LOCUS       sequence-460678-        3510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gateway compatible 5' entry clone containing an MCS upstream of 
            the murine c-fos minimal promotor and b-globin intron. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: p5E-MCS-c-Fos-b-globin (JDW 1237)
FEATURES             Location/Qualifiers
     source          1..3510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          890..1462
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    1556..1679
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1751..1770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1752..1770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1775..1791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1775..1791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1893..1912)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1904..2713
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1971..1990)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2860..3448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3349..3368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtc tagaggtacc cctcgagaag cttactagta
      721 gatctcatat gttaattaag ctagcgatat cgtcgaccca gtgacgtagg aagtccatcc
      781 attcacagcg cttctataaa ggcgccagct gaggcgccta ctactccaac cgcgactgca
      841 gcgagcaact gagctcggat ccgtttaaac cgatcctgag aacttcaggg tgagtttggg
      901 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
      961 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
     1021 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
     1081 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
     1141 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
     1201 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
     1261 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
     1321 aaacaactac accctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
     1381 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
     1441 tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc tgtctcatca
     1501 ttttggcaaa gaattcctcg acaccggtat cgatacgcgt tacgtagcgg ccgctcaagt
     1561 ttgtacaaaa aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     1621 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatgca gtcactatga
     1681 accaactact tagatggtat tagtgacctg tagaattcga gctctagagc tgcagggcgg
     1741 ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1801 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     1861 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1921 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1981 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2041 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     2101 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2161 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2221 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     2281 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     2341 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     2401 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     2461 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     2521 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     2581 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     2641 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     2701 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2761 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3001 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3241 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3481 ggccttttgc tggccttttg ctcacatgtt
//
