LOCUS       sequence-460603-        5941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2135m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific brain cell types.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14760 - pAAV-AiE2135m-minBG-iCre-BGHpA (Ali...
FEATURES             Location/Qualifiers
     source          1..5941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             1886..1906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2975..3086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3204..3344
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3204..3333
                     /label=AAV2 ITR
     rep_origin      3419..3874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3506..3525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3716..3737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3891..3910)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4010..4032
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4070..4088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4156..4260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4261..4329,4330..5121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4479..4498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5292..5880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5781..5800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gccggcgtcg ggaacatggg
      181 ggtcaagtga gagagccagg tgtctccagc actcagcatg atatctgaca caaatcagac
      241 ccctcacttt taacactgta ccctaagact tggggcacag gccagggact ttccgttcta
      301 ggctctctgg ctcctgcttc ttggattctg atctccggtc ttatcgactt catttccaaa
      361 ttagaggttt tttggaaccc aggggctacc aggccaacct ggaagtccga ctgcacatgc
      421 tgtatccgtg acagagttac agagaacagg gactgccctg agtgggacag gccgaagagg
      481 gactgccaga gaggtacaga ccccaccccc acctttgtaa gcctgattgc ctacccaggc
      541 caccacaact ccatccagct ctgtccccgc ctttgttcct gagtctccaa ggcccccttt
      601 cctgccaaac aaagccccca gccacccgac tgccgcccaa caggcacctg gggcctctgg
      661 gcctgggaat caaagcctgt tttgcaaagg aggcccagtg tttggagaac acccatcccc
      721 tgctgcccgg tccttcaaag aaccggcatt gttctgcgct gagaaggcgc ctcttgacgc
      781 gctgggcagg gtcggcttga cgctggtgga cctctgatct caggccaggc ccccagccac
      841 ttggggggag ggtggactta ggggccaaag agggagcaac ctgtccttgg ggcactgtgg
      901 tccttcctca ttctctgccc acccgagttc ccatcaccga gttcattgag acccaggctc
      961 ttctacctgg tgatctggac aaggcaggcc tcaggaggca ccaggacaag agatgagata
     1021 gcgggcagga ttttagtgac cataacaggt acgtcagaga ctgcaaacgt attaggcctc
     1081 agtggcatgt aactgtgatt ctagtacttg ggatgctgag gcaggagaat catgagtttg
     1141 aagttaaccg gatcttcaag gcaagaccct gtctctaaat catagatcac aggggagtgt
     1201 gtgtttgtgt gtgtgtgtgt gcgtgcgtgc gcgcatgcgc gtgtacacgt gcgtgtgcat
     1261 atgtatgcat gttctcattt tacagctcac agaggctaga gaggaaactg ggcccagagc
     1321 ccggttcaag ctgtgaactg acaggccccg ttggctcttt tatttgttga agaaatattt
     1381 cccaattact ttcttgtcca ttgtggattc cactgcaagc taacattagt aaaggcattt
     1441 aaataaaagt ctgcccagac acaaactaag gccacagaac tggctgtggt gcccaggcgg
     1501 gtcgtgagga gcccaaataa cactcaaaag ccacctttat ttgtccttcc cctctgccag
     1561 tcacagcatc atgagcttct ggggtggtgt gtgtgtgggg ggggtgttag tctcactcct
     1621 gtggcactgc agaagtctct caaggcacag agagagggca agcaatgtgt tcaaagtcac
     1681 ccagaaactg aggttagatt ggagcggaga gcctcatctc aggagctcat tctgtccggt
     1741 cctcccctca gatcacactt ctgatcaagt ggcacggcag accttcgccg gcggagctcg
     1801 ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc tgggatccag
     1861 atctttcgaa gctagcacca tggtgcccaa gaagaagagg aaagtctcca acctgctgac
     1921 tgtgcaccaa aacctgcctg ccctccctgt ggatgccacc tctgatgaag tcaggaagaa
     1981 cctgatggac atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct
     2041 gtctgtgtgc agatcctggg ctgcctggtg caagctgaac aacaggaaat ggttccctgc
     2101 tgaacctgag gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa
     2161 gaccatccaa cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg
     2221 cccttctgac tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga
     2281 tgctggggag agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag
     2341 atccctgatg gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat
     2401 tgcctacaac accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc
     2461 ccgcaccgat ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac
     2521 agctggtgtg gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc
     2581 tgtgtctggt gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg
     2641 tgtggctgcc ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga
     2701 ggccacccac cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg
     2761 gtctggccac tctgccagag tgggtgctgc cagggacatg gccagggctg gtgtgtccat
     2821 ccctgaaatc atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag
     2881 aaacctggac tctgagactg gggccatggt gaggctgctc gaggatgggg actaatgagg
     2941 cgcgccgaat tcaagcttct cgagagatct tcgactgtgc cttctagttg ccagccatct
     3001 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3061 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3121 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgagatc
     3181 tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3241 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3301 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3361 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3481 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3601 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3721 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     3781 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3841 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     3901 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3961 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4021 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4081 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4141 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4201 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4261 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4321 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4381 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4441 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4501 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4561 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4621 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4681 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4741 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4801 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4861 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4921 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4981 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5041 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5101 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5161 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5221 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5281 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5341 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5401 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5461 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5521 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5581 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5641 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5701 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5761 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5821 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5881 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5941 t
//
