LOCUS       sequence-460777-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the ZNF671 transcription factor
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0045
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2908..3496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2961..2981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3379..3390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3568..3801
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3879..4000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3916..3935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3970..3989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4040..4175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4195..4214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4196..4214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4224..4241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4224..4240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4233..4255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4382..4837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4469..4488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4679..4700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4863..4967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4968..5036,5037..5828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5186..5205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5999..6587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6488..6507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6741..6758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6875..6896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6911..6916,6917..6934,6935..6941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6949..6965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6954..6976
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6973..6989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6973..6989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7008..7028
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7010..7028
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7056..7282
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7283..7463
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7510..7635
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8128..8361
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8546..8590
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8821..9031
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8843..9039
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8890..9025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9071..9673
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9071..9090)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9567..9587
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9716..9833
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 gtccccagtg tcccgagacg cgtctgatgc tctgcaggga cggaagtgcc tgcgtccccg
     1261 atcgagacgc ctgccgctcc cggcagctgt ccgcgcccac ggtcctatgg cggagctaac
     1321 ggactccgcg cggggctgtg tggtctttga ggatgtgttt gtatacttct ctcgggaaga
     1381 atgggagctt cttgatgatg ctcagagact tttgtaccat gatgtgatgc tggagaactt
     1441 tgcactttta gcctcactgg gaattgcatt ttccagatca cgtgcagtca tgaaactaga
     1501 gcgaggagaa gagccctggg tgtatgacca ggtggatatg acttcagcca cagaaagaga
     1561 ggcccagagg ggacttagac ctggttgttg gcatggagtg gaggatgaag aggtatcttc
     1621 tgagcagagc atttttgtag caggagtgtc agaggtcagg actctcatgg cagagctgga
     1681 gtctcaccca tgtgacatat gtggcccaat attgaaagat accttacacc tggctaaata
     1741 ccatggggga aaagccaggc agaaaccata cttgtgtggg gcatgtggaa agcaattctg
     1801 gttcagtaca gactttgacc agcaccagaa ccagcccaat ggagggaaac ttttcccaag
     1861 gaaggagggc agagactctg tgaaaagctg cagagtccat gtgccagaga agaccctcac
     1921 atgtgggaaa ggtaggagag acttttcagc cacatctggc cttcttcagc atcaggcctc
     1981 tctcagcagc atgaagcccc acaagagcac taagcttgtg agtggctttc tcatgggaca
     2041 gaggtatcac aggtgtggtg aatgtgggaa agccttcacc cgcaaagaca cacttgctcg
     2101 gcatcagaga atccacactg gagaaaggcc ttatgagtgt aacgaatgtg ggaaattctt
     2161 cagccaaagc tatgacctct ttaaacacca gacagttcac actggagaaa ggccatacga
     2221 gtgcagcgaa tgtgggaaat tctttagaca aatctccggc ctgattgagc acaggcgagt
     2281 tcacacgggt gaaagactct atcagtgtgg caaatgtggg aaatttttta gcagtaagtc
     2341 taatctcatt cgacaccagg aagttcacac aggagccagg ccttatgtat gcagcgaatg
     2401 tgggaaagag ttcagtcgga aacacacact tgttctgcac caacgaactc acactggaga
     2461 aaggccttat gagtgcagtg aatgtgggaa ggcctttagc caaagctccc accttaatgt
     2521 acactggaga attcacagca gtgattatga gtgtagcaga tgtggtaaag ctttcagctg
     2581 catctccaaa ctcattcagc accagaaagt tcactctgga gaaaagcctt atgagtgcag
     2641 caagtgcggg aaagccttca ctcaaagacc caacctcatc aggcactgga aagtccacac
     2701 tggggaaagg ccttatgtgt gtagtgagtg cgggagagaa ttcatccgga aacagacact
     2761 tgttctgcac cagagggttc atgctggaga aaagctttag taatgaacta gtccgtaact
     2821 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaac tgagcgttca
     2881 gtcttctcca acacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2941 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3001 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3061 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3121 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3181 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3241 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3301 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3361 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3421 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3481 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3541 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3601 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3661 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3721 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3781 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     3841 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3901 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3961 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4081 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4141 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4201 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4261 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4321 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4381 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4801 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6841 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6901 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6961 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7021 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7081 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7141 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7201 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7261 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7321 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7381 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7441 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7501 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7561 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7621 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7681 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7741 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7801 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7861 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7921 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7981 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8041 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8101 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8161 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8221 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8281 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8341 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8401 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8461 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8521 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8581 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8641 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8701 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8761 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8821 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     8881 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8941 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9001 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     9061 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9121 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9181 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9241 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9301 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9361 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9421 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9481 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9541 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9601 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9661 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9721 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9781 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9841 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
     9901 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     9961 agtgggaatt
//
