LOCUS       sequence-46069-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Saini DK, Kalyanaraman V, Chisari M, Gautam N
  TITLE     A family of G protein betagamma subunits translocate reversibly from
            the plasma membrane to endomembranes on receptor activation.
  JOURNAL   J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20.
  PUBMED    17581822
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-17"; volume: "282(33)"; pages: "24099-108. 
            Epub 2007 Jun 20"
COMMENT     Sequence Label: YFP Gamma 13
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(263..280)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(330..350)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgcgatcacc actttgtaca agaaagctgg gtctagatat ctcgagtgcg gccgcgaatt
       61 ctcacaggat ggtgcatttg cccttttcca cccatgggtt gttcttcatc aggtcggggt
      121 tcaggaaggg gtccttgggg atcccgtcct cgatccactt cagcagctcg gggatggtct
      181 tggacgccat ctcccgctgg aaggccagct ggtacttaag gctctccacc tctttcttca
      241 tctgtggcac gtcccactcc tcgtgatggt gatggtgatg catactagtt ttgtatagtt
      301 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      361 tcttttcgtt gggatctttc gaaagcttag attgatagga caggtaatgg ttgtctggta
      421 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      481 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
      541 ctgccatgat gtatacattg tgtgagttat agttgtactc caatttgtgt ccaagaatgt
      601 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      661 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      721 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
      781 ggtatcttgc aaagcacata agaccataac cgaaagtagt gacaagtgtt ggccatggaa
      841 caggtagttt tccagtagtg caaataaatt taaagggtaa gttttccgta tgttgcatca
      901 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcacca
//
