LOCUS       sequence-46065-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Saini DK, Kalyanaraman V, Chisari M, Gautam N
  TITLE     A family of G protein betagamma subunits translocate reversibly from
            the plasma membrane to endomembranes on receptor activation.
  JOURNAL   J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20.
  PUBMED    17581822
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-17"; volume: "282(33)"; pages: "24099-108. 
            Epub 2007 Jun 20"
COMMENT     Sequence Label: YFP Gamma 7
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(266..283)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(333..353)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgcgatcacc actttgtaca agaaagctgg gtctagatat ctcgagtgcg gccgcgaatt
       61 cctataggat aatacaaggc ttcttgtcct taaaggggtt ctcggaggcg gggactccca
      121 ccagcagggg gtcgttccgg gcgtgctgct cacagtagct catgagttca gaggaggctt
      181 tggagacctt aatgcgctcg atcccagctt cgatgcggag ctgctccacc agcttccgag
      241 cctgggctat gttgttagtg gctgagtgat ggtgatggtg atgcatacta gttttgtata
      301 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
      361 ctctcttttc gttgggatct ttcgaaagct tagattgata ggacaggtaa tggttgtctg
      421 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      481 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
      541 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ctccaatttg tgtccaagaa
      601 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      661 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
      721 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat
      781 ctgggtatct tgcaaagcac ataagaccat aaccgaaagt agtgacaagt gttggccatg
      841 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
      901 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa
      961 caagaattgg gac
//
