LOCUS       sequence-460773-        9084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 
            1-180 necroptosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Hanggi K, Li J, Gangadharan A, Liu X, Celias DP, Osunmakinde O, 
            Keske A, Davis J, Ahmad F, Giron A, Anadon CM, Gardner A, DeNardo 
            DG, Shaw TI, Beg AA, Yu X, Ruffell B
  TITLE     Interleukin-1alpha release during necrotic-like cell death generates
            myeloid-driven immunosuppression that restricts anti-tumor immunity.
  JOURNAL   Cancer Cell. 2024 Nov 13:S1535-6108(24)00402-1. doi: 
            10.1016/j.ccell.2024.10.014.
  PUBMED    39577420
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2024 Nov 13:S1535-6108(24)00402-1. doi: 
            10.1016/j.ccell.2024.10.014."
COMMENT     Sequence Label: pLVX MLKL(1-180)-2A-mCherry-puro
FEATURES             Location/Qualifiers
     source          1..9084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    524..649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             696..1329
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1497..1631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1521..1540)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1575..1594
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1679..1783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1784..1852,1853..2644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2002..2021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2815..3403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3304..3323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3557..3574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3691..3712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3727..3732,3733..3750,3751..3757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3765..3781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3770..3792
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3789..3805
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3789..3805
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             3933..4566
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4773..5361
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4879..4890
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5288..5308
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5378..5977)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5461..5481)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5958..5977
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5998..6497)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6046..6065)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6439..6462
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(6519..7226)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6794..6813)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7063..7081
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7227..7280)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(7859..8208)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(7896..7920)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7966..7984
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8002..8020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8038..8056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8074..8092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8110..8128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8146..8164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8182..8200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    8271..8386
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8649..8693)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(8878..9084,1..27)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
       61 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
      121 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
      181 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
      241 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
      301 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
      361 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
      421 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
      481 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
      541 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
      601 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
      661 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
      721 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
      781 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
      841 ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt
      901 atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa
      961 agtccccagc ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg
     1021 gatgcagctc tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca
     1081 cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct
     1141 ctcctttatt ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc
     1201 caaaggtcag tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt
     1261 agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta
     1321 gcccttccaa ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa
     1381 aataattcag aggaatcaca ggtttagagt aaatgaaacc acaggtaatt ggcagtggta
     1441 atagggtatg gggtgggaag tttgggatga ttttggttag cttgagttat ccagttgatc
     1501 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1561 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1621 ataaacaagt tgatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
     1681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1801 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1861 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1921 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1981 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2041 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2101 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2161 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2221 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2281 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2341 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2401 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2461 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2521 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2581 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2641 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2701 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2761 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2941 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3601 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3661 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3721 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3781 atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg
     3841 taaacgctag caatgtcaag gcctctcact ctctgatatt catttctttg caagttataa
     3901 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     3961 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     4021 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     4081 ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata
     4141 tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt
     4201 ccccagcgga aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat
     4261 gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt
     4321 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc
     4381 ctttattggc ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa
     4441 aggtcagtgg atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc
     4501 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc
     4561 cttccagtcc cctcttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     4621 ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt
     4681 ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt
     4741 tttctaggtc tcgactgcag aattaattcc aggcggggag gcggcccaaa gggagatccg
     4801 actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg
     4861 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag
     4921 aatccaggtg gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac
     4981 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc
     5041 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca
     5101 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc
     5161 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa
     5221 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc
     5281 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat
     5341 tttgtaatcc agaggttgat tgttccagac gcgcccgtca ggcaccgggc ttgcgggtca
     5401 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     5461 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     5521 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     5581 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     5641 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     5701 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     5761 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     5821 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     5881 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     5941 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagcttgggc tgcaggtcga
     6001 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     6061 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     6121 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     6181 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     6241 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     6301 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     6361 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     6421 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     6481 aagcgcctcc cctacccggt atcagtcagt cagaattcct tgtacagctc gtccatgccg
     6541 ccggtggagt ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg
     6601 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
     6661 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
     6721 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
     6781 tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
     6841 agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc
     6901 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
     6961 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
     7021 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
     7081 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     7141 ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg
     7201 ttatcctcct cgcccttgct caccataggg ccgggattct cctccacgtc accgcatgtt
     7261 agaagacttc ctctgccctc gaattccata tgacgcgtat cttgtgggat ctcctgtgtg
     7321 ggttttagtg agcattgctt cagggttttg ttgatttctt ccacgctaat ttgcaactgc
     7381 atcaggataa ctttcatatt ttcatttccg tcttcctctg catcctgtcg atcttcctgc
     7441 tgccaggatg ctggctggct gacatctgaa acggtattcc aatgataaac ctgaagcagc
     7501 agcaacagct cctcccagac gtctctcagc ttctcattca cttcatggaa gaggatctta
     7561 tcattgccca cactcacaaa cttccaaata tgggacttct tgctgaactt ttctatctgc
     7621 tggttagcct ccttcaggac ttcatcaaaa cggcccaggg cagcagtaat gtcatcgggc
     7681 aggttcttct ttccttgggc ctggagtctc tggagaggct gtagcaggcc gtgcacacgg
     7741 tttcctagac gctggcattg tttccggcag tatttcatct tttcacactg ttcatagatg
     7801 agctggccta acttgatgat ctgtcccaat ttatccatgc ggccgcgggc ccggatccaa
     7861 gtataagaca aaagtgttgt ggaattgctc caggcgatct gacggttcac taaacgagct
     7921 ctgcttttat aggcgcccac cgtacacgcc tacctcgaca tacgttctct atcactgata
     7981 gggagtaaac tggatatacg ttctctatca ctgataggga gtaaactgta gatacgttct
     8041 ctatcactga tagggagtaa actggtcata cgttctctat cactgatagg gagtaaactc
     8101 cttatacgtt ctctatcact gatagggagt aaagtctgca tacgttctct atcactgata
     8161 gggagtaaac tcttcatacg ttctctatca ctgataggga gtaaactcga gtgaagacga
     8221 aagggcctca tcgataaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
     8281 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     8341 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaggcg ataccgtcga
     8401 gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt
     8461 ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
     8521 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
     8581 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
     8641 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
     8701 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
     8761 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
     8821 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
     8881 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
     8941 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
     9001 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
     9061 gcgcccatag tgcttcctgc tgct
//
