LOCUS       sequence-46054-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Mills E, Truong K
  TITLE     Ca2+-mediated synthetic biosystems offer protein design versatility,
            signal specificity, and pathway rewiring.
  JOURNAL   Chem Biol. 2011 Dec 23;18(12):1611-9.
  PUBMED    22195563
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2011-12-23"; volume: "18(12)"; pages: "1611-9"
COMMENT     Sequence Label: CaRQ
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        57..166
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             628..705
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atcgactcac tataggggaa ttgtgagcgg ataacaattc cccggagtta atccgggacc
       61 tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca aatcatttgt
      121 atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag gagatatacc
      181 atgggcctga ctagtgagca gattgcagag ttcaaagaag ccttctcatt attcgacaag
      241 gatggggacg gcaccatcac cacaaaggaa cttggcaccg ttatgaggtc gcttggacaa
      301 aacccaacgg aagcagaatt gcaggatatg atcaatgaag tcgatgctga tggcaatgga
      361 acgatttact ttcctgaatt tcttactatg atggctagaa aaatgaagga cacagacagc
      421 gaagaggaaa tccgagaagc attccgtgtt tttgacaagg atgggaacgg ctacatcagc
      481 gctgctgaat tacgtcacgt catgacaaac ctcggggaga agttaacaga tgaagaagtt
      541 gatgaaatga taagggaagc agatatcgat ggtgatggcc aagtaaacta tgaagagttt
      601 gtacaaatga tgacagcaaa ggctagtaag aggcgctgga agaaaaactt cattgccgtc
      661 agcgctgcca accggttcaa gaagatctcc agctccgggg cactggctag tgctgccatc
      721 cggaagaaac tggtgattgt tggtgatgga gcctgtggaa agacatgctt gctcatagtc
      781 ttcagcaagg accagttccc agaggtgtat gtgcccacag tgtttgagaa ctatgtggca
      841 gatatcga
//
