LOCUS       sequence-460528-       10840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TIFA gene mutated in position T9A (mutation on phosphorylation site)
            under control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, 
            Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T
  TITLE     IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human
            monocytes.
  JOURNAL   iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563.
            eCollection 2025 Jan 17.
  PUBMED    39868044
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-12-10"; volume: "28(1):111563. doi"; pages: " 
            10.1016/j.isci.2024.111563. eCollection 2025 Jan 17"
COMMENT     Sequence Label: pInducer20 TIFA T9A
FEATURES             Location/Qualifiers
     source          1..10840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..181
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     178..202
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    311..335
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             378..443
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1022..1046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1099..1125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1211..2422
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2259..2278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2481..3098,3099..3104,3105..3140,3141..3143,3144..3179,
                     3180..3182,3183..3218,3219..3227)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3280..3853
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3447..3464)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3674..3693
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3869..4663
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3869..4663
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3923..3942)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4533..4552
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4840..5428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4893..4913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5311..5322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5503..5736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5855..5873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5941..6045
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6046..6114,6115..6906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6264..6283)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7077..7665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7566..7585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7819..7836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8076..8097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8112..8117,8118..8135,8136..8142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8150..8166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8155..8177
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8174..8190
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8174..8190
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8240..8259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8451..9084
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9131..9256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9753..9986
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10171..10215
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10508..10625
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(10680..10698,10699..10721,10722..10740,10741..10763,
                     10764..10782,10783..10805,10806..10824,10825..10840,1..7,
                     8..26,27..49,50..68,69..91,92..110)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
       61 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      121 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      181 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      241 aagacaccgg gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct
      301 gcagatatca acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg
      361 gatccggtac caccatggac tacaaagacc atgacggtga ttataaagat catgacatcg
      421 attacaagga tgacgatgac aagcttgcgg ccgcgacttc cttcgaggac gccgatgctg
      481 aggaaaccgt cacctgcctg caaatgaccg tatatcaccc aggtcaactc caatgcggca
      541 tcttccaaag catttccttc aatcgcgaaa agcttccaag ttctgaggta gtcaagttcg
      601 gtcgcaactc taatatatgc cactacacct tccaagataa gcaagtgtct cgggtacaat
      661 tctcccttca actcttcaag aagtttaatt ctagtgtgct gagtttcgag attaagaaca
      721 tgtcaaagaa aactaacttg attgtcgatt ctcgggaatt ggggtatttg aacaagatgg
      781 atctccctta tcgctgtatg gtgaggtttg gggaatacca attccttatg gaaaaggaag
      841 acggagaatc cctggagttc ttcgaaacac agttcatcct gagtcccagg agcctgctcc
      901 aggagaataa ttggccgccg catcgaccaa tccccgaata cggaacctac tcactgtgtt
      961 ctagtcagtc ttcttcaccc accgagatgg acgagaacga aagctagctc gagatatcta
     1021 gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     1081 gagggcccgc ggttcgaata cccatacgac gtcccagact acgcttagta atgattaatt
     1141 aaactagaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagtaaccca
     1201 cccaagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     1261 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     1321 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     1381 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     1441 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     1501 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     1561 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     1621 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     1681 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     1741 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     1801 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     1861 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     1921 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     1981 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     2041 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     2101 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     2161 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     2221 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     2281 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     2341 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     2401 gtttttggct tttttgttag acgaagcttg gtaccgagct cggatctcca ccccgtaccg
     2461 gtcctgcagt cgaattcacc atgtctagac tggacaagag caaagtcata aacggagctc
     2521 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     2581 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     2641 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     2701 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     2761 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     2821 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     2881 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     2941 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     3001 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     3061 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
     3121 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     3181 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc
     3241 cgcggccgca ctagaggaat tccgcccctc tccccccccc ccctctccct cccccccccc
     3301 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3361 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3421 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3481 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3541 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3601 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3661 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3721 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3781 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     3841 aacacgatga taagcttacc ggtccaccat gattgaacaa gatggattgc acgcaggttc
     3901 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3961 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4021 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     4081 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4141 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4201 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4261 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4321 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     4381 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4441 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4501 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4561 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4621 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgatgtacaa gtaaagcggc
     4681 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4741 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgtttagt
     4801 ccctcccaat tcgatatcaa gcttatcgcg atagatccta atcaacctct ggattacaaa
     4861 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4921 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4981 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5041 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5101 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5161 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5221 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     5281 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5341 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5401 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     5461 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5521 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5581 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5641 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5701 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5761 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     5821 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5881 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5941 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6001 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6061 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6121 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6181 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6241 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6301 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6361 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6421 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6481 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6541 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6601 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6661 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6721 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6781 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6841 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6901 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6961 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7021 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7741 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7801 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7861 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
     7921 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7981 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
     8041 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8101 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8161 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
     8221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8281 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     8341 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     8401 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     8461 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     8521 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     8581 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     8641 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     8701 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     8761 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     8821 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     8881 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9061 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     9121 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     9181 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     9241 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     9301 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     9361 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     9421 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     9481 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     9541 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     9601 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9661 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9721 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9781 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9841 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9901 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9961 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    10021 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    10081 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    10141 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    10201 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    10261 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    10321 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    10381 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    10441 tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca
    10501 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    10561 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10621 attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc ctttaccact
    10681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
    10741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
    10801 accactccct atcagtgata gagaaaagtg aaagtcgagt
//
