LOCUS       sequence-460571-        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 
            apoptosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Hanggi K, Li J, Gangadharan A, Liu X, Celias DP, Osunmakinde O, 
            Keske A, Davis J, Ahmad F, Giron A, Anadon CM, Gardner A, DeNardo 
            DG, Shaw TI, Beg AA, Yu X, Ruffell B
  TITLE     Interleukin-1alpha release during necrotic-like cell death generates
            myeloid-driven immunosuppression that restricts anti-tumor immunity.
  JOURNAL   Cancer Cell. 2024 Nov 13:S1535-6108(24)00402-1. doi: 
            10.1016/j.ccell.2024.10.014.
  PUBMED    39577420
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2024 Nov 13:S1535-6108(24)00402-1. doi: 
            10.1016/j.ccell.2024.10.014."
COMMENT     Sequence Label: pLVX-DmrB-Casp8-2A-mCherry-puro
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    144..377
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    874..999
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1046..1679
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1847..1981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1871..1890)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1925..1944
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2029..2133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2134..2202,2203..2994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2352..2371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3165..3753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3654..3673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3907..3924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4041..4062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4077..4082,4083..4100,4101..4107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4115..4131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4120..4142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4139..4155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4139..4155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4283..4916
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5123..5711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5229..5240
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5638..5658
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5728..6327)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5811..5831)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6308..6327
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6348..6847)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6396..6415)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6789..6812
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(6869..7576)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7144..7163)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7413..7431
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7577..7630)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(8504..8824)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(8855..8896)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     promoter        complement(8909..9258)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(8946..8970)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9016..9034
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9052..9070
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9088..9106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9124..9142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9160..9178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9196..9214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9232..9250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    9321..9436
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9699..9743)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
       61 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
      121 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
      181 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
      241 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
      301 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
      361 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
      421 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
      481 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg
      541 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
      601 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
      661 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
      721 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
      781 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
      841 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
      901 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
      961 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     1021 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     1081 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     1141 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     1201 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     1261 tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc
     1321 ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc
     1381 tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc
     1441 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt
     1501 ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag
     1561 tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt
     1621 gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttccaa
     1681 ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag
     1741 aggaatcaca ggtttagagt aaatgaaacc acaggtaatt ggcagtggta atagggtatg
     1801 gggtgggaag tttgggatga ttttggttag cttgagttat ccagttgatc cagacatgat
     1861 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     1921 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     1981 tgatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2041 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2101 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2161 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2221 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2281 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2341 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2401 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2461 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2521 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2581 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     2641 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2701 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2761 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2821 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2881 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2941 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3001 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3061 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3121 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3181 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3241 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3301 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3361 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3421 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3481 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3541 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3601 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3661 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3721 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3781 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3841 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3901 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3961 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4021 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4081 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     4141 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg taaacgctag
     4201 caatgtcaag gcctctcact ctctgatatt catttctttg caagttataa atactgaata
     4261 ataagatgac atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg
     4321 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     4381 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     4441 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct
     4501 gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt ccccagcgga
     4561 aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat gcagctctcg
     4621 ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt ctctctccgg
     4681 gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc ctttattggc
     4741 ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa aggtcagtgg
     4801 atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg
     4861 gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc cttccagtcc
     4921 cctcttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     4981 aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc
     5041 acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt tttctaggtc
     5101 tcgactgcag aattaattcc aggcggggag gcggcccaaa gggagatccg actcgtctga
     5161 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     5221 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     5281 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
     5341 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     5401 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     5461 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     5521 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     5581 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     5641 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     5701 agaggttgat tgttccagac gcgcccgtca ggcaccgggc ttgcgggtca tgcaccaggt
     5761 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     5821 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     5881 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     5941 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     6001 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     6061 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     6121 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     6181 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     6241 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     6301 gcgcaccgtg ggcttgtact cggtcatggt aagcttgggc tgcaggtcga aaggcccgga
     6361 gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg
     6421 cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag cccgcccccg
     6481 gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg ctattggccg
     6541 ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg cacgagacta
     6601 gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg
     6661 aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc
     6721 ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc
     6781 caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa aagcgcctcc
     6841 cctacccggt atcagtcagt cagaattcct tgtacagctc gtccatgccg ccggtggagt
     6901 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
     6961 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
     7021 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
     7081 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
     7141 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
     7201 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
     7261 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
     7321 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
     7381 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
     7441 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     7501 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     7561 cgcccttgct caccataggg ccgggattct cctccacgtc accgcatgtt agaagacttc
     7621 ctctgccctc gaattccata tgatcagaag ggaagacaag tttttttctt agtgtgaaag
     7681 taggctgagg catctgtttc cccatgtttt tcttgtcatc cttgttgctt acttcatagt
     7741 tcacttcagt caggatggtg agaatatcat cgcctcgagg acatcgctct ctcaggctct
     7801 ggcaaagtga ctggatgtac caggttccct ctgcagggtt tcggtaggaa acacagttat
     7861 tcacagtggc catccccagc agaaagtcag cctcatccgg gatatatctc gtttgaggtg
     7921 atgataaatc catttctaaa tagggttgct cctctgaatc agtctcaaca ggtatacctt
     7981 tctggtagtt atccccctga caagcctgaa taaaaaacac tttgggtttt ccagcaaggg
     8041 aagggcactt caaaccagtg aactgagatg tcagctcata gatgggggcc tcctgtccat
     8101 cagtgccata gatgatgccc ttgtctccat gggagaggat acagcagatg aagcagtcca
     8161 tgttactgtg gtccatgagt tggtagattt tcaaaatctc atagatttgc tctactgtgc
     8221 agtcatcgtg gggcttgatc tcaaaatgaa gctcttcaaa ggtcgtggtc aaagcccctg
     8281 catccaagtg tgttccattc ctgtccctaa tgctgtgaag tttgggcact ttctcccgtg
     8341 cttttgcaaa attgtgattg ttgatgatca gacagtatcc ccgaggtttg cttttcattt
     8401 ggtaaacttt gtccaaagtc tgtgattcac tatcctgttc tcttggagag tccgagattg
     8461 tcatatggga tcctctagaa cctccacctc ccatatgacg cgtttccagt tttagaagct
     8521 ccacatcgaa gacgagagtg gcatgtggtg ggatgatgcc tgggtgccca gtggcaccat
     8581 aggcataatc tggagatata gtcagtttgg ctctctgacc cacactcatc tgggcaaccc
     8641 cttcttccca gcctcggatc acctcctgct tgcctagcat aaacttaaag ggcttgtttc
     8701 tgtcccggga ggaatcaact ttctttccat cttcaagcat cccggtgtag tgcaccacgc
     8761 aggtctggcc gcgcttgggg aaggtgcgcc cgtctcctgg ggagatagtc tccacctgca
     8821 ctcctctaga agccatgcgg ccgctgggcc cagagcgctg gctggggtcc ttaggcttgc
     8881 tcttgctact ccccatgggc ccggatccaa gtataagaca aaagtgttgt ggaattgctc
     8941 caggcgatct gacggttcac taaacgagct ctgcttttat aggcgcccac cgtacacgcc
     9001 tacctcgaca tacgttctct atcactgata gggagtaaac tggatatacg ttctctatca
     9061 ctgataggga gtaaactgta gatacgttct ctatcactga tagggagtaa actggtcata
     9121 cgttctctat cactgatagg gagtaaactc cttatacgtt ctctatcact gatagggagt
     9181 aaagtctgca tacgttctct atcactgata gggagtaaac tcttcatacg ttctctatca
     9241 ctgataggga gtaaactcga gtgaagacga aagggcctca tcgataaact ggatctctgc
     9301 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
     9361 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     9421 ccccttttct tttaaaggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
     9481 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
     9541 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     9601 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     9661 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     9721 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     9781 atta
//
