LOCUS       sequence-460505-        8798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the
            periplasmic signal. His-tag for purification. TEV enzyme recognition
            site between Ag43 alpha subunit and the fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Kirpat Konak BM, Bakar ME, Ahan RE, Ozyurek EU, Dokmeci S, Safak 
            Seker UO
  TITLE     A living material platform for the biomineralization of biosilica.
  JOURNAL   Mater Today Bio. 2022 Oct 10;17:100461. doi: 
            10.1016/j.mtbio.2022.100461. eCollection 2022 Dec 15.
  PUBMED    36278145
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mater Today
            Bio."; date: "2022-10-10"; volume: "17:100461. doi"; pages: " 
            10.1016/j.mtbio.2022.100461. eCollection 2022 Dec 15"
COMMENT     Sequence Label: pET22b Ag43 160N 6H sfGFP-R5 AmpR
FEATURES             Location/Qualifiers
     source          1..8798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2672..2692)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(2708..3421)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(3509..3526)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     sig_peptide     complement(3533..3598)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     RBS             3606..3628
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    3643..3667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3667..3686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3668..3686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3770..3789)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3995..4072
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4073..5155
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4092..4111)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             5964..6155
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6149..6171)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6257..6396
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6312..6331
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6411..6428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6582..7170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6662..6681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7341..8132,8133..8201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7964..7983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8202..8306)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8332..8787)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8469..8490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8681..8700
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtcatcag aaggtcacat
      181 tcagtgtggc ctgaccgtta tacccttcag cgctgctgcc gctgacgctg tgggcataac
      241 cggcctgaac gcccagggtg atattttccc ggacacgggc ttccagtccg gcctgcaggt
      301 ccagtgatgt gccattctgt gagggagaga acgtcatccc gctgcctgca gtggaagtcc
      361 ccacacgcat atctccccgg gagctgaagg tgcggataac agaaggctgt acccaccagt
      421 tcaccggtaa ttcactcaca ctgtgttttg cactgtcacg caggggggca cgggatgagg
      481 tgccttcgcc aaaggtcata tcgttgtggc tgcccagacg gaaaccggca cgcacatgtt
      541 gtgcactgcc atgcccgaac ttcacataac cggcgttgtc cttaccgtca tccagggaaa
      601 gtccctgcca ggtatactgc agttgtggct ccagcatcag gttgtcagtg atactgaagg
      661 gcagaccggt ttccagtgag cccagccagc cccagccccg ggcgcggaag tcgttattgt
      721 ccgatgacgc tttcatgctg tggcgggttc cctgtgccac aatgtcagcc cacaggccgg
      781 aggacgtgtg taccagattc aggtatccgc ccaggctgcc ggcatcatcc cggaccgtgc
      841 cggcacggga gccgtcatca tccttaacat caacggaaga atggccagca gcaccatata
      901 cccccgcggt cacagacata ccggcaacct ctgttctcat caggtcaccc tccagacgga
      961 cgaatccata gctgccgctg ctttccggcg tggccccacg ggcaataccg ccattgttat
     1021 cgtgaccgag atgaccgccc tgaatgctga gacggacgct gttgttttca ccatttacac
     1081 cggtctgatg gctgcgggag cctgccacaa tccggtcata gtccattgcc tgtgtcagca
     1141 tggaggcata cagggggact tctgcacgat aagcattttc actgcgcaga taccagctct
     1201 catcactgtc ccggttgagg gagtagttaa aggcaccggc ctgcagcctg ttcccctgga
     1261 caaaggcccc ttcctccgtg gtggcaccgt taatggcttc caccacctga atacccttac
     1321 cgctggtcgc cagccccgac gcactgttgc cggcgttcac caggttcagg atggtttttc
     1381 cggttgccct gccgccgtca atgaccagtc tgtcagcatt gttctgtgcc atatccgggc
     1441 gtacacgcag gctgatggtg ccattctgtc cgttcaggtt tttcactttc agggttgccg
     1501 gtacgaactt ccctgtgcgg gtggaggtga aatgaatctg tccggcatgg ctgaggtcat
     1561 ccaccaccga ctgcaccgtg gcgttatcgg ggatattcca ggtggcaccg gaggcgagag
     1621 tgacattcgt ggggtcaatg gcaccgttca gcacagtgct gccggtcagc ttcagggctg
     1681 taccgcgctg agcaatgaca tccgtggtga cggtactgtc gttcagcgtc agcgtgcctt
     1741 cattcaggtt gacggctttc tgggtgagtg tggtgttgct gacagtgagt gtgccacttc
     1801 ctgatttttc cacgctgccg ttaccggtga gagagcctcc ctgcaggagt gcatcgcctt
     1861 cacggatggt cagggtatcg ttgttcaccg tcttcccgga aagggtaagt atggcgccgt
     1921 tattcagcgt ggtggtgccc gccagtgtgc cgcccctggc ggtgaacagt ccgccattta
     1981 ccgtggtatc cgtggccgta tcaccggcgt tcagcgtgaa tgaactgcct ttttccagca
     2041 tcagggcatc cgcctgaccg cctccgatac tgaatgcctt tccgtcgctc cgggtaccac
     2101 tgacagcggc accggaatcg gccagcagta caccgccatt tcccatggat acggtggtgg
     2161 cggtgccacc gttgcggaca tccagcgttc cgccatcatc cacgcgggta ttagtggctg
     2221 tgtgtccggt cagcacatcc aggcgtccgc cattttccag tacgacatta tcagctttac
     2281 cctccacaac agagaatgct cccaggcggt ttatgccggt aacggttgca gccgtactgg
     2341 taaccagtgc gccgccctgc ttcagggtga cattcgtggc tgtaccaccg gcgtccacct
     2401 gcagtctgcc cttctgatta acggtggtat tcccggccac acccccgttt ttgacaatct
     2461 gccagccgtc actgttcaca acagtgtcag acgctgtacc gccgttgtgc acatactggt
     2521 atcccccatt cagcgtggta tccagtgcgt gaccatgtac agtctggtcg ccgccggcat
     2581 aaaccacagt ggtatttgcc gttccttcag ctctcacaat ctggcgaccg tttttattga
     2641 tggttgtgcg cttaaggcta ccaccgccac cgccctgaaa gtacaggttt tcgctaccac
     2701 cgccaccttt gtacagttca tccataccat gcgtgatgcc cgctgcggtt acgaactcca
     2761 gcagaaccat atgatcgcgt ttctcgttcg gatctttaga cagaacgctt tgcgtgctca
     2821 gatagtgatt gtctggcagc agaacaggac catcaccgat tggagtgttt tgctggtagt
     2881 gatcagccag ctgcacgctg ccatcctcca cgttgtggcg aattttaaaa ttcgctttaa
     2941 tgccattttt ttgtttatcg gcggtgatgt aaacattgtg gctgttaaaa ttgtattcca
     3001 gcttatggcc caggatattg ccgtcttctt taaagtcaat gcctttcagc tcaatgcggt
     3061 ttaccagggt atcgccttca aatttcactt ccgcacgcgt tttgtacgtg ccgtcatcct
     3121 taaaggaaat cgtgcgttcc tgcacatagc cttccggcat ggcggacttg aagaagtcat
     3181 gctgcttcat atggtccgga taacgagcaa agcactgaac accataagtc agcgtcgtta
     3241 ccagagtcgg ccaaggtacc ggcagtttac cagtagtaca gatgaacttc agcgtcagtt
     3301 taccattagt tgcgtcacct tcaccctcgc cacgcacgga aaacttatga ccgttgacat
     3361 caccatccag ttccaccaga atagggacga caccagtgaa cagctcttcg cctttacgca
     3421 tgctgccacc gccacccaga atgcggcgtt tgctgccttt gctgccgcta tagctgccgc
     3481 tttttttgct gctggatcca ccgccaccgt ggtggtggtg gtggtggccc atggccatcg
     3541 ccggctgggc agcgaggagc agcagaccag cagcagcggt cggcagcagg tatttcatat
     3601 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
     3661 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
     3721 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     3781 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     3841 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     3901 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     3961 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
     4021 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     4081 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     4141 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     4201 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
     4261 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     4321 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     4381 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     4441 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     4501 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     4561 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     4621 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     4681 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     4741 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     4801 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     4861 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     4921 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     4981 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     5041 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5101 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     5161 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
     5221 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     5281 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     5341 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     5401 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     5461 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc
     5521 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     5581 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     5641 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     5701 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     5761 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     5821 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     5881 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     5941 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     6001 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     6061 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     6121 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6181 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6241 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     6301 gcttaactat gcggcatcag agcagattgt actgagagtg caccattgcg gtgtgaaata
     6361 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     6421 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6481 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6541 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6601 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6661 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6721 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6781 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6841 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6901 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6961 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7021 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7081 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7141 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7201 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7261 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7321 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7381 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7441 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7501 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7561 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7621 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     7681 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7741 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7801 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7861 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7921 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7981 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8041 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8101 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8161 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8221 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8281 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     8341 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8401 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8461 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8521 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8581 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8641 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8701 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8761 cgcttaatgc gccgctacag ggcgcgtccc attcgcca
//
