LOCUS       sequence-460510-        4876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mScarletI3 with URA3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-ymScarletI3-URA3
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(568..672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     740..758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(796..818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     918..937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1018..1036
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1018..1035
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1819..2006
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1874..1898)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2081..2424
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3243..3440
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3547..3566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3548..3566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3653..3670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3824..4412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3904..3923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4583..4876,1..498,499..567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
       61 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
      181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
      241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
      301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
      361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
      421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
      481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
      541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
      601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      781 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      841 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      901 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      961 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
     1021 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
     1081 cggtgacggt gctggtttaa ttaacatgga ttctactgaa gctgttatta aggaattcat
     1141 gagatttaag gttcatatgg aaggttctat gaatggtcat gaatttgaaa ttgaaggtga
     1201 aggtgaaggt agaccatatg aaggtactca aactgctaag ttgaaggtca ctaagggtgg
     1261 tccattgcca ttctcttggg atattttgtc tccacaattc atgtacggtt ctagagcttt
     1321 tattaagcat ccagctgaca ttccagatta ctggaagcaa tctttcccag aaggttttaa
     1381 atgggaaaga gttatgatct ttgaagatgg tggtactgtt tctgttactc aagatacttc
     1441 tttggaagat ggtactttga tttacaaggt taagttgaga ggtggtaact tcccaccaga
     1501 tggtccagtt atgcaaaaga gaactatggg ttgggaagcc tctactgaaa gattgtaccc
     1561 agaagatgtt gttttaaagg gtgatattaa gatggctttg agattgaagg atggtggtag
     1621 atacttggct gattttaaga ctacttataa ggctaagaag ccagttcaaa tgccaggtgc
     1681 tttcaacatt gatagaaagt tggatattac ttctcataac gaagattaca ctgttgttga
     1741 acaatacgaa agatctgttg ctagacattc tactggtggt tctggtggtt cttaagcggc
     1801 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     1861 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     1921 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
     1981 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc
     2041 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     2101 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     2161 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     2221 gcgaagcaaa aattacggct cctcgctgcg gacctgcgag cagggaaacg ctcccctcac
     2281 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     2341 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     2401 catcacatcc gaacataaac aaccatgaca gtcaacacta agacctatag tgagagagca
     2461 gaaactcatg cctcaccagt agcacaacga ttatttcgat taatggaact gaagaaaacc
     2521 aatttatgtg catcaattga tgttgatacc actaaggaat tccttgaatt aattgataaa
     2581 ttgggtcctt atgtatgctt aatcaagact catattgata taatcaatga tttttcctat
     2641 gaatccacta ttgaaccatt attagaactt tcacgtaaac atcaatttat gatttttgaa
     2701 gatagaaaat ttgctgatat tggtaatacc gtgaagaaac aatatattgg tggagtttat
     2761 aaaattagta gttgggcaga tattactaat gctcatggtg tcactgggaa tggagtagtt
     2821 gaaggattaa aacagggagc taaagaaacc accaccaacc aagagccaag agggttattg
     2881 atgttagctg aattatcatc agtgggatca ttagcatatg gagaatattc tcaaaaaact
     2941 gttgaaattg ctaaatccga taaggaattt gttattggat ttattgccca acgtgatatg
     3001 ggtggacaag aagaaggatt tgattggctt attatgacac ctggagttgg attagatgat
     3061 aaaggtgatg gattaggaca acaatataga actgttgatg aagttgttag cactggaact
     3121 gatattatca ttgttggtag aggattgttt ggtaaaggaa gagatccaga tattgaaggt
     3181 aaaaggtata gagatgctgg ttggaatgct tatttgaaaa agactggcca attataatca
     3241 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     3301 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     3361 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     3421 gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa
     3481 cgagctcgaa ttcatcgatg atatcagatc cactagtggc ctatgcggcc gcggatctgc
     3541 cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa
     3601 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3961 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4021 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4081 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4141 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4201 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4261 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4321 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4381 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4441 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4501 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4561 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4621 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4681 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4741 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4801 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4861 cgccagttaa tagttt
//
