LOCUS       sequence-46060-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Saini DK, Kalyanaraman V, Chisari M, Gautam N
  TITLE     A family of G protein betagamma subunits translocate reversibly from
            the plasma membrane to endomembranes on receptor activation.
  JOURNAL   J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20.
  PUBMED    17581822
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-17"; volume: "282(33)"; pages: "24099-108. 
            Epub 2007 Jun 20"
COMMENT     Sequence Label: YFP Gamma 1
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(263..280)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(290..1003)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(290..1003)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(330..350)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     945..973
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctagactcga gcggccgcca ctgtgctgga tcgggaattc ttatgaaatc acacagcctc
       61 ctttgagctc cttgaaggga tttttgtcct ctgggatacc ctttactaat ggatcctccc
      121 cagatctttc ttcaacataa tccctgaatt cttcacaaca tttggacacc agcattcttt
      181 ccagcgtcac ttctttcttg agctggtcga cttccatctt caatttgtcc ttttctgtca
      241 ggtcctcaat attgatcact gggtgatggt gatggtgatg catactagtt ttgtatagtt
      301 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      361 tcttttcgtt gggatctttc gaaagcttag attgatagga caggtaatgg ttgtctggta
      421 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      481 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
      541 ctgccatgat gtatacattg tgtgagttat agttgtactc caatttgtgt ccaagaatgt
      601 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      661 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      721 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
      781 ggtatcttgc aaagcacata agaccataac cgaaagtagt gacaagtgtt ggccatggaa
      841 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      901 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
      961 gaattgggac aactccagtg aaaagttctt ctcctttact catggatccg agctcggtac
     1021 caagctt
//
