LOCUS       sequence-460839-       10795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 
            3'UTR::SEC cloned in the destination vector pHW1133 to swap driver 
            via RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1177
FEATURES             Location/Qualifiers
     source          1..10795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2386..2419)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2429..2458
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(4275..4598)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6516..6540
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6543..7568
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6543..7568
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7569..7593)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7946..7979)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7994..8027)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8045..8061)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8046..8062)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8086..8105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8087..8105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8115..8132)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8115..8131)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8124..8146)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8273..8728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8360..8379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8570..8591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8754..8858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8859..8927,8928..9719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9077..9096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9890..10478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10379..10398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10632..10649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10759..10780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10795,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatgt cacgtctaga taaatccaag gtcatcaact
      601 ccgccctcga gctcctcaat gaggtcggaa ttgagggact caccacccgt aagctcgccc
      661 aaaagctcgg agttgagcaa ccaaccctct actggcacgt caagaacaag cgtgcacttc
      721 tcgacgccct cgccatcgag atgctcgatc gtcaccacac ccacttctgc ccactcgagg
      781 gagaatcctg gcaagatttc ctccgtaaca acgccaagtc cttccgttgc gcccttctct
      841 cccaccgtga cggagccaag gtaagtttaa acatatatat actaactaac cctgattaat
      901 gaaattttca ggtccatctc ggaacccgtc caaccgagaa gcaatacgag actctcgaga
      961 accaacttgc cttcttgtgc caacaaggat tctccctcga aaacgccctc tacgccctct
     1021 ccgccgtcgg acatttcacc ctcggatgcg tcctcgaaga tcaagaacat caagttgcaa
     1081 aagaagaacg agaaactcca acaactgatt caatgccacc acttttgaga caagctatcg
     1141 aacttttcga tcaccaagga gcagaaccag ctttcctttt tggactggaa cttattatct
     1201 gtggacttga gaaacaactg aaatgtgaat cgggaggagg agcgggttct ggatctggat
     1261 ctggaggttc cggtcgtcaa cttgagctcc caccaaccgc caccgccacc gccagtatca
     1321 tgccacacgt catggagcaa ccactctcca cctccatcaa cccagtcaac gaccgtttca
     1381 acggaatacc aaacccaacc ccatacaact ccgacgccgc cctcgacgcc atcacccaaa
     1441 ccaacgacta cggatccgtc aacacccacg gaatcctctc cacctaccca ccaccagcca
     1501 cccacctcaa cgaggcctcc gtcgccctcg caccaggagg agcaccacca cgtccaccac
     1561 caccatacgt cgactccacc accaaccacc caccatacca ttccaacctc gtcccaatgg
     1621 ccaacttcgg atactccacc gtcgactacg acgccatggt cgacgacctc gcctccatcg
     1681 agtacaccga cgccgtcgac gtcgacccac aattcatgac caacctcggg ttcgtcccag
     1741 gatgcaactt ctccgacatc aacacctatg agcaataaaa gtaaattttt tgccccttcc
     1801 acccacaacg ctcaaaagaa gttcgtatta tcccgcaacc catccagacc atccttttac
     1861 atttttttta ttttctcttt tttttttctt caccctctcc gtgtacattg ctagtaatag
     1921 ttccaacttc tctagccgac tgcattatgc ttcccattct cacccaaatc ttcattggta
     1981 acttctcttg tatttgttca atactattcc ggtagtaatc taggcatgta taactaggtt
     2041 ctctgtgtat ctttggtttg aagttgaggt ttaatgcaat cacttttgtt gttgtaccct
     2101 attactgccg atgaccccca ttttctcatt acaaaattga cattttaaac cattttttct
     2161 ctgaatcatg ctcattattt gagccacatt cataataaag cttttaataa gacaaattga
     2221 tcaagtttat ctaatgtaaa aaatacaaat aaagaaaatt caacccggag gagagatgaa
     2281 gtgagtagct gttacggaac acagattatt tgccttgttt gtttccgaag atgtctccga
     2341 cgcggtccaa aattgagcca atactgagcg gaaggtaagt ttaaaataac ttcgtatagc
     2401 atacattata cgaagttatt ttcagggaga gcaaaagctt atctccgagg aggacctcta
     2461 aggatgatcg acgccaacgt cgttgaattt tcaaatttta aatactgaat atttgttttt
     2521 tttcctatta tttatttatt ctctttgtgt tttttttctt gctttctaaa aaattaattc
     2581 aatccaaatc taaacatttt tttttctctt tccgtctccc aattcgtatt ccgctcctct
     2641 catctgaaca caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg
     2701 gggaattggt ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca
     2761 atatgcaaca acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata
     2821 tatagaggag gaaggaaact agctgccctc gattgtctat cttccattct caccaagttt
     2881 caacaacttt attcagtcat tcttctctgg ttcatttccc aaccaatctt cccgaataaa
     2941 gcccatctgt cctttcggcc acattcctca cctacatccg tactccttat ctcccgttct
     3001 tatccagtct tttatcgtct ctgcgtctcg cacactgtct cgtcggtaat gtaaaaaaaa
     3061 aacaaaaaaa acttgaaaac ttgcggaaag atttcctaat aaaaggagac gaatccggta
     3121 ggaaatcgac aagtctctgc ttctcgaaga ctaggtgagt tacatttctt tggccgaatt
     3181 attttgaaat cctaaatttg ataatttcag agatgtctgt aaaacttgcg tgttatgtga
     3241 cggcttcggt cactgtcgcc actcttatgg tatgcttcat gaccatgtca accatctact
     3301 cggaggttga tggattcaga gagaagcttg ataccgagat gaatgtgttc agagtgagtt
     3361 aaattatatt ttgaatttta aataattttt aattttctag caatctacca atggattgtg
     3421 gaaggacata gttgtcatcg gaagatctag caagcgtgtc cgttgtcaat atgaagagac
     3481 caacgctacc ccaactccac atgctgatgg atccccatct gctccaccag gtcaaccacc
     3541 agcagttcca ccagtcttca accagccaaa gactccaaat ggagccaatg gaaatggacc
     3601 aacctgcaac tgcaatgctg ataacaagtg cccagctgga ccatccggac caaagggagt
     3661 tccaggagtt ccaggactcg acggagttcc aggacttgac ggtgttccag gagttggagc
     3721 tgatgatatc gctccacaac gcgagtctgt cggatgcttc acttgcccac aaggaccagt
     3781 tggaccacca ggagctcttg gaagaccagg accacgtgga cttccaggac caagaggaca
     3841 aaatggaaac ccaggaagag atggacaacc aggacatcca ggagagcaag gatcatccgg
     3901 tcaaatcgga aagatcggag agccaggacc accaggagag aagggacgcg acgccgagca
     3961 tccaatcgga agaccaggac caaagggacc aagaggagat caaggaccaa caggaccagc
     4021 tggacagaac ggtcttcacg gaccaccagg agagccagga accgttggac cagaaggacc
     4081 atctggaaag caaggacgtc aaggaccaga cggaacccag ggagagactg gaccagacgg
     4141 aagaccagga aaggatgccg agtactgcca gtgcccagac aagtctccac catcagaggc
     4201 tgtcaacgcc aaccgtggat acagaaatat ctaaattgtt ggtgttttct aataaaatat
     4261 ttgagattca gtgagacttt tttcttggcg gcacaataaa gtttcctctt tttgttgggt
     4321 cactcaacga acacaataca aatatttgag cgctcgcatt tttctcaacc tttcgggttt
     4381 aaagcacttc aaaatggaat tgaagagaga ttgtgaaagt acatcagaca gaatcacatt
     4441 ttgatagcat tcacttcact cagatgcaaa cttttcagtg actataaaaa gtcataaaat
     4501 aataacatta aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa gaaagaagag
     4561 tgatagagaa gaagggaatg cttgaaagga ttttgcattt atcttaatca ccgtcttcga
     4621 gaagacgaac catggcgcca gtttcagaat ccaagtttct aatatagttc atcacaatgt
     4681 tgacgttggt ccaacctccg gcttgcataa tctctgggat ggaaactcca gcgcgagcca
     4741 tgtcacgggc agctccgaca cgggcggaat gtccagacca agccaagtaa cgttgtccgg
     4801 aatcatcctt ggctccgtag atgagacggt gggtagcttc gaagattcct gaaaatttat
     4861 tttcagctta ttcttagcta atatgttgtt taaacttacc ttcaagagct cgagtcgaga
     4921 gttgtgatgt tgcactcggg gcagcaactc cgttcttgcg aacacgacag aacaggtagt
     4981 tgtttggatc atcggcgact ccggaaaccg aaatccagcg ctcaacgagc ttggtaactc
     5041 caagggagag cgctttttcg actccggcag tcgagaccag cgtttttgta cgtccgatgt
     5101 ggatgagcat tcttcctccg tctgtgcgac taatatcctt gacgcgaatg cgggcaattt
     5161 cggcgatacg gagaagtgtg ttgtaagcaa ttccgaggaa ggcgaggttt cggatgtcct
     5221 ggcaacgatc ggaattttcc ataagagaac ggacctgatc gaagtcggtt ctgaaaattt
     5281 cgtcaagtct taggattgaa tacattaata tttaaactta cctttcgaag gcgagggctt
     5341 gcttggctct ctctccagca tcaacatttt ccttgcggat acgtctcatt acgagagata
     5401 cggcattaga atctgatggg cgtggaagtc cagatcggcg atggagcata ttgagctgtc
     5461 cgagatgttg ttggatcgtc ttcaccgcca atccacgagc ttggaggtag agcaagtagt
     5521 cacgaacgtc ttctggctcg gctgggaacc acttgcggtt gttaagcttg caccaggcag
     5581 cccaagaacg gcacactgag agaagcatct tccaggtgtg ctcggagaag gcttgtctgt
     5641 cacggaacat atccatgagg ttcttgcgaa cttcatcact tgtcgcgtca actggaagtg
     5701 ctggaagatt ttgatgaaca gtgagaagat ttgacatatt ttcgaagttt tttagatgca
     5761 ctagaacaaa gcgtgttggc ttcctctgag cccgctttcc ttatataccc gcattctgca
     5821 gccttacaga atgttctaga aggtcctaga tgcattcgtt tgaaaatact cccggtgggt
     5881 gcaaagagac gcagacggaa aatgtatctg ggtctcttta ttgtgtacac tacttttcca
     5941 tgtaccgaat gtgagtcgcc ctccttttgc aacaagcagc tcgaatgttc tagaaaaagg
     6001 tggaaaatag tataaatacc gttgaaaata aataccgaac aacatttgct ctaattgtga
     6061 aattagaaat cttcaaacta taatcatgtc actttaccac tatttccgtc cagctcaacg
     6121 ttccgttttt ggtgatcatt tttgctttcg tcgtaaatct acacacgcgt ctcttccgtg
     6181 cgagagtcca agccagcagc caaattcgtt gactgagtat tcaacgttta tacgttgtcg
     6241 gcaacgagaa ataggaaaat gcatcgggaa atgttctttt ttcgattttt tccaaggttt
     6301 tgacaaattt taccacgaat tttgctatgt tttcaattaa aaaatatgtt attcaactgt
     6361 ttctatgagg aaaataaggc tttgcatgta attttcttat tcagcataat ttttaattaa
     6421 tttgaatttt ctgtcctaac gtttattttg ttttcttggt tatgactgat ctgaaattaa
     6481 tttttgaatt ttaaggtaat atgtcaggcg gtgccgcaag tttgtacaaa aaagcaggct
     6541 ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     6601 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     6661 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     6721 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     6781 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     6841 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     6901 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     6961 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     7021 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     7081 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     7141 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     7201 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     7261 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     7321 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     7381 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     7441 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     7501 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     7561 aggaatagac ccagctttct tgtacaaagt gggtccaatt actcttcaac atccctacat
     7621 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     7681 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     7741 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     7801 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     7861 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     7921 tttcagttta tgaccgcaat ttttaataac ttcgtatagc atacattata cgaagttatt
     7981 ttcagggtcc cgggaagttc ctatactatt tgaagaatag gaacttcccc gggatcaagc
     8041 ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg
     8101 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8161 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8221 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8641 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8701 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8821 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8881 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8941 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9001 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9061 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9121 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9181 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9241 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9301 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9361 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9421 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9481 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9541 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9601 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9661 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10621 cgaccgagcg cagcgagtca gtgagcgagg aagcggccca atacgcaaac cgcctctccc
    10681 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    10741 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggct
//
