LOCUS       sequence-46067-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Saini DK, Kalyanaraman V, Chisari M, Gautam N
  TITLE     A family of G protein betagamma subunits translocate reversibly from
            the plasma membrane to endomembranes on receptor activation.
  JOURNAL   J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20.
  PUBMED    17581822
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-17"; volume: "282(33)"; pages: "24099-108. 
            Epub 2007 Jun 20"
COMMENT     Sequence Label: YFP Gamma 9
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(252..269)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(319..339)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctagactcga gcggccgcca ctgtgctgga tatctgcaga attctcagct tattatacaa
       61 ccgccttttt ccttgaaggg attcttgtcc tcagggatgc ctttaagaag agggtcgttt
      121 cctgcttcag cctccacata atccttgatt tcctttcctg ttttggaaat cagagctctt
      181 gggttcttca cttccttctt cagctgctct acctccatct ttaagagttc cttctcgctg
      241 agctcttggg cgtgatggtg atggtgatgc atactagttt tgtatagttc atccatgcca
      301 tgtgtaatcc cagcagctgt tacaaactca agaaggacca tgtggtctct cttttcgttg
      361 ggatctttcg aaagcttaga ttgataggac aggtaatggt tgtctggtaa aaggacaggg
      421 ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgct tccatcttca
      481 atgttgtgtc taattttgaa gttaactttg attccattct tttgtttgtc tgccatgatg
      541 tatacattgt gtgagttata gttgtactcc aatttgtgtc caagaatgtt tccatcttct
      601 ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg tatcaccttc aaacttgact
      661 tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc ctgtacataa
      721 ccttcgggca tggcactctt gaaaaagtca tgctgtttca tatgatctgg gtatcttgca
      781 aagcacataa gaccataacc gaaagtagtg acaagtgttg gccatgggaa cagggtagtt
      841 ttccagta
//
