LOCUS       sequence-460765-        9883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses components of the LOOMINA optogenetic transcriptional 
            control system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Muench P, Fiumara M, Southern N, Coda D, Aschenbrenner S, Correia B,
            Graff J, Niopek D, Mathony J
  TITLE     A modular toolbox for the optogenetic deactivation of transcription.
  JOURNAL   Nucleic Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237.
  PUBMED    39676667
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237."
COMMENT     Sequence Label: Zdk3-VP64
FEATURES             Location/Qualifiers
     source          1..9883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..36)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        208..587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        589..787
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     742..762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             805..985
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1032..1157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1650..1883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2068..2112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2410..2527
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2652..3830
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2883..3821
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3778..3798
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3837..3846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3855..3875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4137..4286
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4308..4328
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4381..4969
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4434..4454)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4852..4863)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4972..4988)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4973..4989)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5494..5674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5700..5717)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5706..5930
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6140..6568
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6227..6246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6437..6458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6577..6597)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6582..6911
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6762..6897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6824..6843
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6959..7006
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7025..7399
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7529..7650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7566..7585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7620..7639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7699..7715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7699..7715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7712..7734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7723..7739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7747..7753,7754..7771,7772..7777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7792..7813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7930..7947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8181..8200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8860..9651,9652..9720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9483..9502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9721..9825)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
       61 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      181 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      241 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      301 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      361 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      421 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      481 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      541 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      601 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      661 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      721 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      781 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      961 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1021 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1081 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1141 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1201 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1261 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1321 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1381 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1441 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1501 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1561 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1621 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1681 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1741 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1801 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1861 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1921 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1981 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2041 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2101 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2161 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2221 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2281 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2341 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     2401 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2461 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2521 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta atgcaaagat
     2581 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     2641 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2701 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2761 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2821 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2881 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     2941 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     3001 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     3061 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     3121 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     3181 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     3241 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     3301 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     3361 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     3421 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     3481 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     3541 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     3601 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     3661 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     3721 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     3781 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacggcca
     3841 ccatgggtag cggacctaag aaaaagagga aggtggcggc cgctggaggt tctggaagtg
     3901 gatccatggt ggataacaaa ttcaataaag aagtgctggt ggcccggcag gaaatctatt
     3961 ggctgccaaa cctgaattgg gagcagaagt ttgccttcat cagtagcctg actaatgatc
     4021 catctcagag cgccaatctg ctggccgaag ccaaaaaact gaacggtgcc cagcccccaa
     4081 aaggaggagg tggaagcgga ggcggcggaa gtgcggccgc tggatccgga cgggctgacg
     4141 cattggacga ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg
     4201 acatgcttgg ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc
     4261 ttgatgattt cgacctggac atgctgggtt ctggaagtgg atccagcccc aagaagaaga
     4321 gaaaggtgga ggccagctaa agcgaagtca atcccagaat tcgatatcaa gcttatcgat
     4381 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4441 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4501 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4561 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4621 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4681 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4741 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4801 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4861 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4921 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     4981 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     5041 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     5101 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5161 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     5221 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     5281 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     5341 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     5401 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     5461 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     5521 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5581 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5641 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     5701 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5761 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5821 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5881 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5941 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6001 cgcggcgggg gggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6061 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6121 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6541 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6661 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6721 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6781 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6841 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6901 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     6961 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     7021 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     7081 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     7141 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     7201 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     7261 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     7321 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     7381 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     7441 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7501 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7621 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9841 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccc
//
