LOCUS       sequence-460829-       10382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 
            3'UTR::SEC cloned in the destination vector pHW1133 to swap driver 
            via RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1134
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1632..1955
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(1973..2006)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2016..2045
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3862..4185)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6103..6127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6130..7155
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6130..7155
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7156..7180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7533..7566)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7581..7614)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7632..7648)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7633..7649)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7673..7692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7674..7692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7702..7719)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7702..7718)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7711..7733)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7860..8315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7947..7966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8157..8178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8341..8445
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8446..8514,8515..9306)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8664..8683)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9477..10065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9966..9985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10219..10236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10346..10367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10382,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatgg gaccaaagaa gaagcgtaag gcctccatga
      601 agctcttgtc ctccatggag caagcctgcg acatctgccg tctcaagaaa ctccgttgct
      661 ccaaggagaa gccaaagtgc gccaagtgcc tcaagaacaa ctccgagtgc cgttactccc
      721 caaaggccat gcgttcccca ctgactcgtg cccacctcac cgaggtcgag tcccgtctcg
      781 agaagctcga agacctcttc ctcctcatgt tcccacgtga gaacctggac tccatcctca
      841 acatggactc cctcgacgag gtcaaggtaa gtttaaacat atatatacta actaaccctg
      901 attatttaaa ttttcaggtc atgctcaagc aactctacat gcaagactac ggagacaccg
      961 acgctgccat ggactccctc accccaatgg agaacggaat gtctgaggga ccacgtaagc
     1021 acatgaagac cgccacctca tccccaaaag tcgacgagga cggatcccaa tcccagctct
     1081 ccgtctccaa gggttctgga tctggatctg gaggttccgg tcgtcaactt gagctcccac
     1141 caaccgccac cgccaccgcc agtatcatgc cacacgtcat ggagcaacca ctctccacct
     1201 ccatcaaccc agtcaacgac cgtttcaacg gaataccaaa cccaacccca tacaactccg
     1261 acgccgccct cgacgccatc acccaaacca acgactacgg atccgtcaac acccacggaa
     1321 tcctctccac ctacccacca ccagccaccc acctcaacga ggcctccgtc gccctcgcac
     1381 caggaggagc accaccacgt ccaccaccac catacgtcga ctccaccacc aaccacccac
     1441 cataccattc caacctcgtc ccaatggcca acttcggata ctccaccgtc gactacgacg
     1501 ccatggtcga cgacctcgcc tccatcgagt acaccgacgc cgtcgacgtc gacccacaat
     1561 tcatgaccaa cctcgggttc gtcccaggat gcaacttctc cgacatcaac acctatgagc
     1621 aataaacgta aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct
     1681 ttttgtcaaa aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact
     1741 ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct
     1801 gtctgatgta ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag
     1861 gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa
     1921 agaggaaact ttattgtgcc gccaagaaaa aagtctcaaa ggtaagttta aaataacttc
     1981 gtatagcata cattatacga agttattttc agggagagca aaagcttatc tccgaggagg
     2041 acctctaagg atgatcgacg ccaacgtcgt tgaattttca aattttaaat actgaatatt
     2101 tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa
     2161 ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat tcgtattccg
     2221 ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg
     2281 acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg
     2341 acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat
     2401 aaaaatatat agaggaggaa ggaaactagc tgccctcgat tgtctatctt ccattctcac
     2461 caagtttcaa caactttatt cagtcattct tctctggttc atttcccaac caatcttccc
     2521 gaataaagcc catctgtcct ttcggccaca ttcctcacct acatccgtac tccttatctc
     2581 ccgttcttat ccagtctttt atcgtctctg cgtctcgcac actgtctcgt cggtaatgta
     2641 aaaaaaaaac aaaaaaaact tgaaaacttg cggaaagatt tcctaataaa aggagacgaa
     2701 tccggtagga aatcgacaag tctctgcttc tcgaagacta ggtgagttac atttctttgg
     2761 ccgaattatt ttgaaatcct aaatttgata atttcagaga tgtctgtaaa acttgcgtgt
     2821 tatgtgacgg cttcggtcac tgtcgccact cttatggtat gcttcatgac catgtcaacc
     2881 atctactcgg aggttgatgg attcagagag aagcttgata ccgagatgaa tgtgttcaga
     2941 gtgagttaaa ttatattttg aattttaaat aatttttaat tttctagcaa tctaccaatg
     3001 gattgtggaa ggacatagtt gtcatcggaa gatctagcaa gcgtgtccgt tgtcaatatg
     3061 aagagaccaa cgctacccca actccacatg ctgatggatc cccatctgct ccaccaggtc
     3121 aaccaccagc agttccacca gtcttcaacc agccaaagac tccaaatgga gccaatggaa
     3181 atggaccaac ctgcaactgc aatgctgata acaagtgccc agctggacca tccggaccaa
     3241 agggagttcc aggagttcca ggactcgacg gagttccagg acttgacggt gttccaggag
     3301 ttggagctga tgatatcgct ccacaacgcg agtctgtcgg atgcttcact tgcccacaag
     3361 gaccagttgg accaccagga gctcttggaa gaccaggacc acgtggactt ccaggaccaa
     3421 gaggacaaaa tggaaaccca ggaagagatg gacaaccagg acatccagga gagcaaggat
     3481 catccggtca aatcggaaag atcggagagc caggaccacc aggagagaag ggacgcgacg
     3541 ccgagcatcc aatcggaaga ccaggaccaa agggaccaag aggagatcaa ggaccaacag
     3601 gaccagctgg acagaacggt cttcacggac caccaggaga gccaggaacc gttggaccag
     3661 aaggaccatc tggaaagcaa ggacgtcaag gaccagacgg aacccaggga gagactggac
     3721 cagacggaag accaggaaag gatgccgagt actgccagtg cccagacaag tctccaccat
     3781 cagaggctgt caacgccaac cgtggataca gaaatatcta aattgttggt gttttctaat
     3841 aaaatatttg agattcagtg agactttttt cttggcggca caataaagtt tcctcttttt
     3901 gttgggtcac tcaacgaaca caatacaaat atttgagcgc tcgcattttt ctcaaccttt
     3961 cgggtttaaa gcacttcaaa atggaattga agagagattg tgaaagtaca tcagacagaa
     4021 tcacattttg atagcattca cttcactcag atgcaaactt ttcagtgact ataaaaagtc
     4081 ataaaataat aacattaaaa aagcaaataa attagcgaga gaattttttg acaaaaagaa
     4141 agaagagtga tagagaagaa gggaatgctt gaaaggattt tgcatttatc ttaatcaccg
     4201 tcttcgagaa gacgaaccat ggcgccagtt tcagaatcca agtttctaat atagttcatc
     4261 acaatgttga cgttggtcca acctccggct tgcataatct ctgggatgga aactccagcg
     4321 cgagccatgt cacgggcagc tccgacacgg gcggaatgtc cagaccaagc caagtaacgt
     4381 tgtccggaat catccttggc tccgtagatg agacggtggg tagcttcgaa gattcctgaa
     4441 aatttatttt cagcttattc ttagctaata tgttgtttaa acttaccttc aagagctcga
     4501 gtcgagagtt gtgatgttgc actcggggca gcaactccgt tcttgcgaac acgacagaac
     4561 aggtagttgt ttggatcatc ggcgactccg gaaaccgaaa tccagcgctc aacgagcttg
     4621 gtaactccaa gggagagcgc tttttcgact ccggcagtcg agaccagcgt ttttgtacgt
     4681 ccgatgtgga tgagcattct tcctccgtct gtgcgactaa tatccttgac gcgaatgcgg
     4741 gcaatttcgg cgatacggag aagtgtgttg taagcaattc cgaggaaggc gaggtttcgg
     4801 atgtcctggc aacgatcgga attttccata agagaacgga cctgatcgaa gtcggttctg
     4861 aaaatttcgt caagtcttag gattgaatac attaatattt aaacttacct ttcgaaggcg
     4921 agggcttgct tggctctctc tccagcatca acattttcct tgcggatacg tctcattacg
     4981 agagatacgg cattagaatc tgatgggcgt ggaagtccag atcggcgatg gagcatattg
     5041 agctgtccga gatgttgttg gatcgtcttc accgccaatc cacgagcttg gaggtagagc
     5101 aagtagtcac gaacgtcttc tggctcggct gggaaccact tgcggttgtt aagcttgcac
     5161 caggcagccc aagaacggca cactgagaga agcatcttcc aggtgtgctc ggagaaggct
     5221 tgtctgtcac ggaacatatc catgaggttc ttgcgaactt catcacttgt cgcgtcaact
     5281 ggaagtgctg gaagattttg atgaacagtg agaagatttg acatattttc gaagtttttt
     5341 agatgcacta gaacaaagcg tgttggcttc ctctgagccc gctttcctta tatacccgca
     5401 ttctgcagcc ttacagaatg ttctagaagg tcctagatgc attcgtttga aaatactccc
     5461 ggtgggtgca aagagacgca gacggaaaat gtatctgggt ctctttattg tgtacactac
     5521 ttttccatgt accgaatgtg agtcgccctc cttttgcaac aagcagctcg aatgttctag
     5581 aaaaaggtgg aaaatagtat aaataccgtt gaaaataaat accgaacaac atttgctcta
     5641 attgtgaaat tagaaatctt caaactataa tcatgtcact ttaccactat ttccgtccag
     5701 ctcaacgttc cgtttttggt gatcattttt gctttcgtcg taaatctaca cacgcgtctc
     5761 ttccgtgcga gagtccaagc cagcagccaa attcgttgac tgagtattca acgtttatac
     5821 gttgtcggca acgagaaata ggaaaatgca tcgggaaatg ttcttttttc gattttttcc
     5881 aaggttttga caaattttac cacgaatttt gctatgtttt caattaaaaa atatgttatt
     5941 caactgtttc tatgaggaaa ataaggcttt gcatgtaatt ttcttattca gcataatttt
     6001 taattaattt gaattttctg tcctaacgtt tattttgttt tcttggttat gactgatctg
     6061 aaattaattt ttgaatttta aggtaatatg tcaggcggtg ccgcaagttt gtacaaaaaa
     6121 gcaggctcca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     6181 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     6241 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     6301 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     6361 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     6421 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     6481 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     6541 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     6601 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     6661 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     6721 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     6781 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     6841 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     6901 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     6961 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     7021 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     7081 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     7141 agggcaaagg aatagaccca gctttcttgt acaaagtggg tccaattact cttcaacatc
     7201 cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc
     7261 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     7321 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     7381 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta
     7441 cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     7501 tgttacgttt cagtttatga ccgcaatttt taataacttc gtatagcata cattatacga
     7561 agttattttc agggtcccgg gaagttccta tactatttga agaataggaa cttccccggg
     7621 atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag
     7681 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7741 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7801 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7861 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7921 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7981 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8041 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8101 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8161 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8221 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8281 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     8341 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8401 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8461 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8521 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8581 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8641 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8701 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8761 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8821 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8881 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8941 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9001 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9061 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9121 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9181 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9241 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9301 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9361 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9421 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9481 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9541 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9601 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9661 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9721 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9781 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9841 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10021 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10081 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10141 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10201 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggcccaata cgcaaaccgc
    10261 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10321 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10381 ct
//
