LOCUS       sequence-460517-       13855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Tol2 based expression vector for RU486 inducible expression of GFP
            in the nucleus with a destablized lex transactivator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)
FEATURES             Location/Qualifiers
     source          1..13855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(618..629,630..792)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4756..4780
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4799..4808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4829..5080
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             6395..6766
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    7035..7156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7726..7772
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    complement(7825..7849)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7857..7877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7923..7925,7926..7928,7929..8642)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7923..7925,7926..7928,7929..8639)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(8677..8695)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8678..8695)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8715..8732)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8721..8945
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8947..8967)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8975..8991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    9007..9141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(9443..10156,10157..10159,10160..10162))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9446..10156,10157..10159,10160..10162))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(11385..11572,11573..11584)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11618..11634)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11618..11634)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11671..11691)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11671..11689)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11710..11726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11723..11745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11734..11750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11758..11764,11765..11782,11783..11788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11803..11824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11941..11958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12112..12700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12192..12211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12871..13662,13663..13731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13494..13513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13732..13836)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt ggctccgaat tcgcccttaa aactcgagac cagcaaagtt
     1261 ctagaatttg tcgaaacatt tatgttatat atttcctgaa aaaaattctg agtaagttct
     1321 taagtgttat tgccagcaac ataaacaaca gacggcaaaa tgaataaatg ataacaaagc
     1381 agtaggctta aataaaccta atttttatag gctgttctct acaaccctca aacagtgatt
     1441 agttttgtac ttataaactt gccctttcat tcatatttca agaaaattgg ttcagaagat
     1501 ctggatattc tagcagttgt tcaagctcat ggagggatca gtgacctgat tccacaatga
     1561 ctaggcctaa tccagaaatt agatgactgt caacataaaa aggcacagca ctcactagct
     1621 gccctatata ttttattata ttttacatat attattttat ttatttagct ctgagtgctg
     1681 tactttctgg ttaaagaaaa ctgcttacaa cagctaacct gtactacctc aggctcaggg
     1741 aatttggaac aggtttgtct ggtttgtttc tttaaccatg catgcttgtt ttcaactatg
     1801 gcaacacagt cacatgggac attacagaaa tgatttgtcg atgacatgcg acttttcttt
     1861 aataaagcgc aaagatccca aaaagcaaac ttttaacaaa aatcatataa ttatattttc
     1921 aatccagctt tgtagcaact ttgtgctgct gttcactcag caacagatag tcagtataag
     1981 gtcagtgtgt ctcaaagcag tgccatctgt ttcacacatt gcgttctata tataagtgtg
     2041 ctggttgaca cgacactgta taaggcctag gctaaaacac aaacaatgta gaatgacact
     2101 gtgttttttt tgtaaacaaa tgttgttttt ggttaaacat ctttgtgaaa acatcctcct
     2161 gtcatgtatt tgctatattc aaatgttaaa cccgtgcaga atagaacata tacaaaaaaa
     2221 aacaacacaa cacattttta aacattatta aatatcaagt attgctggca gttctgtttc
     2281 tgttttacag taccctttgc cacagttctc cgcttttcct ggtccagatt ccacaagtct
     2341 gattcaccaa tagcaaagcg aataaacaac caaagcagcc aatcactgct tgtagactgt
     2401 cctgcgagac cggcccattc cagcacattc tggaaacttc ctttatatga taattataaa
     2461 tacatttaaa ttattgatac aaaacatgta attcctagaa cataaccata gcaatcatta
     2521 gttttcaggg taattatgta tttttaggat ttgactgcgg aaagatctgg tcatgtgacg
     2581 tctcatgaac gtcacggccc tgggtttcta taaatacagt aggactctcg accatcggca
     2641 gatttttcga agaagaagat cagtttcagg agccgtactg ttccgtttca acgcaaatat
     2701 taacggtaag agcgaatttc ctagtttgtt ttcatgccat tctttaaaac catagcgtat
     2761 tactttaatt atagtaaact ttcgctttct ttattacaag agacgttttg tgttgattct
     2821 ccgcggacat tttcggtcag acaatcagaa aatgaccgcg gaggaccagt aacttgcatt
     2881 acacgtaagt taaatcttcg tgtattaaaa tggttaggtt gttaacgtca aataggttac
     2941 cgtgtttgcg tgtgatcagg ttggttttgt tagatttttg tcagtatttt taatttattt
     3001 gttttagttt atttattttt tttgctgaat catagtttgt gaacaaagaa cccggatgtt
     3061 acatacagta cagccgccat gttacagaga gttataactt aatcatttta aaaataattt
     3121 tgccttactt ttagtttgtc atgttgagaa atgaggaaat gttaaaatga ggaaatatcc
     3181 aattaattta atatatcaaa ataatccatg attacaatgc actgaactgg agaaaattaa
     3241 gatgttttct agtgtcatga aacaaatgta agagatgtac attgtagatg ttttatgtca
     3301 agaattggct agttgatgca gcatactggc gatactcagt tgtaataaca gtaacgttac
     3361 atgttaatag actactgagt atgctgttct gtctatgtat gctctgtaag ctacgagaag
     3421 gactttttta aacagtaaag ggtgcaatat ttttacaaat tgaattaaat aaaggctgtc
     3481 tattaagtaa tatgcttgat atttttctta cttgatcgga aataagaaaa aatataaacg
     3541 ttgttgctct aaaaatccta gttcagttta gccaaccaca aatacctttt tgttcctcca
     3601 acagtttttt tttcttctct ataatatttg gcagtctata gtactccaaa tgtttcccca
     3661 cagtctaact aattggtaca gccaaaatca tgacacttat tgcaataata attttggttc
     3721 attggcattg ttgatagcct gtgccactaa tatggtcgat tgatcatgct tcaggaagaa
     3781 aactatattg tttgatgtaa gattattaaa tcttcacctg cttccattac aaactattcc
     3841 catcttattg aattctggta tgtcttaaag gattagttca cttcccaaat caaaatttac
     3901 ttagcctttt ttcatccatg atcccttttt ttcatcatta atgaagaaat tgtttttgaa
     3961 aaagtttcaa gatttttttc tctatattgt ggagcttgtt agtttaaaat tccaaaatgc
     4021 aatatgtggc ttcaaatggt tctaaatgat cccagtcaag gaataacagt cttatctaat
     4081 gaaaccatta gacctttaaa aataaaaaaa ataaaagtat ttatttttaa atgactgagt
     4141 gattaagttg aatttcagcg tttccttact gtgtagaagt ccttccttac tggccccacc
     4201 ctttggttct ctgccaatct gctacctaat gtaatgttgt ggaacattat tattctttat
     4261 ttcttaattt tttatttttt attttaaaac aatgtaaact gcacagatgt gcagtttgtt
     4321 taaaatggcc aatgctttgg aaatgcatga cataattaga tttcatgatg cacaaagcca
     4381 aatctcagag cttgtgcaaa atgagctatc atttcactag gtaagaccct aaattttcat
     4441 ataggatcat ttggacaatt ttgctgcagg taaaatgcat tctatagtcc actgtcagcc
     4501 attgttttgg atagtattta tttttctcta caagtatagt caatagtttt ctattatttt
     4561 aaaggtttgt aacatttaag ggtgaccaaa tgcaaagtaa aatttcattt tcgggtgaac
     4621 tatctcgttt aacatgggag aagtgcaaaa catacattat tggctagaac attgtagtat
     4681 tttttaaatg gaaatgtgtg attgctaatc ttactttgaa tttgtttaca gggatccttt
     4741 aagggcgaat tcgacccaag tttgtacaaa aaagcaggct tacgtaacta gtggatccgc
     4801 caccatggca cccaagaaga agaggaagat gaaagcgtta acggccaggc aacaagaggt
     4861 gtttgatctc atccgtgatc acatcagcca gacaggtatg ccgccgacgc gtgcggaaat
     4921 cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa gaacatctga aggcgctggc
     4981 acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga
     5041 agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccgt cgagcgccgg
     5101 taccgaattc ccgggtgtcg accagaaaaa gttcaataaa gtcagagttg tgagagcact
     5161 ggatgctgtt gctctcccac agccagtggg cgttccaaat gaaagccaag ccctaagcca
     5221 gagattcact ttttcaccag gtcaagacat acagttgatt ccaccactga tcaacctgtt
     5281 aatgagcatt gaaccagatg tgatctatgc aggacatgac aacacaaaac ctgacacctc
     5341 cagttctttg ctgacaagtc ttaatcaact aggcgagagg caacttcttt cagtagtcaa
     5401 gtggtctaaa tcattgccag gttttcgaaa cttacatatt gatgaccaga taactctcat
     5461 tcagtattct tggatgagct taatggtgtt tggtctagga tggagatcct acaaacacgt
     5521 cagtgggcag atgctgtatt ttgcacctga tctaatacta aatgaacagc ggatgaaaga
     5581 atcatcattc tattcattat gccttaccat gtggcagatc ccacaggagt ttgtcaagct
     5641 tcaagttagc caagaagagt tcctctgtat gaaagtattg ttacttctta atacaattcc
     5701 tttggaaggg ctacgaagtc aaacccagtt tgaggagatg aggtcaagct acattagaga
     5761 gctcatcaag gcaattggtt tgaggcaaaa aggagttgtg tcgagctcac agcgtttcta
     5821 tcaacttaca aaacttcttg ataacttgca tgatcttgtc aaacaacttc atctgtactg
     5881 cttgaataca tttatccagt cccgggcact gagtgttgaa tttccagaaa tgatgtctga
     5941 agttattgct gggtcgacgc ccatggaatt tcagtacctg ccagatacag acgatcgtca
     6001 ccggattgag gagaaacgta aaaggacata tgagaccttc aagagcatca tgaagaagag
     6061 tcctttcagc ggacccaccg acccccggcc tccacctcga cgcattgctg tgccttcccg
     6121 cagctcagct tctgtcccca agccagcacc ccagccctat ccctttacgt catccctgag
     6181 caccatcaac tatgatgagt ttcccaccat ggtgtttcct tctgggcaga tcagccaggc
     6241 ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc ctgcccctgc
     6301 tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc tagccccagg
     6361 ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg aaggaacgct
     6421 gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct tgcttggcaa
     6481 cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg agtttcagca
     6541 gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc tgatggagta
     6601 ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc cagctcctgc
     6661 tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag acttctcctc
     6721 cattgcggac atggacttct cagccctgct gagtcagatc agctccagat ctcgagatat
     6781 cagccatggc ttcccgccgg cggtggcggc gcaggatgat ggcacgctgc ccatgtcttg
     6841 tgcccaggag agcgggatgg accgtcaccc tgcagcctgt gcttctgcta ggatcaatgt
     6901 gtagtaaatc gatgatcctc cacgtagcgg ccgcaatcag ccataccaca tttgtagagg
     6961 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     7021 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7141 tcatcaatgt atcttaagtt taaacgcgtt aacaattgga aattaagatt gaatcctgtt
     7201 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     7261 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     7321 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     7381 gcggtgtcat ctatgttact agatcgggaa ttgatccccc ctcgacagct tgcatgccgc
     7441 ttgggctgca ggtcgaggct aaaaaactaa tcgcattatc atcccctcga cgtactgtac
     7501 atataaccac tggttttata tacagcagta ctgtacatat aaccactggt tttatataca
     7561 gcagtcgacg tactgtacat ataaccactg gttttatata cagcagtact gtacatataa
     7621 ccactggttt tatatacagc agtcgaggta agattagata tggatatgta tatggatatg
     7681 tatatggtgg taatgccatg taatatgctc gactctagga tcttcgcaag acccttcctc
     7741 tatataagga agttcatttc atttggagag gacacgctga agctagtcga ctctagcctc
     7801 ggctagcaac tctagattaa ttaaacccag ctttcttgta caaagtggaa atggctccaa
     7861 agaagaagcg taaggtaaat cgatcgaatt caaggcctct cgagcctcta gaaggtggcg
     7921 gaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     7981 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     8041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     8101 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     8161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     8221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     8281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     8341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     8401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     8461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     8521 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     8581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     8641 aagcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag
     8701 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     8761 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     8821 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8881 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     8941 tatggccaac tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc
     9001 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     9061 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     9121 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     9181 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     9241 tatgatcctc tagatcagat ctcttgttta ttgcagctta taatggttac aaataaagca
     9301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9361 ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc
     9421 tagaggctcg agaggggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
     9481 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     9541 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     9601 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     9661 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     9721 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     9781 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     9841 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     9901 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     9961 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
    10021 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
    10081 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
    10141 gctcctcgcc cttgctcacc atggtcactg tctgctttgc tgttggtctg ggctcctggg
    10201 tcactggctt actaatggag tctttatgta tgaggactct tatcaattgt tcttctataa
    10261 aggtctgcag tgtttctgtt cgtcccctac atggacaccc agagcctcct aaatacagga
    10321 gccctgataa ctgcacaagt gctcagattc cagcagggtg gaaaatgaga taaagtgtgc
    10381 agatggggag ggggacgtga atgagagatt tgagggatga aaaggatgga tgaacgcatt
    10441 gaaaatagcc cctttcacaa aataatacca gtaaattgtc atataattaa tgaccagacc
    10501 tttacctgta aatgatgtga tgttcatata cagaagaatg ccaacaattt acagatgatt
    10561 ttacaaattc ttaattttcc ttttctaaat ggatttgttc acacattatc tcttcaaagc
    10621 aattgaattt tctggaaaag attggctgtg tgtgtaaaag gtgctactga ggaatttaat
    10681 gtgacatgga atgaagcaaa cagcagccta agacaggatg gcaggaaaaa tgtcacatgt
    10741 ttaaaataag agtgaaacca acgacctgga cttatagaga ctgtgtgtcc ttaggcaaag
    10801 ctcttgtaca aacagtgctt gtgactgtga aattcggggt ttgcctggat tgtgttaaat
    10861 gtgtgcttgg atgtatcata aaaaagaatc ggttctctgt atttgacatt ttctgtaatt
    10921 tatctaaagt tacaactggc tatgcctgat ttttatcact attgaatcaa tcattttaaa
    10981 gaatgttaag tctgctgatt tggagagctc atttacattt attttgaatg tctcttattt
    11041 aacacaactg atttaagctt tagatctgcg aagatacggc cacgggtgct cttgatcctg
    11101 tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt
    11161 cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg
    11221 gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca
    11281 tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc
    11341 agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa
    11401 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa
    11461 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa
    11521 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc
    11581 tctggttctt gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc
    11641 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
    11701 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11761 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11881 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    11941 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    12001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    12061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    12121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    12181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    12241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    12301 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    12361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    12421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    12481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12541 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa ggcattttgg tcatgagatt
    12781 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12841 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12901 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12961 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    13021 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    13081 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    13141 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    13201 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    13261 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    13321 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    13381 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    13441 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    13501 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13561 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13621 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13681 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13741 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13801 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtg
//
