LOCUS       sequence-46053-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Photo-activated Caspase-7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L57R
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..132
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             159..587
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 caattccccg gagttaatcc gggaccttta attcaaccca acacaatata ttatagttaa
       61 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
      121 ttatttacaa tcaaaggaga tataccatgg ggactagttt ggctactaca cttgaacgta
      181 ttgagaagaa ctttgtcatt actgacccaa gattgccaga taatcccatt atattcgcgt
      241 ccgatagttt cttgcagttg acagaatata gccgtgaaga aattttggga agaaactgca
      301 ggtttctaca aggtcctgaa actgatcgcg cgacagtgag aaaaattaga gatgccatag
      361 ataaccaaac agaggtcact gttcagctga ttaattatac aaagagtggt aaaaagttct
      421 ggaacctctt tcacttgcag cctatgcgag atcagaaggg agatgtccag tactttattg
      481 gggttcagtt ggatggaact gagcatgtcc gagatgctgc cgagagagag ggagtcatgc
      541 tgattaagaa aactgcagaa aatattgatg aggcggcaaa agaacttgct agtggatcca
      601 catatcagta caacatgaat tttgaaaagc tgggcaaatg catcataata aacaacaaga
      661 actttgataa agtgacaggt atgggcgttc gaaacggaac agacaaagat gccgaggcgc
      721 tcttcaagtg cttccgaagc ctgggttttg acgtgattgt ctataatgac tgctcttgtg
      781 ccaagataca agatctgctt aaaaaagctt ctgaagagga ccatacaaat gccgcctgct
//
