaa tgaacagcgg atgaaagaat catcattcta ttcattatgc
     5581 cttaccatgt ggcagatccc acaggagttt gtcaagcttc aagttagcca agaagagttc
     5641 ctctgtatga aagtattgtt acttcttaat acaattcctt tggaagggct acgaagtcaa
     5701 acccagtttg aggagatgag gtcaagctac attagagagc tcatcaaggc aattggtttg
     5761 aggcaaaaag gagttgtgtc gagctcacag cgtttctatc aacttacaaa acttcttgat
     5821 aacttgcatg atcttgtcaa acaacttcat ctgtactgct tgaatacatt tatccagtcc
     5881 cgggcactga gtgttgaatt tccagaaatg atgtctgaag ttattgctgg gtcgacgccc
     5941 atggaattcc agtacctgcc agatacagac gatcgtcacc ggattgagga gaaacgtaaa
     6001 aggacatatg agaccttcaa gagcatcatg aagaagagtc ctttcagcgg acccaccgac
     6061 ccccggcctc cacctcgacg cattgctgtg ccttcccgca gctcagcttc tgtccccaag
     6121 ccagcacccc agccctatcc ctttacgtca tccctgagca ccatcaacta tgatgagttt
     6181 cccaccatgg tgtttccttc tgggcagatc agccaggcct cggccttggc cccggcccct
     6241 ccccaagtcc tgccccaggc tccagcccct gcccctgctc cagccatggt atcagctctg
     6301 gcccaggccc cagcccctgt cccagtccta gccccaggcc ctcctcaggc tgtggcccca
     6361 cctgccccca agcccaccca ggctggggaa ggaacgctgt cagaggccct gctgcagctg
     6421 cagtttgatg atgaagacct gggggccttg cttggcaaca gcacagaccc agctgtgttc
     6481 acagacctgg catccgtcga caactccgag tttcagcagc tgctgaacca gggcatacct
     6541 gtggcccccc acacaactga gcccatgctg atggagtacc ctgaggctat aactcgccta
     6601 gtgacagggg cccagaggcc ccccgaccca gctcctgctc cactgggggc cccggggctc
     6661 cccaatggcc tcctttcagg agatgaagac ttctcctcca ttgcggacat ggacttctca
     6721 gccctgctga gtcagatcag ctcctaaggc ggccggccgc gactctagat cataatcagc
     6781 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6841 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6901 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6961 agttgtggtt tgtccaaact catcaatgta tcttaagttt aaacgcgtta acaattggaa
     7021 attaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     7081 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     7141 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     7201 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tgatcccccc
     7261 tcgacagctt gcatgccgct tgggctgcag gtcgaggcta aaaaactaat cgcattatca
     7321 tcccctcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
     7381 accactggtt ttatatacag cagtcgacgt actgtacata taaccactgg ttttatatac
     7441 agcagtactg tacatataac cactggtttt atatacagca gtcgaggtaa gattagatat
     7501 ggatatgtat atggatatgt atatggtggt aatgccatgt aatatgctcg actctaggat
     7561 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
     7621 gctagtcgac tctagcctcg acccagcttt cttgtacaaa gtggaaatgg ctccaaagaa
     7681 gaagcgtaag gtaaatcgat cgaattcaag gcctctcgag cctctagaag gtggcggaat
     7741 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     7801 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     7861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     7921 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     7981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     8041 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     8101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     8161 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     8221 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     8281 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     8341 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     8401 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     8461 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
     8521 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8581 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8641 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     8701 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     8761 gccaacttta ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg
     8821 atgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     8881 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     8941 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     9001 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     9061 atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag
     9121 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9181 actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga
     9241 ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
     9301 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
     9361 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     9421 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     9481 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     9541 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     9601 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     9661 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     9721 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     9781 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     9841 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     9901 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     9961 ctcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac
    10021 tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt
    10081 ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc
    10141 tgataactgc acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat
    10201 ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa
    10261 atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta
    10321 cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac
    10381 aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt
    10441 gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga
    10501 catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa
    10561 aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct
    10621 tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt
    10681 gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc
    10741 taaagttaca actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat
    10801 gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca
    10861 caactgattt aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc
    10921 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
    10981 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
    11041 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata
    11101 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg
    11161 caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa
    11221 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa
    11281 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc
    11341 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg
    11401 gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca
    11461 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    11521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    11581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    11641 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11701 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    11761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12361 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca
    12601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    12841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    13021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    13081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    13141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    13201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    13261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    13321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    13381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    13441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    13621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
