LOCUS       sequence-460523-       13894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ALPK1 WT gene under control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, 
            Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T
  TITLE     IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human
            monocytes.
  JOURNAL   iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563.
            eCollection 2025 Jan 17.
  PUBMED    39868044
REFERENCE   2  (bases 1 to 13894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-12-10"; volume: "28(1):111563. doi"; pages: " 
            10.1016/j.isci.2024.111563. eCollection 2025 Jan 17"
COMMENT     Sequence Label: pInducer20 ALPK1 WT
FEATURES             Location/Qualifiers
     source          1..13894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1700..1765)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1808..1832)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1941..1965)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1962..2000)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2033..2051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2075..2093
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2117..2135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2232..2349
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2642..2686)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2871..3104
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3601..3726
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3773..4406
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4598..4617)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4667..4683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4667..4683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4680..4702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4691..4707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4715..4721,4722..4739,4740..4745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4760..4781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5021..5038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5192..5780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5951..6742,6743..6811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6574..6593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6812..6916)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6984..7002
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             7121..7354
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7429..8017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7535..7546
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7944..7964
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8194..8988)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8194..8988)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8305..8324)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8915..8934
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    9004..9577
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9164..9183)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9393..9410
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(9630..9638,9639..9674,9675..9677,9678..9713,
                     9714..9716,9717..9752,9753..9758,9759..10376))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(10435..11646)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(10579..10598)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(11732..11758)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    11811..11835
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagaataatc tgagggttct gtttctgttt cgacgtcctg gtccaggctc acgcggaagg
       61 cgtcgctgtg tgtccaattc cgtctgccac ctctccgcag ctctctttgc acgttctttc
      121 ccatcagaga gctgctgatc ccggtatcgc gctggcagtg gggtttctcc tgggttgtgc
      181 tgacggtgtc gatattctta atctcagtct tcagtgcggt tatacaaaca ccggtcttgg
      241 cgtccttttg ctcgttctta ttattgccgc agtcgctctc gaacacctcg cagacactgg
      301 tgtgatgttg agagtaagta tcgagaatct tctgaaagtc tccctggccg tgaagaatca
      361 gcttgtcaag acgacattca aagctttcag gcacgtatga gcgatcgtca acgttagaaa
      421 atgactgcac ctgaaggtgc tctttgactt gtgcgataac tgacatcacc tcttgggata
      481 aggcttcccg atcttgagaa cggctactgg ttgaaaagtt atagagttta cccatagcct
      541 ctttgcaaag ttggctagcc gcgtgcacgg tgccggtttc gccgtgcaac cgccgatgaa
      601 cggttgtcag gccaaacgcg gcctttacga aggaatgcaa ttcttgcttg cctgttacgg
      661 gttcgtcgtc ccgtttcgta agaagaccga tttcgaaagc ttcctttgcc tcgcagaggt
      721 gaagattctt cagctcgggc gggcaatcgt tggagctgct atatgacagc aggcatgtac
      781 cccttatgtt gacggcggtc agtacgaaaa gaggggtgta agcgctaaaa gcggcggcca
      841 atttacatgc ctcagcggcg ctgaggagat gatgatcgaa ttctttcaag agggacaggt
      901 tgatctgagg gttattcttg aatttctcgt agtcattctt ggacatgctg acgaaaatat
      961 cagcaaggat gccgaggctg gtactcaacc ctttcttgtc gggttggggc agagcaaggt
     1021 agcccacgat actagcccag atcaattcgg cagcctcata ccacattccg agtttctgga
     1081 gtatttgtcc cctaatttgg atgcacacgc tttggacaag aactttatct gactcgttcc
     1141 tataaagcca agtgccggtc gcaccgttat tagagatcaa gctagagagg atgtattcgg
     1201 ccttgagcaa cttaccacta ttaacgctaa tccttgcctg cctgatgacg acttgtggag
     1261 ctataggtgt agcgggttga agtttatgaa ggcccttagc cacttggagc agcttaccgg
     1321 acacatcaag gccgtacaga aatcgatcta cgagaaagac gatcgcagct gccgcagcgc
     1381 aatctcttgc aagtattgac gcccgaaggc tggccaacag ttgttgcagt ccagcaccga
     1441 tgacgtcttt caaattcgtc ttatcttcgg gcccgactgc ctgcttatat tgccatttct
     1501 cgggaacaaa aggccatttc atctctttgg cttcttgaat gagagttctc agttcggacg
     1561 gaagcagggc cctacacctt tgatcttcgg atttatcttc ctcgctaacg tcaggagcct
     1621 caagaagtaa ttggtcgagg acctgtttac attcttgaag gagcacagcg actactttct
     1681 ggttgttcgc ggccgcaagc ttgtcatcgt catccttgta atcgatgtca tgatctttat
     1741 aatcaccgtc atggtctttg tagtccatgg tggtaccgga tccagtcgac tgaattggtt
     1801 cctttaaagc ctgctttttt gtacaaactt gttgatatct gcagaattcc accacactgg
     1861 actagttcgg ggccgcggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag
     1921 cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc
     1981 tcccaccgta cacgcctacc tcgacccggg taccgagctc gactttcact tttctctatc
     2041 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     2101 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     2161 tctatcactg atagggagtg gtaaaggatc ctagtccaaa ctggatctct gctgtccctg
     2221 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     2281 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     2341 cttttaaaat tgtggatgaa tactgccatt tgtgaattcg gcgataccgt cgagatccgt
     2401 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     2461 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     2521 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     2581 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     2641 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     2701 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     2761 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     2821 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     2881 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     2941 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     3001 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     3061 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     3121 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     3181 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     3241 gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     3301 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     3361 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     3421 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     3481 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     3541 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     3601 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     3661 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     3721 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     3781 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     3841 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     3901 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     3961 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag
     4021 gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca
     4081 gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc
     4141 aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg
     4201 taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac
     4261 tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt
     4321 ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct
     4381 tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt taacaaatac
     4441 aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa tgaaaccaca
     4501 ggtaattggc agtggtaata gggtatgggg tgggaagttt gggatgattt tggttagctt
     4561 gagttatcca gttgatccag acatgataag atacattgat gagtttggac aaaccacaac
     4621 tagaatgcag tgaaaaaaat gctttatttg tgaaattcgt aatcatgtca tagctgtttc
     4681 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4741 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4801 ccgctttcca gtcgggaaac ctgtcgtgcc acggggagct ttttgcaaaa gcctaggcct
     4861 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     4921 gcataaataa aaaaaattag tcagccatga gcttgctgca ttaatgaatc ggccaacgcg
     4981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6541 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6961 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
     7021 gatctagccc tttccttaat taacccgggc ctctcactct ctgatattca tttctttgca
     7081 agttataaat actgaataat aagatgacat gaactactac tgctagagat tttccacact
     7141 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     7201 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     7261 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga
     7321 tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg
     7381 ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gatctcaggc ggggaggcgg
     7441 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     7501 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     7561 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga aaggacgatg
     7621 atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     7681 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     7741 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     7801 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     7861 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     7921 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag
     7981 tcaatctttc acaaattttg taatccagag gttgattagg atctatcgcg ataagcttga
     8041 tatcgaattg ggagggacta aacaacaaca attgcattca ttttatgttt caggttcagg
     8101 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     8161 atgatctaga gtcgcggccg ctttacttgt acatcagaag aactcgtcaa gaaggcgata
     8221 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     8281 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     8341 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     8401 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     8461 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     8521 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     8581 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     8641 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     8701 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     8761 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     8821 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     8881 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     8941 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgg tggaccggta
     9001 agcttatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg
     9061 tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc
     9121 catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag
     9181 ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt
     9241 tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg
     9301 gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg
     9361 aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc
     9421 cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga
     9481 cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct
     9541 tcggccagta acgttagggg ggggggaggg agaggggggg gggggagagg ggcggaattc
     9601 ctctagtgcg gccgcggatc cttacttagt tacccgggga gcatgtcaag gtcaaaatcg
     9661 tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc
     9721 atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt
     9781 ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc
     9841 cggtcgaaca gctcaattgc ttgtctcaga agtgggggca tagaatcggt ggtaggtgtc
     9901 tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag
     9961 tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg
    10021 aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc
    10081 actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc
    10141 agaaagtctt gccatgactc gccttccagg gggcagaagt gggtatgatg cctgtccagc
    10201 atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta
    10261 ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgaca
    10321 ccattgagta attccagagc tccgtttatg actttgctct tgtccagtct agacatggtg
    10381 aattcgactg caggaccggt acggggtgga gatccgagct cggtaccaag cttcgtctaa
    10441 caaaaaagcc aaaaacggcc agaatttagc ggacaattta ctagtctaac actgaaaatt
    10501 acatattgac ccaaatgatt acatttcaaa aggtgcctaa aaaacttcac aaaacacact
    10561 cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc tacttaagaa gataggtaca
    10621 taaaaccgac caaagaaact gacgcctcac ttatccctcc cctcaccaga ggtccggcgc
    10681 ctgtcgattc aggagagcct accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc
    10741 ctaccgcaca cctaccggca ggtggcccca ccctgcatta taagccaaca gaacgggtga
    10801 cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc
    10861 gccataactg ccgcccccgc aacagacgac aaaccgagtt ctccagtcag tgacaaactt
    10921 cacgtcaggg tccccagatg gtgccccagc ccatctcacc cgaataagag ctttcccgca
    10981 ttagcgaagg cctcaagacc ttgggttctt gccgcccacc atgcccccca ccttgtttca
    11041 acgacctcac agcccgcctc acaagcgtct tccattcaag actcgggaac agccgccatt
    11101 ttgctgcgct ccccccaacc cccagttcag ggcaaccttg ctcgcggacc cagactacag
    11161 cccttggcgg tctctccaca cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc
    11221 ccggccagcc cagcagcccg ctactcacca agtgacgatc acagcgatcc acaaacaaga
    11281 accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc cacacccggc gcgtccttat
    11341 ataatcatcg gcgttcaccg ccccacggag atccctccgc agaatcgccg agaagggact
    11401 acttttcctc gcctgttccg ctctctggaa agaaaaccag tgccctagag tcacccaagt
    11461 cccgtcctaa aatgtccttc tgctgatact ggggttctaa ggccgagtct tatgagcagc
    11521 gggccgctgt cctgagcgtc cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg
    11581 acgtggcagc gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg
    11641 gaggccagat cttgggtggg ttactccaga ctgccttggg aaaagcgcct cccctacccg
    11701 gtagaatttc tagtttaatt aatcattact aagcgtagtc tgggacgtcg tatgggtatt
    11761 cgaaccgcgg gccctctaga ctcgagcggc cgccactgtg ctggatatca accactttgt
    11821 acaagaaagc tgggtctaga tatctcgagt caggtacacg gcttttccat gcttggcctt
    11881 gtgagtgaga gtcggtgaca aatctcgttg cactcgacgt gttgattgtt gaagaaatag
    11941 aagatgcccc ttttaccgaa gttagtggta aagacctttt ggtccactga atggatttgt
    12001 gggtcggtca aataaatcag gcccttaccg tttccagtca cccatccctg caggtctacg
    12061 acgacgtccc tgtggttgct gaattcgtat gagaagtgcc cgtatgcgag cccgtactca
    12121 gttgccttat actccgtctt aacgacctta gtattgttgg aaagcttgac gaactcgccg
    12181 aggatatatg gttcaacgct aatgcaacct ttaatggttt tatcttccag tatgagcagg
    12241 atcgtagacg gtatataaaa gatttgagtt ggtatattct gctcgtacag ccttttattg
    12301 aactcagtga cgtaatgttg ggcagtcatt tgcctttcaa cgtccgtaaa atgatgccaa
    12361 aggcctttct gttctttgta atccttacct acgtatcgtc cgagtatctc ctcttggtgc
    12421 aggtggtgca cccagaaggc gtttcgctgt cgacccttct tcttgacggt gagataatcg
    12481 cccaggtaca cgatggtctc ttgagctgtc cacaactcgc tcttcttgga gtacttcaga
    12541 agcagggcgc tgtgagcgcg atgcagctgg gacggtttga atacacctgt gttttcgagc
    12601 ctttggaaaa gccagtcgtg gcggacgccg gccagaagtt tctcgaaatc gtcgggctgc
    12661 agtgttcgtg cttcgagaat ttcctttcgc attttaccag gcagagagac ccagctgctg
    12721 cctgaagagt tgaggtaact ccatggagaa tcgccttctg aggacccgct actgaacgct
    12781 ggggacttca accaccagac agagctagag gaactatttt gactacaatt caacatattg
    12841 cctggttggt tgccctcttc ggttgttgta caatcttctg aaagcacggg gaaaagtata
    12901 ttccctgaca ctgaactgtt tggtgtttca gctctttgtc ctgggggttg cctgagtata
    12961 cataatcggt gactaccatg gccgttggta caaggcacgt ccatagaatc cagcaactgt
    13021 ccttcttcgt caacggtaga agcgtcggga tcaatccctt gttcggctac gggcccgccg
    13081 ctcttgcgtg aatcggggtt ctggacgggc cagtttggag taaagcttga gcaagggagc
    13141 atgctgatat tcccgaggga gttatgcaaa acacgatgga aatccaatgg ggcgtcctcg
    13201 gtggactcag cagtctcgcc ctcaggatcc acccaactcg gtgaggcctt gaacgttggc
    13261 ccagctcccc tctcagagat ctcctcgcct tgttccttgc cttgccgatc cttcacgtca
    13321 caattagtct cggggaattc cacgatgatc tcgaatgcct cttccttctg cacgctgggg
    13381 tgcgtaccca tgttcttagg gcggccggag tcggagctcc atgatgctga cctgtaactt
    13441 ggcctagaat gtgcgctggt gttccgaggg cccatgttcc ttacctcttg aatgccctcg
    13501 ggcgcgccct caagcaggcc agctccgggc gccagaaaga taccaggcgt gttgtgggga
    13561 ctaaaaggag taagcggcat ttgctgttga ggttggttct ggctcggttc caggttatcg
    13621 ttatttggct cttgcaagct caggtcgtgc agttgggagt gaagactgtg catggcccgc
    13681 ccttcccgat cgttttccag ttcttcgctc agagcggtag agcattgtgt atcaacgagg
    13741 tgctccttcc ctggttcctt cctcgcggat ctatcatcca catggtagtt tacctcttcc
    13801 caggacgcgc tgcttgagaa gccactcaga ttagaccacg cggatgatgt ttgggaccca
    13861 gaaagggatt tattaaacac ggccccttct ccgt
//
