LOCUS       sequence-460513-        4722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mScarletI3 with SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4722)
  TITLE     Teusink Lab yeast expression plasmids
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-ymScarletI3-SpHis5
FEATURES             Location/Qualifiers
     source          1..4722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     329..348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(567..671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     739..757
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(795..817)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     917..936
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1017..1035
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1017..1034
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1826..2013
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1881..1905)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            2088..3288
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        2088..2431
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2432..3085
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      3091..3288
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3392..3411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3393..3411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3498..3515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3669..4257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3749..3768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4428..4722,1..497,498..566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
       61 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
      121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
      181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
      241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
      301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
      361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
      421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
      481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
      541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
      601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
      661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
      721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
      781 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
      841 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      901 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatggacata
      961 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
     1021 aggtgacact atagaacgcg gccgccagct gaagcttcgt acgctgcagg tcgacggatc
     1081 ggtgacggtg ctggtttaat taacatggat tctactgaag ctgttattaa ggaattcatg
     1141 agatttaagg ttcatatgga aggttctatg aatggtcatg aatttgaaat tgaaggtgaa
     1201 ggtgaaggta gaccatatga aggtactcaa actgctaagt tgaaggtcac taagggtggt
     1261 ccattgccat tctcttggga tattttgtct ccacaattca tgtacggttc tagagctttt
     1321 attaagcatc cagctgacat tccagattac tggaagcaat ctttcccaga aggttttaaa
     1381 tgggaaagag ttatgatctt tgaagatggt ggtactgttt ctgttactca agatacttct
     1441 ttggaagatg gtactttgat ttacaaggtt aagttgagag gtggtaactt cccaccagat
     1501 ggtccagtta tgcaaaagag aactatgggt tgggaagcct ctactgaaag attgtaccca
     1561 gaagatgttg ttttaaaggg tgatattaag atggctttga gattgaagga tggtggtaga
     1621 tacttggctg attttaagac tacttataag gctaagaagc cagttcaaat gccaggtgct
     1681 ttcaacattg atagaaagtt ggatattact tctcataacg aagattacac tgttgttgaa
     1741 caatacgaaa gatctgttgc tagacattct actggtggtt ctggtggttc ttaagcggcc
     1801 gcctcgaggt cccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     1861 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     1921 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta
     1981 tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata acagggtaat
     2041 atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
     2101 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
     2161 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
     2221 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
     2281 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
     2341 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
     2401 gttctcacat cacatccgaa cataaacaac catgggtagg agggcttttg tagaaagaaa
     2461 tacgaacgaa acgaaaatca gcgttgccat cgctttggac aaagctccct tacctgaaga
     2521 gtcgaatttt attgatgaac ttataacttc caagcataca aaccaaaagg gagaacaagt
     2581 aatccaagta gacacgggaa ttggattctt ggatcacatg tatcatgcac tggctaaaca
     2641 tgcaggctgg agcttacgac tttactcaag aggtgattta atcatcgatg atcatcacac
     2701 tgcagaagat actgctattg cacttggtat tgcattcaag caggctatga gtaactttgc
     2761 cggcgttaaa agatttggac atgcttattg tccacttgac gaagctcttt ctagaagcgt
     2821 agttgacttg tcgggacggc cctatgctgt tatcgatttg ggattaaagc gtgaaaaggt
     2881 tggggaattg tcctgtgaaa tgatccctca cttactatat tccttttcgg tagcagctgg
     2941 aattactttg catgttacct gcttatatgg tagtaatgac catcatcgtg ctgaaagcgc
     3001 ttttaaatct ctggctgttg ccatgcgcgc ggctactagt cttactggaa gttctgaagt
     3061 cccaagcacg aagggagtgt tgtaaagagt actgacaata aaaagattct tgttttcaag
     3121 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
     3181 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
     3241 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     3301 tactaacgcc gccatccagt ttaaacgagc tcgaattcat cgatgatatc agatccacta
     3361 gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata
     3421 agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3481 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3541 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3601 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3661 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3721 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3781 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3841 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3901 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3961 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4021 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4081 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4141 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4201 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4261 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4321 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4381 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4441 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4501 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4561 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4621 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4681 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tg
//
