LOCUS       sequence-460486-        8498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector containing human BAP1 and Thy1.1 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: BAP1
FEATURES             Location/Qualifiers
     source          1..8498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    443..784
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             851..1267
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1197..1219
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1235..1251
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1316..1340
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2534..2545
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(3537..3561)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3595..4161
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3611..4163
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3755..3772)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3982..4001
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4211..4699
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             4755..5269
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5431..5447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5455..5461,5462..5479,5480..5485))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5500..5521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5638..5655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5809..6397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5889..5908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6568..7359,7360..7428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7191..7210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7429..7533)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7601..7619
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7657..7679)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7779..7798
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7992..8014
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8142..8161
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8361..8498,1..379)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
       61 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      121 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
      181 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
      241 gtcctccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt
      301 gcatccgaat cgtggactcg ctgatccttg ggagggtctc ctcagattga ttgactgccc
      361 acctcggggg tctttcattt ggaggttcca ccgagatttg gagacccctg cccagggacc
      421 accgaccccc ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg tctctgtctt
      481 tgtgcgtgtt tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt tagctaacta
      541 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      601 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      661 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
      721 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
      781 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
      841 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
      901 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
      961 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1021 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1081 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1141 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1201 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1261 aggcgccgga attagatctc tcgagatcga tgcggccgcg tttaaacgtc atcaaacaag
     1321 tttgtacaaa aaagcaggct ccaccatgaa taagggctgg ctggagctgg agagcgaccc
     1381 aggcctcttc accctgctcg tggaagattt cggtgtcaag ggggtgcaag tggaggagat
     1441 ctacgacctt cagagcaaat gtcagggccc tgtatatgga tttatcttcc tgttcaaatg
     1501 gatcgaagag cgccggtccc ggcgaaaggt ctctaccttg gtggatgata cgtccgtgat
     1561 tgatgatgat attgtgaata acatgttctt tgcccaccag ctgataccca actcttgtgc
     1621 aactcatgcc ttgctgagcg tgctcctgaa ctgcagcagc gtggacctgg gacccaccct
     1681 gagtcgcatg aaggacttca ccaagggttt tagccctgag agcaaaggat atgcgattgg
     1741 caatgccccg gagttggcca aggcccataa tagccatgcc aggcccgagc cacgccacct
     1801 ccctgagaag cagaatggcc ttagtgcagt gcggaccatg gaggcgttcc actttgtcag
     1861 ctatgtgcct atcacaggcc ggctctttga gctggatggg ctgaaggtct accccattga
     1921 ccatgggccc tggggggagg acgaggagtg gacagacaag gcccggcggg tcatcatgga
     1981 gcgtatcggc ctcgccactg caggggagcc ctaccacgac atccgcttca acctgatggc
     2041 agtggtgccc gaccgcagga tcaagtatga ggccaggctg catgtgctga aggtgaaccg
     2101 tcagacagta ctagaggctc tgcagcagct gataagagta acacagccag agctgattca
     2161 gacccacaag tctcaagagt cacagctgcc tgaggagtcc aagtcagcca gcaacaagtc
     2221 cccgctggtg ctggaagcaa acagggcccc tgcagcctct gagggcaacc acacagatgg
     2281 tgcagaggag gcggctggtt catgcgcaca agccccatcc cacagccctc ccaacaaacc
     2341 caagctagtg gtgaagcctc caggcagcag cctcaatggg gttcacccca accccactcc
     2401 cattgtccag cggctgccgg cctttctaga caatcacaat tatgccaagt cccccatgca
     2461 ggaggaagaa gacctggcgg caggtgtggg ccgcagccga gttccagtcc gcccacccca
     2521 gcagtactca gatgatgagg atgactatga ggatgacgag gaggatgacg tgcagaacac
     2581 caactctgcc cttaggtata aggggaaggg aacagggaag ccaggggcat tgagcggttc
     2641 tgctgatggg caactgtcag tgctgcagcc caacaccatc aacgtcttgg ctgagaagct
     2701 caaagagtcc cagaaggacc tctcaattcc tctgtccatc aagactagca gcggggctgg
     2761 gagtccggct gtggcagtgc ccacacactc gcagccctca cccaccccca gcaatgagag
     2821 tacagacacg gcctctgaga tcggcagtgc tttcaactcg ccactgcgct cgcctatccg
     2881 ctcagccaac ccgacgcggc cctccagccc tgtcacctcc cacatctcca aggtgctttt
     2941 tggagaggat gacagcctgc tgcgtgttga ctgcatacgc tacaaccgtg ctgtccgtga
     3001 tctgggtcct gtcatcagca caggcctgct gcacctggct gaggatgggg tgctgagtcc
     3061 cctggcgctg acagagggtg ggaagggttc ctcgccctcc atcagaccaa tccaaggcag
     3121 ccaggggtcc agcagcccag tggagaagga ggtcgtggaa gccacggaca gcagagagaa
     3181 gacggggatg gtgaggcctg gcgagccctt gagtggggag aaatactcac ccaaggagct
     3241 gctggcactg ctgaagtgtg tggaggctga gattgcaaac tatgaggcgt gcctcaagga
     3301 ggaggtagag aagaggaaga agttcaagat tgatgaccag agaaggaccc acaactacga
     3361 tgagttcatc tgcaccttta tctccatgct ggctcaggaa ggcatgctgg ccaacctagt
     3421 ggagcagaac atctccgtgc ggcggcgcca aggggtcagc atcggccggc tccacaagca
     3481 gcggaagcct gaccggcgga aacgctctcg cccctacaag gccaagcgcc agtaggaccc
     3541 agctttcttg tacaaagtgg ttcgatgacg gtatcgataa gcttgatatc gaattccgcc
     3601 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3661 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3721 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3781 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3841 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3901 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3961 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     4021 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     4081 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4141 ctttgaaaaa cacgatgata atatggccac aaccatgggt cttttctgca gtcaccgtcc
     4201 tcgaggcacc atgaacccag ccatcagcgt cgctctcctg ctctcagtct tgcaggtgtc
     4261 ccgagggcag aaggtgacca gcctgacagc ctgcctggtg aaccaaaacc ttcgcctgga
     4321 ctgccgccat gagaataaca ccaaggataa ctccatccag catgagttca gcctgacccg
     4381 agagaagagg aagcacgtgc tctcaggcac ccttgggata cccgagcaca cgtaccgctc
     4441 ccgcgtcacc ctctccaacc agccctatat caaggtcctt accctagcca acttcaccac
     4501 caaggatgag ggcgactact tttgtgagct tcgcgtctcg ggcgcgaatc ccatgagctc
     4561 caataaaagt atcagtgtgt atagagacaa gctggtcaag tgtggcggca taagcctgct
     4621 ggttcagaac acatcctgga tgctgctgct gctgctttcc ctctccctcc tccaagccct
     4681 ggacttcatt tctctgtgac gcgtagaatt cgataaaata aaagatttta tttagtctcc
     4741 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     4801 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     4861 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     4921 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     4981 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     5041 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     5101 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     5161 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     5221 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt
     5281 tcttgaagtt ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc
     5341 aattagccac tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca
     5401 gcgcagaaag agctggggag aattgtgaaa ttgttatccg ctcacaattc cacacaacat
     5461 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5521 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5581 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5641 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5701 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5761 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5821 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5881 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5941 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6001 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6061 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6121 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6181 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6241 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6421 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6481 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6541 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6601 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6661 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6721 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6781 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6841 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6901 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6961 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7021 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7081 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7141 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7201 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7261 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7321 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7381 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7441 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7501 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7561 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     7621 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7681 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7741 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     7801 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7861 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     7921 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     7981 gccagggttt tcccagtcac gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag
     8041 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     8101 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     8161 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     8221 cgattagtcc aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa
     8281 caatatcacc agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt
     8341 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     8401 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     8461 ggttaggaac agagagacag cagaatatgg gccaaaca
//
