LOCUS       sequence-460632-        6217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid carries csgA and csgG,E,F genes of curli operon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Koksaldi IC, Avci E, Kose S, Ozkul G, Kehribar ES, Safak Seker UO
  TITLE     Genetically engineered bacterial biofilm materials enhances portable
            whole cell sensing.
  JOURNAL   Biosens Bioelectron. 2024 Aug 10;264:116644. doi: 
            10.1016/j.bios.2024.116644.
  PUBMED    39137519
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosens 
            Bioelectron."; date: "2024-08-10"; volume: "264:116644. doi"; pages:
            " 10.1016/j.bios.2024.116644"
COMMENT     Sequence Label: pZa pBAD csgA CBDcex pLac Ara csgGEF
FEATURES             Location/Qualifiers
     source          1..6217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..88
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(155..257)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      294..321
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(404..1282)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWAYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      complement(1564..1650)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3433..3449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3463..3482
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             3823..3834
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4306..4323
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4683..4769
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4804..4821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4938..5483)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5018..5037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5597..5691)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5712..6217,1..154))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
       61 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
      121 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
      181 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
      241 ctcttacgtg ccgatcaacg tctcattttc gccagatatc gacgtctcac actggctcac
      301 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
      361 aatccggctt ttttattatt tcctgcaggg cggccgccat atgttatgac aacttgacgg
      421 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
      481 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
      541 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
      601 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
      661 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
      721 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
      781 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
      841 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
      901 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
      961 gccattcatg ccagtaggcg cgcggacgaa agtaagccca ctggtgatac cattcgcgag
     1021 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     1081 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     1141 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     1201 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     1261 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     1321 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     1381 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     1441 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     1501 gcgtcacact ttgctatgcc ataattgctc agcggtggca gcagcctagg tctagggcgg
     1561 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     1621 gtctttcgac tgagcctttc gttttatttg atgcctctag attaattaat taagcggccg
     1681 catcgatcgg gccctgaggc ctgcagggat cttagaattc atcatgcgcc aaatcgcccg
     1741 tacttaataa cgcctgattt atctggcgac gatttagtgc ttcttcagtt tgaatcagtg
     1801 caaagacgac agttttctcg aagtctcttt tcaacggaaa taaaaaagtc tggaaaataa
     1861 cgtcctgatt gaccgttata gtgatccagc ttccccatcg tgcactgggc ctttcattaa
     1921 tcgttaagtt acccgtatag tcactttccc atttatcact aaaggctcgg taaaaatcat
     1981 ggccaataga tgaaacagta tggtcagtta gcaatcccgg gacttctacc tcaacggcgt
     2041 gaagattccc tgcggcgaac agaaattctg ccgccacaat ccagcgtaaa taacgtttca
     2101 tctcctttgt acattaaaaa tcggttgagt tattttgtaa acccgaaacc tggatggtcg
     2161 aggtttgtcc ggttttacga tctgtcacgt tcaactgcaa ttgaccatcg cggttggcaa
     2221 tatcgacaat ataatcgttg gtcaccatgc ggcccggttt accggtatta atattcgaca
     2281 gtagcccacc taaaatttgt gactggatgg cctgagtaaa gttatctaac gctgagggtg
     2341 tttcaatacc aaagtcatcg ttatagctcg gatctttata agagttttgg gcctgagcgc
     2401 tatttaataa aaaagcgcca ttatttgggt taccaccaaa gtttggatta cggaactgga
     2461 aagtcatggt tccagcccaa cttaatggcg aaataagcat gagtagaact actgcatgtt
     2521 tgacacgcat ctccttgtcg acgatccaag ctttcaggat tccggtggaa ccgacatatg
     2581 gcggtatttc accagaatgt cattctgccg ttctgcttta ttttgcaaat cccacagacc
     2641 acggtcgata ccatcattaa tcaggaaaat gacccctgtt tcgatagccg acatcaggca
     2701 cagcataaca ggttcgttcg aggtgtaacc cacttcccct tcaagcaagc gctggtagtc
     2761 aataaagcgg aaaaccccgg cctgaacttc ataggaaagt atcgtcttac tggtgttcac
     2821 cgaagaaagg atctcgccgg tactcacatt gacgacgcgc aggttcacgg caatctgatc
     2881 gagctggtat tgcgtgtcgg caccgatgcc aaaatatctt gccccaaccc cgccagattt
     2941 gacgttgctt tcataaccga taatcgaacc ttcaaccatg atatttgccg ccgttaaaga
     3001 ttgcagcggg attcggttat taatggcaac cgtgccgttt tcttgtgccg cacgaataat
     3061 cttgcgctcg ttaagcaggt tttgtaagcc ctggcgctcc agcggtataa accagcgaga
     3121 atctttcagt gccgtgacca gcattgccgt ggcgctttgc ggaacagcag tggagaagtt
     3181 acttgccggg tagggtttaa attgcccggt ttcgtcctga atgttgtata ccgaaacaaa
     3241 gattttaccc gtcggcgctg gcagatgggt caaatctttg tagctctgag cacgaggcat
     3301 taatgtcggt ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac
     3361 ggcaaccaaa agaaataagc gctgcatggt accctccttt ctagattttc tcaagcctag
     3421 gtctgtgtga aattgttatc cgctcacaat tgaatctatc ataattgtga gcgctcacaa
     3481 ttgtaaaggt tagatccgct aatcttatgg ataaaaatgc tatgttcccc ccggggggat
     3541 atcaacagga gtccaagcga ccggtggttg catgtctagc tagctagaac aggactagct
     3601 aatggtttct tagacgtcgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     3661 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tggcattttt atccataaga
     3721 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt
     3781 tttgggctag ctactagagt cacacaggaa acctactaga ttaaagagga gaaaggtacc
     3841 atgaaacttt taaaagtagc agcaattgca gcaatcgtat tctccggtag cgctctggca
     3901 ggtgttgttc ctcagtacgg cggcggcggt aaccacggtg gtggcggtaa taatagcggc
     3961 ccaaattctg agctgaacat ttaccagtac ggtggcggta actctgcact tgctctgcaa
     4021 actgatgccc gtaactctga cttgactatt acccagcatg gcggcggtaa tggtgcagat
     4081 gttggtcagg gctcagatga cagctcaatc gatctgaccc aacgtggctt cggtaacagc
     4141 gctactcttg atcagtggaa cggcaaaaat tctgaaatga cggttaaaca gttcggtggt
     4201 ggcaacggtg ctgcagttga ccagactgca tctaactcct ccgtcaacgt gactcaggtt
     4261 ggctttggta acaacgcgac cgctcatcag tacctcgagg gtagccacca ccaccaccac
     4321 cacggatccc cgacgtccgg tccggccggg tgccaggtgc tgtggggcgt caaccagtgg
     4381 aacaccggct tcaccgcgaa cgtcaccgtg aagaacacgt cctccgctcc ggtagacggc
     4441 tggacgctca cgttcagctt cccgtccggc cagcaggtca cccaggcgtg gagctcgacg
     4501 gtcacgcagt ccggctcggc cgtgacggtc cgcaacgccc cgtggaacgg ctcgatcccg
     4561 gcgggcggca ccgcgcagtt cggcttcaac ggctcgcaca cgggcaccaa cgccgcgccg
     4621 acggcgttct cgctcaacgg cacgccctgc acggtcggct aataactcga gacgcgtggc
     4681 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     4741 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctaggga tatattccgc
     4801 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     4861 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     4921 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     4981 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5041 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     5101 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     5161 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     5221 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     5281 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     5341 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     5401 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     5461 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     5521 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     5581 tgttactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     5641 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     5701 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     5761 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     5821 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     5881 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     5941 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     6001 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     6061 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     6121 atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg
     6181 gcaagaatgt gaataaaggc cggataaaac ttgtgct
//
