LOCUS       sequence-460518-       13894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH 
            syndrome) under control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, 
            Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T
  TITLE     IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human
            monocytes.
  JOURNAL   iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563.
            eCollection 2025 Jan 17.
  PUBMED    39868044
REFERENCE   2  (bases 1 to 13894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-12-10"; volume: "28(1):111563. doi"; pages: " 
            10.1016/j.isci.2024.111563. eCollection 2025 Jan 17"
COMMENT     Sequence Label: pInducer20 ALPK1 Y254C
FEATURES             Location/Qualifiers
     source          1..13894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1340..1405)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1448..1472)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1581..1605)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1602..1640)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    1673..1691
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1715..1733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1757..1775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1799..1817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1872..1989
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2282..2326)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2511..2744
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3241..3366
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3413..4046
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4238..4257)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4307..4323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4307..4323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4320..4342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4331..4347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4355..4361,4362..4379,4380..4385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4400..4421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4661..4678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4832..5420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5591..6382,6383..6451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6214..6233
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6452..6556)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6624..6642
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             6761..6994
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7069..7657
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7175..7186
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7584..7604
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7834..8628)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7834..8628)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7945..7964)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8555..8574
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    8644..9217
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8804..8823)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9033..9050
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(9270..9278,9279..9314,9315..9317,9318..9353,
                     9354..9356,9357..9392,9393..9398,9399..10016))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(10075..11286)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(10219..10238)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(11372..11398)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    11451..11475
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcttgtcaag acgacattca aagctttcag gcacgtatga gcgatcgtca acgttagaaa
       61 atgactgcac ctgaaggtgc tctttgactt gtgcgataac tgacatcacc tcttgggata
      121 aggcttcccg atcttgagaa cggctactgg ttgaaaagtt atagagttta cccatagcct
      181 ctttgcaaag ttggctagcc gcgtgcacgg tgccggtttc gccgtgcaac cgccgatgaa
      241 cggttgtcag gccaaacgcg gcctttacga aggaatgcaa ttcttgcttg cctgttacgg
      301 gttcgtcgtc ccgtttcgta agaagaccga tttcgaaagc ttcctttgcc tcgcagaggt
      361 gaagattctt cagctcgggc gggcaatcgt tggagctgct atatgacagc aggcatgtac
      421 cccttatgtt gacggcggtc agtacgaaaa gaggggtgta agcgctaaaa gcggcggcca
      481 atttacatgc ctcagcggcg ctgaggagat gatgatcgaa ttctttcaag agggacaggt
      541 tgatctgagg gttattcttg aatttctcgc agtcattctt ggacatgctg acgaaaatat
      601 cagcaaggat gccgaggctg gtactcaacc ctttcttgtc gggttggggc agagcaaggt
      661 agcccacgat actagcccag atcaattcgg cagcctcata ccacattccg agtttctgga
      721 gtatttgtcc cctaatttgg atgcacacgc tttggacaag aactttatct gactcgttcc
      781 tataaagcca agtgccggtc gcaccgttat tagagatcaa gctagagagg atgtattcgg
      841 ccttgagcaa cttaccacta ttaacgctaa tccttgcctg cctgatgacg acttgtggag
      901 ctataggtgt agcgggttga agtttatgaa ggcccttagc cacttggagc agcttaccgg
      961 acacatcaag gccgtacaga aatcgatcta cgagaaagac gatcgcagct gccgcagcgc
     1021 aatctcttgc aagtattgac gcccgaaggc tggccaacag ttgttgcagt ccagcaccga
     1081 tgacgtcttt caaattcgtc ttatcttcgg gcccgactgc ctgcttatat tgccatttct
     1141 cgggaacaaa aggccatttc atctctttgg cttcttgaat gagagttctc agttcggacg
     1201 gaagcagggc cctacacctt tgatcttcgg atttatcttc ctcgctaacg tcaggagcct
     1261 caagaagtaa ttggtcgagg acctgtttac attcttgaag gagcacagcg actactttct
     1321 ggttgttcgc ggccgcaagc ttgtcatcgt catccttgta atcgatgtca tgatctttat
     1381 aatcaccgtc atggtctttg tagtccatgg tggtaccgga tccagtcgac tgaattggtt
     1441 cctttaaagc ctgctttttt gtacaaactt gttgatatct gcagaattcc accacactgg
     1501 actagttcgg ggccgcggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag
     1561 cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc
     1621 tcccaccgta cacgcctacc tcgacccggg taccgagctc gactttcact tttctctatc
     1681 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     1741 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     1801 tctatcactg atagggagtg gtaaaggatc ctagtccaaa ctggatctct gctgtccctg
     1861 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     1921 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     1981 cttttaaaat tgtggatgaa tactgccatt tgtgaattcg gcgataccgt cgagatccgt
     2041 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     2101 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     2161 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     2221 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     2281 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     2341 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     2401 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     2461 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     2521 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     2581 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     2641 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     2701 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     2761 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     2821 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     2881 gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     2941 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     3001 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     3061 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     3121 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     3181 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     3241 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     3301 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     3361 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     3421 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3481 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3541 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     3601 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag
     3661 gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca
     3721 gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc
     3781 aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg
     3841 taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac
     3901 tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt
     3961 ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct
     4021 tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt taacaaatac
     4081 aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa tgaaaccaca
     4141 ggtaattggc agtggtaata gggtatgggg tgggaagttt gggatgattt tggttagctt
     4201 gagttatcca gttgatccag acatgataag atacattgat gagtttggac aaaccacaac
     4261 tagaatgcag tgaaaaaaat gctttatttg tgaaattcgt aatcatgtca tagctgtttc
     4321 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4381 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4441 ccgctttcca gtcgggaaac ctgtcgtgcc acggggagct ttttgcaaaa gcctaggcct
     4501 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     4561 gcataaataa aaaaaattag tcagccatga gcttgctgca ttaatgaatc ggccaacgcg
     4621 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4681 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5461 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5521 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5581 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5641 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5701 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5761 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5821 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5881 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5941 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6001 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6061 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6121 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6181 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     6241 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6301 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6361 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6421 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6481 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6541 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6601 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
     6661 gatctagccc tttccttaat taacccgggc ctctcactct ctgatattca tttctttgca
     6721 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
     6781 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     6841 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     6901 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
     6961 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     7021 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gatctcaggc ggggaggcgg
     7081 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     7141 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     7201 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga aaggacgatg
     7261 atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     7321 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     7381 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     7441 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     7501 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     7561 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag
     7621 tcaatctttc acaaattttg taatccagag gttgattagg atctatcgcg ataagcttga
     7681 tatcgaattg ggagggacta aacaacaaca attgcattca ttttatgttt caggttcagg
     7741 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     7801 atgatctaga gtcgcggccg ctttacttgt acatcagaag aactcgtcaa gaaggcgata
     7861 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     7921 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     7981 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     8041 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     8101 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     8161 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     8221 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     8281 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     8341 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     8401 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     8461 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     8521 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     8581 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgg tggaccggta
     8641 agcttatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg
     8701 tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc
     8761 catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag
     8821 ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt
     8881 tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg
     8941 gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg
     9001 aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc
     9061 cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga
     9121 cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct
     9181 tcggccagta acgttagggg ggggggaggg agaggggggg gggggagagg ggcggaattc
     9241 ctctagtgcg gccgcggatc cttacttagt tacccgggga gcatgtcaag gtcaaaatcg
     9301 tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc
     9361 atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt
     9421 ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc
     9481 cggtcgaaca gctcaattgc ttgtctcaga agtgggggca tagaatcggt ggtaggtgtc
     9541 tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag
     9601 tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg
     9661 aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc
     9721 actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc
     9781 agaaagtctt gccatgactc gccttccagg gggcagaagt gggtatgatg cctgtccagc
     9841 atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta
     9901 ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgaca
     9961 ccattgagta attccagagc tccgtttatg actttgctct tgtccagtct agacatggtg
    10021 aattcgactg caggaccggt acggggtgga gatccgagct cggtaccaag cttcgtctaa
    10081 caaaaaagcc aaaaacggcc agaatttagc ggacaattta ctagtctaac actgaaaatt
    10141 acatattgac ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac aaaacacact
    10201 cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa gataggtaca
    10261 taaaaccgac caaagaaact gacgcctcac ttatccctcc cctcaccaga ggtccggcgc
    10321 ctgtcgattc aggagagcct accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc
    10381 ctaccgcaca cctaccggca ggtggcccca ccctgcatta taagccaaca gaacgggtga
    10441 cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc
    10501 gccataactg ccgcccccgc aacagacgac aaaccgagtt ctccagtcag tgacaaactt
    10561 cacgtcaggg tccccagatg gtgccccagc ccatctcacc cgaataagag ctttcccgca
    10621 ttagcgaagg cctcaagacc ttgggttctt gccgcccacc atgcccccca ccttgtttca
    10681 acgacctcac agcccgcctc acaagcgtct tccattcaag actcgggaac agccgccatt
    10741 ttgctgcgct ccccccaacc cccagttcag ggcaaccttg ctcgcggacc cagactacag
    10801 cccttggcgg tctctccaca cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc
    10861 ccggccagcc cagcagcccg ctactcacca agtgacgatc acagcgatcc acaaacaaga
    10921 accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat
    10981 ataatcatcg gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact
    11041 acttttcctc gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt
    11101 cccgtcctaa aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc
    11161 gggccgctgt cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg
    11221 acgtggcagc gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg
    11281 gaggccagat cttgggtggg ttactccaga ctgccttggg aaaagcgcct cccctacccg
    11341 gtagaatttc tagtttaatt aatcattact aagcgtagtc tgggacgtcg tatgggtatt
    11401 cgaaccgcgg gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt
    11461 acaagaaagc tgggtctaga tatctcgagt caggtacacg gcttttccat gcttggcctt
    11521 gtgagtgaga gtcggtgaca aatctcgttg cactcgacgt gttgattgtt gaagaaatag
    11581 aagatgcccc ttttaccgaa gttagtggta aagacctttt ggtccactga atggatttgt
    11641 gggtcggtca aataaatcag gcccttaccg tttccagtca cccatccctg caggtctacg
    11701 acgacgtccc tgtggttgct gaattcgtat gagaagtgcc cgtatgcgag cccgtactca
    11761 gttgccttat actccgtctt aacgacctta gtattgttgg aaagcttgac gaactcgccg
    11821 aggatatatg gttcaacgct aatgcaacct ttaatggttt tatcttccag tatgagcagg
    11881 atcgtagacg gtatataaaa gatttgagtt ggtatattct gctcgtacag ccttttattg
    11941 aactcagtga cgtaatgttg ggcagtcatt tgcctttcaa cgtccgtaaa atgatgccaa
    12001 aggcctttct gttctttgta atccttacct acgtatcgtc cgagtatctc ctcttggtgc
    12061 aggtggtgca cccagaaggc gtttcgctgt cgacccttct tcttgacggt gagataatcg
    12121 cccaggtaca cgatggtctc ttgagctgtc cacaactcgc tcttcttgga gtacttcaga
    12181 agcagggcgc tgtgagcgcg atgcagctgg gacggtttga atacacctgt gttttcgagc
    12241 ctttggaaaa gccagtcgtg gcggacgccg gccagaagtt tctcgaaatc gtcgggctgc
    12301 agtgttcgtg cttcgagaat ttcctttcgc attttaccag gcagagagac ccagctgctg
    12361 cctgaagagt tgaggtaact ccatggagaa tcgccttctg aggacccgct actgaacgct
    12421 ggggacttca accaccagac agagctagag gaactatttt gactacaatt caacatattg
    12481 cctggttggt tgccctcttc ggttgttgta caatcttctg aaagcacggg gaaaagtata
    12541 ttccctgaca ctgaactgtt tggtgtttca gctctttgtc ctgggggttg cctgagtata
    12601 cataatcggt gactaccatg gccgttggta caaggcacgt ccatagaatc cagcaactgt
    12661 ccttcttcgt caacggtaga agcgtcggga tcaatccctt gttcggctac gggcccgccg
    12721 ctcttgcgtg aatcggggtt ctggacgggc cagtttggag taaagcttga gcaagggagc
    12781 atgctgatat tcccgaggga gttatgcaaa acacgatgga aatccaatgg ggcgtcctcg
    12841 gtggactcag cagtctcgcc ctcaggatcc acccaactcg gtgaggcctt gaacgttggc
    12901 ccagctcccc tctcagagat ctcctcgcct tgttccttgc cttgccgatc cttcacgtca
    12961 caattagtct cggggaattc cacgatgatc tcgaatgcct cttccttctg cacgctgggg
    13021 tgcgtaccca tgttcttagg gcggccggag tcggagctcc atgatgctga cctgtaactt
    13081 ggcctagaat gtgcgctggt gttccgaggg cccatgttcc ttacctcttg aatgccctcg
    13141 ggcgcgccct caagcaggcc agctccgggc gccagaaaga taccaggcgt gttgtgggga
    13201 ctaaaaggag taagcggcat ttgctgttga ggttggttct ggctcggttc caggttatcg
    13261 ttatttggct cttgcaagct caggtcgtgc agttgggagt gaagactgtg catggcccgc
    13321 ccttcccgat cgttttccag ttcttcgctc agagcggtag agcattgtgt atcaacgagg
    13381 tgctccttcc ctggttcctt cctcgcggat ctatcatcca catggtagtt tacctcttcc
    13441 caggacgcgc tgcttgagaa gccactcaga ttagaccacg cggatgatgt ttgggaccca
    13501 gaaagggatt tattaaacac ggccccttct ccgttagaat aatctgaggg ttctgtttct
    13561 gtttcgacgt cctggtccag gctcacgcgg aaggcgtcgc tgtgtgtcca attccgtctg
    13621 ccacctctcc gcagctctct ttgcacgttc tttcccatca gagagctgct gatcccggta
    13681 tcgcgctggc agtggggttt ctcctgggtt gtgctgacgg tgtcgatatt cttaatctca
    13741 gtcttcagtg cggttataca aacaccggtc ttggcgtcct tttgctcgtt cttattattg
    13801 ccgcagtcgc tctcgaacac ctcgcagaca ctggtgtgat gttgagagta agtatcgaga
    13861 atcttctgaa agtctccctg gccgtgaaga atca
//
