LOCUS       sequence-460800-       16030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses entire mouse reelin ORF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   D'Arcangelo G, Nakajima K, Miyata T, Ogawa M, Mikoshiba K, Curran T
  TITLE     Reelin is a secreted glycoprotein recognized by the CR-50 monoclonal
            antibody.
  JOURNAL   J Neurosci. 1997 Jan 1;17(1):23-31.
  PUBMED    8987733
REFERENCE   2  (bases 1 to 16030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1997-01-1"; volume: "17(1)"; pages: "23-31"
COMMENT     Sequence Label: pCrl
FEATURES             Location/Qualifiers
     source          1..16030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2545..2556
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4597..4608)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(11532..11548)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11532..11548)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11583..11601)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11584..11601)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11621..11638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11627..11851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11897..12325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11984..12003)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12194..12215
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12334..12354)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12339..12668
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12519..12654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12581..12600
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             12735..13529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12789..12808)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13399..13418
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    13703..13824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13740..13759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13794..13813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13873..13889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13873..13889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13886..13908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13897..13913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13921..13927,13928..13945,13946..13951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13966..13987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14104..14121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14275..14863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14355..14374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15034..15825,15826..15894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15657..15676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15895..15999)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagag gctcgccttc
      961 cccgcacgcg gctcctccgt gccggtgcct ccgaaagtgg atgagagagc gcgcggggcg
     1021 cgcggcggca cggagcgcgg cggcatggag cgcggctgct gggcgccgcg ggctctcgtc
     1081 ctggccgtgc tgctgctgct ggcgacgctg agggcgcgcg cggccaccgg ctactacccg
     1141 cgcttctcgc ctttcttttt cctgtgcacc caccacgggg agctggaagg ggatggggag
     1201 cagggcgagg tgctcatttc cctgcacatt gcgggcaacc ccacctacta cgtaccggga
     1261 caggaatacc atgttacaat ttcaacaagc accttctttg atggcttgct ggtgacggga
     1321 ctctatacct cgacaagcat ccagtcttct cagagcattg gaggctccag cgcctttgga
     1381 ttcgggatca tgtccgacca ccagtttggt aaccagttta tgtgcagtgt ggtggcctct
     1441 catgtgagtc acctgcctac aaccaacctc agctttgtct ggattgcccc accagctggc
     1501 acaggctgtg tgaatttcat ggctactgca acacataggg gccaggtgat tttcaaagac
     1561 gcactggccc agcagctgtg tgaacaagga gctcccacag aggccactgc ttactcgcac
     1621 cttgctgaaa tacacagtga cagtgtgatc ctacgagatg actttgactc ctaccagcaa
     1681 ctggaattga accccaacat atgggttgaa tgcagcaact gtgagatggg agagcagtgt
     1741 ggcaccatca tgcatggcaa tgctgtcacc ttctgtgagc cgtacggccc tcgagagctg
     1801 accaccacat gcctgaacac aacaacagca tctgtcctcc agttttccat tgggtcagga
     1861 tcatgtcgat ttagttactc tgaccccagc atcactgtgt catacgccaa gaacaatacc
     1921 gctgattgga ttcagctgga gaaaattaga gccccttcca atgtgagcac agtcatccac
     1981 atcctgtacc tccccgagga agccaaaggg gagagcgtgc agttccagtg gaaacaggac
     2041 agcctgcgag tgggtgaggt gtatgaggcc tgctgggccc tggataacat cctggtcatc
     2101 aattcagccc acagagaagt cgttctggag gacaacctcg acccggtcga cacgggcaac
     2161 tggctcttct tccctggagc aacggtcaag catagctgtc agtcagatgg gaactccatt
     2221 tatttccatg gaaatgaagg cagcgagttc aattttgcca ccacccggga tgtagatctt
     2281 tctacagagg atattcaaga gcagtggtca gaagaatttg agagccagcc cacaggatgg
     2341 gatatcttgg gagcagtagt tggtgcagac tgtggaaccg tagaatcagg actatcactg
     2401 gtgttcctca aagatggaga gaggaagctt tgcaccccct acatggatac aactggttat
     2461 ggcaacctga ggttctactt cgttatggga ggaatctgtg accctggagt ctctcatgaa
     2521 aacgatatca tcttatatgc aaagattgaa ggaagaaaag aacacattgc actggacact
     2581 cttacctatt cttcctataa ggttccgtct ttggtttctg tggtcatcaa ccctgaactt
     2641 cagacacctg ccaccaaatt ttgtctcagg caaaagagcc accaagggta taatcggaat
     2701 gtctgggctg tggacttctt ccatgtgctg cccgttctcc cttcaacaat gtctcacatg
     2761 atccagtttt ctattaattt gggatgcggc acacaccagc ctgggaacag cgtcagcttg
     2821 gagttttcta ctaaccatgg acggtcctgg tccctactcc acactgagtg cttgccggag
     2881 atctgtgcag gcccccacct cccccacagc actgtctact cctcagaaaa ctacagcggg
     2941 tggaaccgaa tcacgattcc tctccctaat gcagcactca cccgagacac caggattcgc
     3001 tggagacaaa caggcccaat cctgggaaat atgtgggcaa ttgataatgt ttatataggt
     3061 ccttcgtgtc tcaaattctg ttctggcaga ggacaatgca ctcggcatgg ctgcaagtgt
     3121 gacccaggat tttctggccc agcttgtgag atggcatctc agacattccc aatgtttatt
     3181 tcggaaagct ttggcagtgc cagactttcc tcttaccata acttttactc tatccgtggt
     3241 gctgaagtca gctttggttg tggtgtctta gccagtggta aggctctggt tttcaacaaa
     3301 gatgggaggc ggcagctaat cacgtccttt ctggacagct cgcagtccag gtttcttcag
     3361 tttacactga ggctggggag caagtctgtg ctgagcacgt gcagagcccc tgaccagccg
     3421 ggggagggag tcctgctgca ctattcatat gacaacggga taacatggaa actcctggag
     3481 cactattcct acgtcaacta ccacgagccc agaataatct ctgtagagct accggatgat
     3541 gcaagacagt ttggaatcca gttcagatgg tggcagcctt accattcttc ccaaggagaa
     3601 gacgtgtggg ccattgatga gattgtcatg acctcagtcc tgttcaacag catcagtctc
     3661 gactttacca atcttgtgga agtcactcaa tccctgggat tctaccttgg caatgttcaa
     3721 ccatactgtg gccatgactg gacgctttgt tttacgggag attctaaact tgcctcaagc
     3781 atgcgctatg tggaaacaca gtccatgcag atcggagcat cctatatgat tcagttcagc
     3841 ctagtgatgg gatgtggcca gaaatacact cctcacatgg acaaccaggt gaagctggag
     3901 tactcagcca accacggcct tacatggcac cttgtacaag aagaatgcct tcccagtatg
     3961 ccaagctgcc aggaatttac atctgccagc atttaccatg ccagcgagtt cacacagtgg
     4021 agaagagtca ctgttgttct tccccagaaa acatggtccg gtgccacccg cttccgttgg
     4081 agtcagagct attacacagc ccaggatgag tgggctttag acaacattta cattgggcag
     4141 cagtgcccca acatgtgcag tgggcatggc tcatgtgacc atggcgtgtg caggtgtgac
     4201 cagggatacc agggcactga atgccaccca gaagctgcac ttccttccac gattatgtca
     4261 gattttgaga acccgagcag ttgggaatca gactggcagg aagttattgg gggagaagtt
     4321 gtaaagcctg agcaaggctg tggagtcgtg tcttctggat cttctctgta cttcagcaag
     4381 gctgggaaga ggcagctggt gagctgggac ctggacacat cctgggtgga ctttgtccag
     4441 ttctacatcc agataggagg agagagtgct gcatgcaaca agcctgacag cagagaggag
     4501 ggcattctgc tccagtatag caacaacggg ggcatccagt ggcacctgct ggcagagatg
     4561 tacttctcag acttcagcaa acccagattt gtctacctgg agctcccagc tgctgcgaag
     4621 accccttgta ccaggttccg ctggtggcag cctgtgttct cgggggagga ctatgaccag
     4681 tgggccgttg atgatatcat cattctgtca gagaagcaga agcaggttat tccagttgtc
     4741 aacccaactt tgccccagaa cttctatgag aagccagctt tcgattaccc tatgaaccaa
     4801 atgagtgtgt ggctaatgtt ggccaatgaa ggcatggcta aaaacgacag cttctgtgcg
     4861 accacgccgt cagccatggt gtttggaaag tcagatggag accggtttgc agtaactcga
     4921 gatctgaccc tgaaacctgg atatgtgctg cagttcaagc taaacatagg ctgcaccagc
     4981 cagttcagca gcactgcccc ggttctcctg cagtattcac atgatgccgg catgtcgtgg
     5041 tttctggtga aggaaggatg cttcccagcg tcagcaggca aaggatgtga agggaactcc
     5101 agggaattga gtgagcctac tgtctattat actggggact tcgaagaatg gactagaatc
     5161 accattgcca ttccaaggtc ccttgcatcc agcaagacca gattccgatg gatccaagag
     5221 agcagctctc agaagaatgt gcccccgttt ggcttagatg gggtgtacat atctgagcct
     5281 tgtcccagtt actgcagtgg ccatggagac tgcatctcgg gggtgtgttt ttgtgacctg
     5341 gggtacacag ctgcacaagg aacctgtgtg tcaaacaccc ctaaccacag tgagatgttc
     5401 gacaggtttg aggggaagct aagcccactg tggtacaaaa tcaccggggg tcaggttggc
     5461 acgggctgtg gcaccctcaa tgacggcagg tccctctact ttaatggcct tgggaaaagg
     5521 gaagccagga cagtcccact ggacaccagg aatatcagac ttgttcagtt ttatatacaa
     5581 attggaagta aaacatcagg gattacgtgc atcaaaccac gggctagaaa tgaggggctt
     5641 gttgttcagt attccaatga taatgggata ctttggcatt tgctgagaga gttggatttc
     5701 atgtcattcc tggagccaca gatcatttcc attgacctgc cccgggaagc aaagacacct
     5761 gccacagctt tccggtggtg gcagccgcag catgggaagc attcggccca gtgggctttg
     5821 gatgatgtcc ttataggagt gaatgacagc tctcaaactg gatttcaaga taaatttgat
     5881 ggctccatag acttgcaagc caactggtat cgaatccagg gaggccaagt tgatatcgac
     5941 tgcctctcta tggacactgc ccttatattc actgaaaaca taggaaaacc tcgctatgct
     6001 gagacctggg acttccatgt gtcagcgtca agcttcttac agtttgaaat gaacatgggc
     6061 tgcagcaagc ctttcagtgg tgcccacggc atacagctcc agtactctct gaacaacggc
     6121 aaggactggc agcttgtcac cgaagagtgt gtccctccaa ccattggctg cgtgcactac
     6181 acagagagtt caacttacac atcagaaaga ttccagaact ggaggcgggt cacggtctac
     6241 ctgccactcg ccaccaattc tcccaggact cggttcagat ggattcagac caactatact
     6301 gttggagcag attcctgggc tattgataat gtcatcctgg cctcgggctg tccttggatg
     6361 tgctcaggac gagggatctg tgattcgggg cgctgtgtgt gtgaccgggg cttcggtgga
     6421 cccttctgtg ttcctgttgt tcctcttccc tccattctaa aagatgattt caatgggaac
     6481 ttacatcctg acctttggcc tgaagtgtac ggggcagaga ggggcaatct gaatggcgaa
     6541 accatcaaat ccggaacatg tctgatcttt aaaggggagg gactaagaat gcttatttcc
     6601 agagatctag attgtaccaa tactatgtat gtccagttct ctctccgatt tatagcgaaa
     6661 ggtaccccag agaggtctca ctccatcctt ctacagttct ctgtcagtgg aggagtcacc
     6721 tggcacctga tggatgaatt ctacttccct caaacgacca gcatactttt catcaatgtt
     6781 cccttaccat acggtgccca aaccaacgct acaagattca gactctggca accgtacaat
     6841 aatggtaaga aagaagaaat ctggatcatt gatgacttta ttattgatgg aaacaatttg
     6901 aacaaccccg tgctgctgct ggacacgttc gactttgggc ccagggaaga caattggttt
     6961 ttctatccgg gtggtaatat cggactttac tgcccgtatt cttcaaaggg agctcctgag
     7021 gaggattcgg ccatggtgtt tgtttcaaac gaagttggag aacactccat taccacacga
     7081 gacctaagtg tgaacgagaa caccatcatt caatttgaga tcaatgttgg ctgctccact
     7141 gatagttctt ctgctgatcc ggtcagactg gaattctcaa gggactttgg agccacctgg
     7201 cacctgctgc tgcctctctg ctaccacagc agcagcctcg tcagctcctt atgctccact
     7261 gagcatcacc cgagcagcac ctactacgcg gggaccaccc agggctggcg gcgggaggtc
     7321 gtgcacttcg gaaagctgca cctttgtgga tctgtgcgtt tccgttggta ccagggattt
     7381 tatcctgctg gctctcagcc ggtcacatgg gccattgaca atgtctacat tggtccccag
     7441 tgtgaagaga tgtgctatgg gcacgggagc tgcatcaatg gaaccaagtg tatatgtgac
     7501 ccgggctact ctgggccaac ctgtaaaata agcaccaaaa atcctgattt tctcaaagac
     7561 gactttgaag gtcaactgga atccgatcga ttcttactga tgagcggtgg gaagccgtct
     7621 cgtaagtgtg gcatcctttc cagtgggaac aacctcttct tcaatgagga cggcttgcgc
     7681 atgctagtaa cacgggacct ggatttatca catgcaaggt ttgtgcagtt cttcatgaga
     7741 ctgggatgtg gtaaaggtgt tccagacccc aggagccagc ccgtgcttct gcagtactcc
     7801 ctcaatggcg gcctctcctg gagtcttctt caagagttcc tcttcagcaa ctccagcaat
     7861 gtgggcaggt acattgccct ggaaatgccc ctgaaagccc gttctggttc gacacgcctc
     7921 cgctggtggc agccatctga aaatgggcac ttctatagcc cctgggtgat cgaccagatt
     7981 cttattggag gaaatatctc tggtaataca gtcttagaag atgatttctc aactctggac
     8041 agcagaaagt ggctgcttca cccaggaggc accaagatgc ctgtgtgtgg ctccacaggc
     8101 gatgccctgg tctttattga aaaggccagc acccgttacg tggtcacgac agacatcgct
     8161 gtgaatgagg actcattcct acagatagac tttgctgcct cctgctcagt cacagactcc
     8221 tgctatgcta ttgaactgga gtactcggtg gatctcggtc tgtcgtggca cccgctggtg
     8281 agggactgcc tgcctaccaa tgttgagtgt agtcgttacc acctgcagcg gatcctggtg
     8341 tcagatactt tcaacaagtg gaccagaatc actctgcccc tgccttccta caccaggtct
     8401 caagccactc gtttccgctg gcatcagcca gcgccttttg acaagcagca gacctgggca
     8461 atagataatg tctatattgg ggatggttgc ctagacatgt gcagtggcca cgggagatgc
     8521 gtccagggaa gctgtgtctg tgatgaacag tggggaggcc tgtactgtga tgagcctgag
     8581 acctcccttc ccacccagct caaagacaac ttcaaccgag ccccctccaa ccagaactgg
     8641 ctgactgtga gcggtgggaa gctgagtaca gtgtgtgggg ctgtggcttc cggcctggct
     8701 ctccatttca gtgggggctg cagccgattg ttagtcactg tggatctgaa cctcaccaat
     8761 gctgagttta tccagtttta ctttatgtat ggatgcctca ttacgccgag caaccgtaac
     8821 cagggagtcc tgctggagta ctctgtcaat ggaggcatca cctggaactt gctgatggag
     8881 attttctatg accagtacag caaacctgga tttgtgaata tccttctccc tcctgatgct
     8941 aaagagattg ccactcgctt ccgatggtgg cagccacgac atgatggcct tgaccagaat
     9001 gactgggcca ttgacaatgt cctcatctcg ggctctgcgg accagaggac agtcatgctg
     9061 gacaccttta gcagcgcccc agtaccacag catgagcgct cccccgcaga cgctggccct
     9121 gttggaagaa ttgcttttga aatgttctta gaagacaaaa cttcagtgaa tgagaattgg
     9181 ctcttccatg atgactgtac agtggaaaga ttctgtgact cgccagatgg tgtcatgctc
     9241 tgtggcagcc atgatggacg agaggtgtat gcagtgactc atgacctgac gcccactgag
     9301 aactggatca tgcagttcaa gatctctgtt ggatgcaaag tgcctgaaaa aattgcccag
     9361 aatcaaattc acgtgcagtt ttctactgac tttggcgtga gctggagtta tttagtccct
     9421 cagtgcttac ccgccgaccc aaagtgttct ggaagcgttt ctcaaccgtc tgtgttcttc
     9481 ccaactgaag ggtggaaaag gatcacctac ccgcttcctg aaagcttaac ggggaatcct
     9541 gtaagattta ggttctacca aaagtactca gatgtgcagt gggcaattga caatttctac
     9601 cttggccctg gatgtttgga caactgtgga ggccacggag actgcctaaa ggaacagtgt
     9661 atctgtgacc caggctactc agggccaaac tgctacttaa ctcacagcct gaagactttc
     9721 ctgaaggagc gctttgacag tgaggagatc aagcctgact tatggatgtc cttggaaggc
     9781 ggaagcactt gtacagagtg cggggtcctc gccgagaaca ctgcactcta ttttggggga
     9841 tccactgtga gacaagctat tactcaagac ttagatctca gaggtgcaaa attcctgcag
     9901 tactggggac gtatcggcag tgagaacaac atgacatctt gccatcggcc tgtctgccgg
     9961 aaggaaggcg tgctgctgga cttctctacg gatggaggaa tcacttggac cttgcttcac
    10021 gagatggatt tccagaaata catttctgtg aggcacgact acatcctcct gcctgagggg
    10081 gccctcacca acacaactcg acttcgctgg tggcagcctt ttgtcatcag caatgggctc
    10141 gtggtttccg gggtggagcg tgcgcagtgg gcactggaca acattctgat tggtggagca
    10201 gaaatcaatc caagccaact ggtggacact ttcgatgacg aaggctcctc ccatgaagaa
    10261 aactggagtt tttaccctaa tgcagtaagg acagcaggat tctgtggcaa cccatccttc
    10321 cacctctact ggccaaataa aaagaaggac aagacccaca atgcactctc ctcccgagag
    10381 ctcattatac agccaggata catgatgcaa tttaaaattg tggtgggttg tgaagccact
    10441 tcatgtggtg accttcattc cgtgatgctg gagtacacca aggatgcaag gtccgattcc
    10501 tggcagctcg tgcagaccca gtgcctacct tcctcttcca atagcattgg ctgctccccg
    10561 ttccagttcc atgaagccac catttataat gctgtcaaca gctcaagctg gaagaggatc
    10621 accatccagc tcccagacca cgtctcgtca agtgccacac agttccgctg gatccagaag
    10681 ggagaagaaa ccgagaagca aagctgggcc atcgaccacg tgtacatcgg agaggcttgt
    10741 cccaagctct gcagcgggca tggctactgc accacagggg ccgtctgcat ctgcgatgaa
    10801 agcttccaag gtgacgactg ctctgtcttc agtcacgagc ttcctagtta cattaaagat
    10861 aattttgaat cagcaagagt cactgaagcc aactgggaaa ccatccaggg tggagtgatc
    10921 ggaagtggct gtgggcagct ggcgccctat gcccatggag attcgctcta ctttaatggt
    10981 tgtcagataa ggcaagctgc caccaagcca ctggacctca ctcgagcaag caaaattatg
    11041 tttgtcttgc aaattgggag cccagcccag acagacagtt gcaacagcga cctcagcggc
    11101 ccccacaccg tggacaaagc agtactgctg cagtacagtg tcaacaatgg catcacctgg
    11161 cacgtcatcg ctcagcacca gccgaaggac ttcacacaag ctcagcgggt gtcttacaac
    11221 gtccccctgg aagctcggat gaaaggagtt ctactgcgct ggtggcagcc acgccacaat
    11281 ggaacaggtc atgatcaatg ggctttggac catgtggagg tcgtcctagt aagcactcgc
    11341 aaacaaaatt acatgatgaa tttttcacgg caacatgggc tcaggcactt ctacaacaga
    11401 agacgaaggt cgcttaggcg atacccatga agaatccaag tttatttccc tttccagcgt
    11461 acaatgtgtc ccttcctggt tttttgaaac acctctcact gcatctgata tcgaattcct
    11521 gcagcccggg ggatccacta gttctagagc ggccgctcga gcatgcatct agagggccct
    11581 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
    11641 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
    11701 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    11761 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    11821 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
    11881 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11941 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    12001 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    12061 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    12121 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    12181 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    12241 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    12301 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
    12361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    12421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    12481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    12541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    12601 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    12661 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
    12721 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    12781 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    12841 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    12901 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    12961 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    13021 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    13081 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    13141 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    13201 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    13261 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    13321 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    13381 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    13441 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    13501 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
    13561 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    13621 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    13681 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    13741 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    13801 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    13861 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    13921 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    13981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    14041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    14101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    14161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    14221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    14281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    14341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    14401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    14461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    14521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    14581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    14641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    14701 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    14761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    14821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    14881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    14941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    15001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    15061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    15121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    15181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    15241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    15301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    15361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    15421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    15481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    15541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    15601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    15661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    15721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    15781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    15841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    15901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    15961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    16021 acctgacgtc
//
