LOCUS       sequence-46081-e       10906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10906)
  TITLE     NLdDIS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pfNL43-dGPE-EGFP
FEATURES             Location/Qualifiers
     source          1..10906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2807..2924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      4394..4403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4400..4402,4403..4405,4406..5116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4400..4402,4403..4405,4406..5116)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4445..4466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4706..4725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5053..5074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5638..5871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6056..6100
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6394..6420
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     LTR             6955..7588
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8577..8594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8748..9336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8828..8847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9507..10298,10299..10367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10130..10149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10368..10472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10540..10558
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10596..10618)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10718..10737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gccggtacat ggagtgtatt atgacccatc aaaagactta atagcagaaa
     1561 tacagaagca ggggcaaggc caatggacat atcaaattta tcaagagcca tttaaaaatc
     1621 tgaaaacagg aaaatatgca agaatgaagg gtgcccacac taatgatgtg aaacaattaa
     1681 cagaggcagt acaaaaaata gccacagaaa gcatagtaat atggggaaag actcctaaat
     1741 ttaaattacc catacaaaag gaaacatggg aagcatggtg gacagagtat tggcaagcca
     1801 cctggattcc tgagtgggag tttgtcaata cccctccctt agtgaagtta tggtaccagt
     1861 tagagaaaga acccataata ggagcagaaa ctttctatgt agatggggca gccaataggg
     1921 aaactaaatt aggaaaagca ggatatgtaa ctgacagagg aagacaaaaa gttgtccccc
     1981 taacggacac aacaaatcag aagactgagt tacaagcaat tcatctagct ttgcaggatt
     2041 cgggattaga agtaaacata gtgacagact cacaatatgc attgggaatc attcaagcac
     2101 aaccagataa gagtgaatca gagttagtca gtcaaataat agagcagtta ataaaaaagg
     2161 aaaaagtcta cctggcatgg gtaccagcac acaaaggaat tggaggaaat gaacaagtag
     2221 atgggttggt cagtgctgga atcaggaaag tactattttt agatggaata gataaggccc
     2281 aagaagaaca tgagaaatat cacagtaatt ggagagcaat ggctagtgat tttaacctac
     2341 cacctgtagt agcaaaagaa atagtagcca gctgtgataa atgtcagcta aaaggggaag
     2401 ccatgcatgg acaagtagac tgtagcccag gaatatggca gctagattgt acacatttag
     2461 aaggaaaagt tatcttggta gcagttcatg tagccagtgg atatatagaa gcagaagtaa
     2521 ttccagcaga gacagggcaa gaaacagcat acttcctctt aaaattagca ggaagatggc
     2581 cagtaaaaac agtacataca gacaatggca gcaatttcac cagtactaca gttaaggccg
     2641 cctgttggtg ggcggggatc aagcaggaat ttggcattcc ctacaatccc caaagtcaag
     2701 gagtaataga atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg
     2761 ctgaacatct taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2821 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2881 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2941 acagcagaga tccagtttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag
     3001 tagtaataca agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatca
     3061 gggattatgg aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt
     3121 aacacatgga aaagattagt aaaacaccat atgtatattt caaggaaagc taaggactgg
     3181 ttttatagac atcactatga aagtactaat ccaaaaataa gttcagaagt acacatccca
     3241 ctaggggatg ctaaattagt aataacaaca tattggggtc tgcatacagg agaaagagac
     3301 tggcatttgg gtcagggagt ctccatagaa tggaggaaaa agagatatag cacacaagta
     3361 gaccctgacc tagcagacca actaattcat ctgcactatt ttgattgttt ttcagaatct
     3421 gctataagaa ataccatatt aggacgtata gttagtccta ggtgtgaata tcaagcagga
     3481 cataacaagg taggatctct acagtacttg gcactagcag cattaataaa accaaaacag
     3541 ataaagccac ctttgcctag tgttaggaaa ctgacagagg acagatggaa caagccccag
     3601 aagaccaagg gccacagagg gagccataca atgaatggac actagagctt ttagaggaac
     3661 ttaagagtga agctgttaga cattttccta ggatatggct ccataactta ggacaacata
     3721 tctatgaaac ttacggggat acttgggcag gagtggaagc cataataaga attctgcaac
     3781 aactgctgtt tatccatttc agaattgggt gtcgacatag cagaataggc gttactcgac
     3841 agaggagagc aagaaatgga gccagtagat cctagactag agccctggaa gcatccagga
     3901 agtcagccta aaactgcttg taccaattgc tattgtaaaa agtgttgctt tcattgccaa
     3961 gtttgtttca tgacaaaagc cttaggcatc tcctatggca ggaagaagcg gagacagcga
     4021 cgaagagctc atcagaacag tcagactcat caagcttctc tatcaaagca gtaagtagta
     4081 catgtaatgc aacctataat agtagcaata gtagcattag tagtagcaat aataatagca
     4141 atagttgtgt ggtccatagt aatcatagaa tataggaaaa tattaagaca aagaaaaata
     4201 gacaggttaa ttgatagact aatagaaaga gcagaagaca gtggcaatga gagtgaagga
     4261 gaagtatcag cacttgtgga gatgggggtg gaaatggggc accatgctcc ttgggatatt
     4321 gatgatctgt agtgctacag aaaaattgtg ggtcacagtc tattatgggg tacctaaggc
     4381 ctatccacct gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4441 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4501 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4561 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4621 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4681 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4741 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4801 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4861 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4921 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4981 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5041 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5101 ggacgagctg tacaaggacg agctgtaagc tagcaaatta agagaacaat ttggaaataa
     5161 taaaacaata atctttaagc aatcctcagg aggggaccca gaaattgtaa cgcacagttt
     5221 taattgtgga ggggaatttt tctactgtaa ttcaacacaa ctgtttaata gtacttggtt
     5281 taatagtact tggagtactg aagggtcaaa taacactgaa ggaagtgaca caatcacact
     5341 cccatgcaga ataaaacaat ttataaacat gtggcaggaa gtaggaaaag caatgtatgc
     5401 ccctcccatc agtggacaaa ttagatgttc atcaaatatt actgggctgc tattaacaag
     5461 agatggtggt aataacaaca atgggtccga gatcttcaga cctggaggag gcgatatgag
     5521 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     5581 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5641 agctttgttc cttgggttct tgggagcagc aggaagcact atgggctgca cgtcaatgac
     5701 gctgacggta caggccagac aattattgtc tgatatagtg cagcagcaga acaatttgct
     5761 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaaacagct
     5821 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     5881 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5941 taaatctctg gaacagattt ggaataacat gacctggatg gagtgggaca gagaaattaa
     6001 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     6061 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     6121 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     6181 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     6241 gtttcagacc cacctcccaa tcccgagggg acccgacagg cccgaaggaa tagaagaaga
     6301 aggtggagag agagacagag acagatccat tcgattagtg aacggatcct tagcacttat
     6361 ctgggacgat ctgcggagcc tgtgcctctt cagctaccac cgcttgagag acttactctt
     6421 gattgtaacg aggattgtgg aacttctggg acgcaggggg tgggaagccc tcaaatattg
     6481 gtggaatctc ctacagtatt ggagtcagga actaaagaat agtgctgtta acttgctcaa
     6541 tgccacagcc atagcagtag ctgaggggac agatagggtt atagaagtat tacaagcagc
     6601 ttatagagct attcgccaca tacctagaag aataagacag ggcttggaaa ggattttgct
     6661 ataagatggg tggcaagtgg tcaaaaagta gtgtgattgg atggcctgct gtaagggaaa
     6721 gaatgagacg agctgagcca gcagcagatg gggtgggagc agtatctcga gacctagaaa
     6781 aacatggagc aatcacaagt agcaatacag cagctaacaa tgctgcttgt gcctggctag
     6841 aagcacaaga ggaggaagag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6961 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     7021 gctacttccc tgattggcag aactacacac cagggccagg ggtcagatat ccactgacct
     7081 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7141 gagagaacac cagcttgtta caccctgtga gcctgcatgg aatggatgac cctgagagag
     7201 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7261 atccggagta cttcaagaac tgctgacatc gagcttgcta caagggactt tccgctgggg
     7321 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatgctgca
     7381 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7561 ttttagtcag tgtggaaaat ctctagcacc caggaggtag aggttgcagt gagccaagat
     7621 cgcgccacca ttcggggatc agagttttta aggataactt agtgtgtagg gggccagtga
     7681 gttggagatg aaagcgtagg gagtcgaagg tgtccttttg cgccgagtca gttcctgggt
     7741 gggggccaca agatcggatg agccagttta tcaatccggg ggtgccagct gatccatgga
     7801 gtgcagggtc tgcaaaatat ctcaagcact gattgatctt aggttttaca atagtgatgt
     7861 taccccagga acaatttggg gaaggtcaga atcttgtagc ctgtagctgc atgactccta
     7921 aaccataatt tcttttttgt tttttttttt ttatttttga gacagggtct cactctgtca
     7981 cctaggctgg agtgcagtgg tgcaatcaca gctcactgca gcctcaacgt cgtaagctca
     8041 agcgatcctc ccacctcagc ctgcctggta gctgagacta caagcgacgc cccagttaat
     8101 ttttgtattt ttggtagagg cagcgttttg ccgtgtggcc ctggctggtc tcgaactcct
     8161 gggctcaagt gatccagcct cagcctccca aagtgctggg acaaccgggc ccagtcactg
     8221 cacctggccc taaaccataa tttctaatct tttggctaat ttgttagtcc tacaaaggca
     8281 gtctagtccc cagcaaaaag ggggtttgtt tcgggaaagg gctgttactg tctttgtttc
     8341 aaactataaa ctaagttcct cctaaactta gttcggccta cacccaggaa tgaacaagga
     8401 gagcttggag gttagaagca cgatggaatt ggttaggtca gatctctttc actgtctgag
     8461 ttataatttt gcaatggtgg ttcaaagact gcccgcttct gacaccagtc gctgcattaa
     8521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9181 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10501 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10561 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    10621 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10681 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    10741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    10801 aggccagagt ccttggtgtg gagggaggga ccagcgcagc ttccagccat ccacctgatg
    10861 aacagaacct agggaaagcc ccagttctac ttacaccagg aaaggc
//
