LOCUS       sequence-46078-e       12880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12880)
  TITLE     NLdDIS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pfNL43-dE-EGFP
FEATURES             Location/Qualifiers
     source          1..12880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             69..326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             482..1984
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1777..4788
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDGLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4473..4590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      6060..6069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6066..6068,6069..6071,6072..6782)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6066..6068,6069..6071,6072..6782)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6111..6132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6372..6391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6719..6740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7304..7537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7722..7766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8060..8086
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     LTR             8621..9254
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10243..10260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10414..11002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10494..10513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11173..11964,11965..12033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11796..11815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12034..12138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12206..12224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12262..12284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12384..12403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccggagtact acaaagactg ctgacatcga gctttctaca agggactttc cgctggggac
       61 tttccaggga ggtgtggcct gggcgggact ggggagtggc gagccctcag atgctacata
      121 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      241 caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      301 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta
      361 aagccagagg agatctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      421 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      481 gatgggtgcg agagcgtcgg tattaagcgg gggagaatta gataaatggg aaaaaattcg
      541 gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg caagcaggga
      601 gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct gtagacaaat
      661 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
      721 tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc
      781 cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc
      841 tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga acctccaggg
      901 gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa aagtagtaga
      961 agagaaggct ttcagcccag aagtaatacc catgttttca gcattatcag aaggagccac
     1021 cccacaagat ttaaatacca tgctaaacac agtgggggga catcaagcag ccatgcaaat
     1081 gttaaaagag accatcaatg aggaagctgc agaatgggat agattgcatc cagtgcatgc
     1141 agggcctatt gcaccaggcc agatgagaga accaagggga agtgacatag caggaactac
     1201 tagtaccctt caggaacaaa taggatggat gacacataat ccacctatcc cagtaggaga
     1261 aatctataaa agatggataa tcctgggatt aaataaaata gtaagaatgt atagccctac
     1321 cagcattctg gacataagac aaggaccaaa ggaacccttt agagactatg tagaccgatt
     1381 ctataaaact ctaagagccg agcaagcttc acaagaggta aaaaattgga tgacagaaac
     1441 cttgttggtc caaaatgcga acccagattg taagactatt ttaaaagcat tgggaccagg
     1501 agcgacacta gaagaaatga tgacagcatg tcagggagtg gggggacccg gccataaagc
     1561 aagagttttg gctgaagcaa tgagccaagt aacaaatcca gctaccataa tgatacagaa
     1621 aggcaatttt aggaaccaaa gaaagactgt taagtgtttc aattgtggca aagaagggca
     1681 catagccaaa aattgcaggg cccctaggaa aaagggctgt tggaaatgtg gaaaggaagg
     1741 acaccaaatg aaagattgta ctgagagaca ggctaatttt ttagggaaga tctggccttc
     1801 ccacaaggga aggccaggga attttcttca gagcagacca gagccaacag ccccaccaga
     1861 agagagcttc aggtttgggg aagagacaac aactccctct cagaagcagg agccgataga
     1921 caaggaactg tatcctttag cttccctcag atcactcttt ggcagcgacc cctcgtcaca
     1981 ataaagatag gggggcaatt aaaggaagct ctattagata caggagcaga tgatacagta
     2041 ttagaagaaa tgaatttgcc aggaagatgg aaaccaaaaa tgataggggg aattggaggt
     2101 tttatcaaag taggacagta tgatcagata ctcatagaaa tctgcggaca taaagctata
     2161 ggtacagtat tagtaggacc tacacctgtc aacataattg gaagaaatct gttgactcag
     2221 attggctgca ctttaaattt tcccattagt cctattgaga ctgtaccagt aaaattaaag
     2281 ccaggaatgg atggcccaaa agttaaacaa tggccattga cagaagaaaa aataaaagca
     2341 ttagtagaaa tttgtacaga aatggaaaag gaaggaaaaa tttcaaaaat tgggcctgaa
     2401 aatccataca atactccagt atttgccata aagaaaaaag acagtactaa atggagaaaa
     2461 ttagtagatt tcagagaact taataagaga actcaagatt tctgggaagt tcaattagga
     2521 ataccacatc ctgcagggtt aaaacagaaa aaatcagtaa cagtactgga tgtgggcgat
     2581 gcatattttt cagttccctt agataaagac ttcaggaagt atactgcatt taccatacct
     2641 agtataaaca atgagacacc agggattaga tatcagtaca atgtgcttcc acagggatgg
     2701 aaaggatcac cagcaatatt ccagtgtagc atgacaaaaa tcttagagcc ttttagaaaa
     2761 caaaatccag acatagtcat ctatcaatac atggatgatt tgtatgtagg atctgactta
     2821 gaaatagggc agcatagaac aaaaatagag gaactgagac aacatctgtt gaggtgggga
     2881 tttaccacac cagacaaaaa acatcagaaa gaacctccat tcctttggat gggttatgaa
     2941 ctccatcctg ataaatggac agtacagcct atagtgctgc cagaaaagga cagctggact
     3001 gtcaatgaca tacagaaatt agtgggaaaa ttgaattggg caagtcagat ttatgcaggg
     3061 attaaagtaa ggcaattatg taaacttctt aggggaacca aagcactaac agaagtagta
     3121 ccactaacag aagaagcaga gctagaactg gcagaaaaca gggagattct aaaagaaccg
     3181 gtacatggag tgtattatga cccatcaaaa gacttaatag cagaaataca gaagcagggg
     3241 caaggccaat ggacatatca aatttatcaa gagccattta aaaatctgaa aacaggaaaa
     3301 tatgcaagaa tgaagggtgc ccacactaat gatgtgaaac aattaacaga ggcagtacaa
     3361 aaaatagcca cagaaagcat agtaatatgg ggaaagactc ctaaatttaa attacccata
     3421 caaaaggaaa catgggaagc atggtggaca gagtattggc aagccacctg gattcctgag
     3481 tgggagtttg tcaatacccc tcccttagtg aagttatggt accagttaga gaaagaaccc
     3541 ataataggag cagaaacttt ctatgtagat ggggcagcca atagggaaac taaattagga
     3601 aaagcaggat atgtaactga cagaggaaga caaaaagttg tccccctaac ggacacaaca
     3661 aatcagaaga ctgagttaca agcaattcat ctagctttgc aggattcggg attagaagta
     3721 aacatagtga cagactcaca atatgcattg ggaatcattc aagcacaacc agataagagt
     3781 gaatcagagt tagtcagtca aataatagag cagttaataa aaaaggaaaa agtctacctg
     3841 gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagatgg gttggtcagt
     3901 gctggaatca ggaaagtact atttttagat ggaatagata aggcccaaga agaacatgag
     3961 aaatatcaca gtaattggag agcaatggct agtgatttta acctaccacc tgtagtagca
     4021 aaagaaatag tagccagctg tgataaatgt cagctaaaag gggaagccat gcatggacaa
     4081 gtagactgta gcccaggaat atggcagcta gattgtacac atttagaagg aaaagttatc
     4141 ttggtagcag ttcatgtagc cagtggatat atagaagcag aagtaattcc agcagagaca
     4201 gggcaagaaa cagcatactt cctcttaaaa ttagcaggaa gatggccagt aaaaacagta
     4261 catacagaca atggcagcaa tttcaccagt actacagtta aggccgcctg ttggtgggcg
     4321 gggatcaagc aggaatttgg cattccctac aatccccaaa gtcaaggagt aatagaatct
     4381 atgaataaag aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag
     4441 acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4501 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4561 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4621 gtttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat
     4681 aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa
     4741 cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattaaca catggaaaag
     4801 attagtaaaa caccatatgt atatttcaag gaaagctaag gactggtttt atagacatca
     4861 ctatgaaagt actaatccaa aaataagttc agaagtacac atcccactag gggatgctaa
     4921 attagtaata acaacatatt ggggtctgca tacaggagaa agagactggc atttgggtca
     4981 gggagtctcc atagaatgga ggaaaaagag atatagcaca caagtagacc ctgacctagc
     5041 agaccaacta attcatctgc actattttga ttgtttttca gaatctgcta taagaaatac
     5101 catattagga cgtatagtta gtcctaggtg tgaatatcaa gcaggacata acaaggtagg
     5161 atctctacag tacttggcac tagcagcatt aataaaacca aaacagataa agccaccttt
     5221 gcctagtgtt aggaaactga cagaggacag atggaacaag ccccagaaga ccaagggcca
     5281 cagagggagc catacaatga atggacacta gagcttttag aggaacttaa gagtgaagct
     5341 gttagacatt ttcctaggat atggctccat aacttaggac aacatatcta tgaaacttac
     5401 ggggatactt gggcaggagt ggaagccata ataagaattc tgcaacaact gctgtttatc
     5461 catttcagaa ttgggtgtcg acatagcaga ataggcgtta ctcgacagag gagagcaaga
     5521 aatggagcca gtagatccta gactagagcc ctggaagcat ccaggaagtc agcctaaaac
     5581 tgcttgtacc aattgctatt gtaaaaagtg ttgctttcat tgccaagttt gtttcatgac
     5641 aaaagcctta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa gagctcatca
     5701 gaacagtcag actcatcaag cttctctatc aaagcagtaa gtagtacatg taatgcaacc
     5761 tataatagta gcaatagtag cattagtagt agcaataata atagcaatag ttgtgtggtc
     5821 catagtaatc atagaatata ggaaaatatt aagacaaaga aaaatagaca ggttaattga
     5881 tagactaata gaaagagcag aagacagtgg caatgagagt gaaggagaag tatcagcact
     5941 tgtggagatg ggggtggaaa tggggcacca tgctccttgg gatattgatg atctgtagtg
     6001 ctacagaaaa attgtgggtc acagtctatt atggggtacc taaggcctat ccacctgtcg
     6061 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     6121 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     6181 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     6241 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     6301 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     6361 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     6421 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     6481 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     6541 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     6601 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     6661 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     6721 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     6781 aggacgagct gtaagctagc aaattaagag aacaatttgg aaataataaa acaataatct
     6841 ttaagcaatc ctcaggaggg gacccagaaa ttgtaacgca cagttttaat tgtggagggg
     6901 aatttttcta ctgtaattca acacaactgt ttaatagtac ttggtttaat agtacttgga
     6961 gtactgaagg gtcaaataac actgaaggaa gtgacacaat cacactccca tgcagaataa
     7021 aacaatttat aaacatgtgg caggaagtag gaaaagcaat gtatgcccct cccatcagtg
     7081 gacaaattag atgttcatca aatattactg ggctgctatt aacaagagat ggtggtaata
     7141 acaacaatgg gtccgagatc ttcagacctg gaggaggcga tatgagggac aattggagaa
     7201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7321 ggttcttggg agcagcagga agcactatgg gctgcacgtc aatgacgctg acggtacagg
     7381 ccagacaatt attgtctgat atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa acagctccag gcaagaatcc
     7501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     7561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     7621 agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat tacacaagct
     7681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     7741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     7801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     7861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     7921 tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     7981 acagagacag atccattcga ttagtgaacg gatccttagc acttatctgg gacgatctgc
     8041 ggagcctgtg cctcttcagc taccaccgct tgagagactt actcttgatt gtaacgagga
     8101 ttgtggaact tctgggacgc agggggtggg aagccctcaa atattggtgg aatctcctac
     8161 agtattggag tcaggaacta aagaatagtg ctgttaactt gctcaatgcc acagccatag
     8221 cagtagctga ggggacagat agggttatag aagtattaca agcagcttat agagctattc
     8281 gccacatacc tagaagaata agacagggct tggaaaggat tttgctataa gatgggtggc
     8341 aagtggtcaa aaagtagtgt gattggatgg cctgctgtaa gggaaagaat gagacgagct
     8401 gagccagcag cagatggggt gggagcagta tctcgagacc tagaaaaaca tggagcaatc
     8461 acaagtagca atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag
     8521 gaagaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     8581 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8641 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     8701 tggcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     8761 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     8821 ttgttacacc ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg
     8881 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     8941 aagaactgct gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     9001 cgtggcctgg gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct
     9061 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9121 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9181 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9241 gaaaatctct agcacccagg aggtagaggt tgcagtgagc caagatcgcg ccaccattcg
     9301 gggatcagag tttttaagga taacttagtg tgtagggggc cagtgagttg gagatgaaag
     9361 cgtagggagt cgaaggtgtc cttttgcgcc gagtcagttc ctgggtgggg gccacaagat
     9421 cggatgagcc agtttatcaa tccgggggtg ccagctgatc catggagtgc agggtctgca
     9481 aaatatctca agcactgatt gatcttaggt tttacaatag tgatgttacc ccaggaacaa
     9541 tttggggaag gtcagaatct tgtagcctgt agctgcatga ctcctaaacc ataatttctt
     9601 ttttgttttt ttttttttat ttttgagaca gggtctcact ctgtcaccta ggctggagtg
     9661 cagtggtgca atcacagctc actgcagcct caacgtcgta agctcaagcg atcctcccac
     9721 ctcagcctgc ctggtagctg agactacaag cgacgcccca gttaattttt gtatttttgg
     9781 tagaggcagc gttttgccgt gtggccctgg ctggtctcga actcctgggc tcaagtgatc
     9841 cagcctcagc ctcccaaagt gctgggacaa ccgggcccag tcactgcacc tggccctaaa
     9901 ccataatttc taatcttttg gctaatttgt tagtcctaca aaggcagtct agtccccagc
     9961 aaaaaggggg tttgtttcgg gaaagggctg ttactgtctt tgtttcaaac tataaactaa
    10021 gttcctccta aacttagttc ggcctacacc caggaatgaa caaggagagc ttggaggtta
    10081 gaagcacgat ggaattggtt aggtcagatc tctttcactg tctgagttat aattttgcaa
    10141 tggtggttca aagactgccc gcttctgaca ccagtcgctg cattaatgaa tcggccaacg
    10201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    10861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    12181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    12241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    12301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    12361 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    12421 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc cagagtcctt
    12481 ggtgtggagg gagggaccag cgcagcttcc agccatccac ctgatgaaca gaacctaggg
    12541 aaagccccag ttctacttac accaggaaag gctggaaggg ctaatttggt cccaaaaaag
    12601 acaagagatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
    12661 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct tcaagttagt
    12721 accagttgaa ccagagcaag tagaagaggc caatgaagga gagaacaaca gcttgttaca
    12781 ccctatgagc cagcatggga tggaggaccc ggagggagaa gtattagtgt ggaagtttga
    12841 cagcctccta gcatttcgtc acatggcccg agagctgcat
//
