LOCUS       sequence-460692-        4649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ420 - Peft-3::GFP::H2B (ubiquitous)
FEATURES             Location/Qualifiers
     source          1..4649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..600
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    609..633
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(666..694)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1442..1462
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(1923..1947)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1952..2275
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(2279..2299)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(2436..3024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2516..2535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3195..3986,3987..4055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3818..3837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4056..4160)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4228..4246
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4284..4306)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    4629..4649
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
       61 tgaatttttc ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata
      121 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
      181 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
      241 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
      301 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
      361 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
      421 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
      481 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
      541 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
      601 ctttgctcac aagtttgtac aaaaaagcag gctccatgag taaaggagaa gaacttttca
      661 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      721 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      781 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
      841 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
      901 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
      961 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
     1021 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
     1081 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1141 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1201 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
     1261 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1321 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1381 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1441 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1501 aatgcccggg ggatcggtgg agctccaccg gtggcggccg ctctagaact agtccaccaa
     1561 agccatctgc caagggagcc aagaaggccg ccaagaccgt cgttgccaag ccaaaggacg
     1621 gaaagaagag acgtcatgcc cgcaaggaat cgtactccgt ctacatctac cgtgttctca
     1681 agcaagttca cccagacacc ggagtctcct ccaaggccat gtctatcatg aactccttcg
     1741 tcaacgatgt attcgaacgc atcgcttcgg aagcttcccg tcttgctcat tacaacaaac
     1801 gctcaacgat ctcatcccgc gaaattcaaa ccgctgtccg tttgattctc ccaggagaac
     1861 ttgccaagca cgccgtgtct gagggaacca aggccgtcac caagtacact tccagcaagt
     1921 aaacccagct ttcttgtaca aagtgggata aatgcaaaat cctttcaagc attcccttct
     1981 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     2041 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
     2101 tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa ttccattttg
     2161 aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt
     2221 cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa aagtctcaca
     2281 actttattat acatagttga taattcgatg gtatcagctc actcaaaggc ggtaatacgg
     2341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2401 accaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3181 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3301 ttaccatctg accccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3481 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3781 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4081 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4141 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4201 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     4261 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4321 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4381 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgcactgag agtgcaccat
     4441 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     4501 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4561 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggatccgc
     4621 caagctatca actttgtata gaaaagttg
//
