LOCUS       sequence-460526-       10842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TIFA WT gene under control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, 
            Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T
  TITLE     IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human
            monocytes.
  JOURNAL   iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563.
            eCollection 2025 Jan 17.
  PUBMED    39868044
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-12-10"; volume: "28(1):111563. doi"; pages: " 
            10.1016/j.isci.2024.111563. eCollection 2025 Jan 17"
COMMENT     Sequence Label: pInducer20 TIFA WT
FEATURES             Location/Qualifiers
     source          1..10842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        145..183
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     180..204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    313..337
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             380..445
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1024..1048)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1101..1127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1213..2424
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2261..2280
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2483..3100,3101..3106,3107..3142,3143..3145,3146..3181,
                     3182..3184,3185..3220,3221..3229)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3282..3855
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3449..3466)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3676..3695
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3871..4665
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3871..4665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3925..3944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4535..4554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4842..5430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4895..4915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5313..5324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5505..5738
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5857..5875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5943..6047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6048..6116,6117..6908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6266..6285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7079..7667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7568..7587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7821..7838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8078..8099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8114..8119,8120..8137,8138..8144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8152..8168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8157..8179
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8176..8192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8176..8192
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8242..8261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8453..9086
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9133..9258
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9755..9988
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10173..10217
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10510..10627
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(10682..10700,10701..10723,10724..10742,10743..10765,
                     10766..10784,10785..10807,10808..10826,10827..10842,1..9,
                     10..28,29..51,52..70,71..93,94..112)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
       61 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      121 agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
      181 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      241 agaagacacc gggaccgatc cagcctccgc ggccccgaac tagtccagtg tggtggaatt
      301 ctgcagatat caacaagttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      361 tggatccggt accaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      421 cgattacaag gatgacgatg acaagcttgc ggccgcgact tccttcgagg acgccgatac
      481 tgaggaaacc gtcacctgcc tgcaaatgac cgtatatcac ccaggtcaac tccaatgcgg
      541 catcttccaa agcatttcct tcaatcgcga aaagcttcca agttctgagg tagtcaagtt
      601 cggtcgcaac tctaatatat gccactacac cttccaagat aagcaagtgt ctcgggtaca
      661 attctccctt caactcttca agaagtttaa ttctagtgtg ctgagtttcg agattaagaa
      721 catgtcaaag aaaactaact tgattgtcga ttctcgggaa ttggggtatt tgaacaagat
      781 ggatctccct tatcgctgta tggtgaggtt tggggaatac caattcctta tggaaaagga
      841 agacggagaa tccctggagt tcttcgaaac acagttcatc ctgagtccca ggagcctgct
      901 ccaggagaat aattggccgc cgcatcgacc aatccccgaa tacggaacct actcactgtg
      961 ttctagtcag tcttcttcac ccaccgagat ggacgagaac gaaagctagc tcgagatatc
     1021 tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
     1081 tagagggccc gcggttcgaa tacccatacg acgtcccaga ctacgcttag taatgattaa
     1141 ttaaactaga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagtaacc
     1201 cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     1261 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     1321 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     1381 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     1441 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     1501 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     1561 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     1621 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     1681 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     1741 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     1801 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     1861 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     1921 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     1981 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     2041 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     2101 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     2161 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     2221 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     2281 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     2341 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     2401 ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc caccccgtac
     2461 cggtcctgca gtcgaattca ccatgtctag actggacaag agcaaagtca taaacggagc
     2521 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     2581 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     2641 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     2701 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     2761 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     2821 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     2881 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     2941 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     3001 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     3061 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     3121 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     3181 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     3241 tccgcggccg cactagagga attccgcccc tctccccccc ccccctctcc ctcccccccc
     3301 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3361 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3421 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     3481 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3541 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     3601 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     3661 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     3721 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     3781 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     3841 aaaacacgat gataagctta ccggtccacc atgattgaac aagatggatt gcacgcaggt
     3901 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3961 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4021 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4081 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4141 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4201 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4261 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4321 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4381 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4441 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4501 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4561 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4621 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgatgtac aagtaaagcg
     4681 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4741 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttta
     4801 gtccctccca attcgatatc aagcttatcg cgatagatcc taatcaacct ctggattaca
     4861 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4921 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4981 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5041 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5101 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5161 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5221 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5281 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5341 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5401 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     5461 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5521 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5581 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5641 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5701 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5761 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     5821 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     5881 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5941 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6001 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6061 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6121 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6181 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6241 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     6301 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6361 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6421 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6481 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6541 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6601 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6661 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6721 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6781 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6841 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6901 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6961 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7021 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7081 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7141 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7201 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7261 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7321 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7381 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7441 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7501 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7561 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7621 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7681 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7741 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7801 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7861 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     7921 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7981 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     8041 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     8101 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     8161 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
     8221 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8281 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     8341 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     8401 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     8461 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     8521 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     8581 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     8641 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8701 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     8761 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     8821 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     8881 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     8941 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9001 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9061 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     9121 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     9181 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     9241 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     9301 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     9361 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     9421 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     9481 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     9541 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     9601 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     9661 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9721 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9781 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9841 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9901 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9961 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    10021 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    10081 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    10141 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    10201 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    10261 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    10321 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    10381 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    10441 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt
    10501 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    10561 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10621 aaattttcgg gtttattaca gggacagcag agatccagtt tggactagga tcctttacca
    10681 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
    10741 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
    10801 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gt
//
