LOCUS       sequence-460840-       12034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in 
            the destination vector pHW1133 to swap driver via RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 12034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1221
FEATURES             Location/Qualifiers
     source          1..12034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3625..3658)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3668..3697
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(5514..5837)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7755..7779
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7782..8807
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7782..8807
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8808..8832)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9185..9218)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(9233..9266)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9284..9300)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9285..9301)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9325..9344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9326..9344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9354..9371)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9354..9370)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9363..9385)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9512..9967
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9599..9618)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9809..9830
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9993..10097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10098..10166,10167..10958)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10316..10335)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11129..11717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11618..11637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11871..11888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11998..12019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12034,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatgc caccaaaacg caagacactc aatgccgctg
      601 ccgaagccaa tgcccacgct gatggccatg ctgatggcaa tgctgatggt catgtcgcta
      661 acactgcagc aagcagcaac aacgcccgtt ttgcggactt gaccaacatt gacacacccg
      721 gcctcggccc taccacgacg acgttacttg tcgagcccgc tcgttcgaaa cgccagagag
      781 tctcgagggc ctgtgatcag tgtcgagctg cacgtgaaaa gtgtgatgga atccagccgg
      841 cttgcttccc ctgtgtgtcg cagggccggt cgtgtaccta ccaggccagt cccaagaagc
      901 gaggagtcca gacgggctac atccgcactc tcgaactggc tctggcttgg atgttcgaga
      961 acgttgcccg cagcgaggac gccctccaca atcttttggt ccgtgatgct ggccagggca
     1021 gcgctctcct ggtcggcaaa gactcgcctg ctgcagaacg cctccatgca agatgggcga
     1081 cgagtcgagt caacaaaagc atcacccgtc ttctctcagg tcaggccgca caagatccat
     1141 ctgaagacgg ccaatccccg tccgaagaca taaatgtcca agatgccggg gcaaagacat
     1201 ccgacttccc tcatgcgcct cacttgactt tctcggcgcc taagtctagt acagctgaga
     1261 cacgcactct accaggcccg gtccgaccac ctatttcggc aaacaccctg gaaaacaacc
     1321 ttcagccaga tggaaccggg atagggaagc taccacccaa tcattggcgc ctgctggata
     1381 tctacttttc ctacacgcat tcttggctcc ctatcctcga gaagaaagac atgtaccaag
     1441 cattgtacca gtactctgaa caaggctcgt tgcttccctc tgcgaatgtc gagtctggcg
     1501 ttcatgccga gctttggagc gcgctcgccc tggcgtcctt ccaggctgct gctactgctg
     1561 catcgagtgc tacgggtcca gcttcagctg ctcatggcca tgacaatgcc atcaatcctt
     1621 cacctgcaga catatctgac acagcccgaa agctcatacc tttggaaagc gggccgttcc
     1681 aggttcagca ctgcagagcg ttgctgcttc tttgtctcgt aagccttggg cgggatgatt
     1741 gggagtctgc ttggttgctg gttggctttg cggtccgcgt cctacttgtt gttcgcaccc
     1801 agttgcctcc tgatgatgac cggccacgac caagaatgcg tgcgctgctc gtcgcgtgct
     1861 tcatcgtgga taccattgtg tctatgagac acaacgtgcc ggcccatctc aagccagacg
     1921 acattgcgga tctgccgtta cctgaagacg gtcaagatca atgggagccg tggacaccat
     1981 gtgagggctt aggcggtgaa cacaccatgc tgcaaatgtt gaggaacccg gcataccctt
     2041 taagcacatt caaccaccta tatggcgtga ccaagctggt tgctttggag cttctgccaa
     2101 gaatacgaac atcttcacag aacgctccct tggagttcag gtcgcggttg cagcaggtaa
     2161 tcggccacaa ttctcccttc agcgtctttg tcctttccca ggatacagca tcggcttttg
     2221 tgcctactgc ataccttacc cgtaccgttt atttatgggc agctgccttt tctgagcctc
     2281 tcaacgaaca ctactcgcat cttctgatcg aaactcttga tcagtatcag aagcggtttg
     2341 gtacatatgc aatcccacct ctgatccctt ctcttctaga ctcccttctt gctttaaaga
     2401 aacaatcaca ttcttcagag cggcatcgaa ggcacttgga agaacttttc cccgcctact
     2461 cctccatttg gcctcgggga ggccgacaca gcaatactgg cctccaaccc atacgacaac
     2521 ttgagcttcc gccgactgcg actgccactg caagtatcat gccccatgtc atggaacagc
     2581 ccctgtcaac gtcaataaat ccggtcaatg atcggtttaa tggaataccg aatcctaccc
     2641 cctacaatag cgatgcggcc ctagacgcaa ttactcaaac caatgactac ggatcagtca
     2701 atacccatgg cattctcagt acatatccac cacctgccac ccacttgaac gaggcctcgg
     2761 tggcccttgc gcctggaggt gctcctccca gaccaccccc gccatacgtt gatagtacaa
     2821 cgaaccatcc tccttaccat agtaacctcg tccccatggc caatttcgga tattcgactg
     2881 tagactatga tgcgatggtg gatgatctgg catcgatcga gtacacggat gcggttgacg
     2941 ttgatccgca gttcatgacg aacctcgggt ttgtcccagg gtgtaatttt agcgatatca
     3001 acacgtatga gcaatagaag taaatttttt gccccttcca cccacaacgc tcaaaagaag
     3061 ttcgtattat cccgcaaccc atccagacca tccttttaca ttttttttat tttctctttt
     3121 ttttttcttc accctctccg tgtacattgc tagtaatagt tccaacttct ctagccgact
     3181 gcattatgct tcccattctc acccaaatct tcattggtaa cttctcttgt atttgttcaa
     3241 tactattccg gtagtaatct aggcatgtat aactaggttc tctgtgtatc tttggtttga
     3301 agttgaggtt taatgcaatc acttttgttg ttgtacccta ttactgccga tgacccccat
     3361 tttctcatta caaaattgac attttaaacc attttttctc tgaatcatgc tcattatttg
     3421 agccacattc ataataaagc ttttaataag acaaattgat caagtttatc taatgtaaaa
     3481 aatacaaata aagaaaattc aacccggagg agagatgaag tgagtagctg ttacggaaca
     3541 cagattattt gccttgtttg tttccgaaga tgtctccgac gcggtccaaa attgagccaa
     3601 tactgagcgg aaggtaagtt taaaataact tcgtatagca tacattatac gaagttattt
     3661 tcagggagag caaaagctta tctccgagga ggacctctaa ggatgatcga cgccaacgtc
     3721 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     3781 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     3841 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     3901 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     3961 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     4021 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaacta
     4081 gctgccctcg attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt
     4141 cttctctggt tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca
     4201 cattcctcac ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc
     4261 tgcgtctcgc acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact
     4321 tgcggaaaga tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct
     4381 tctcgaagac taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga
     4441 taatttcaga gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca
     4501 ctcttatggt atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag
     4561 agaagcttga taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa
     4621 ataattttta attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg
     4681 aagatctagc aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca
     4741 tgctgatgga tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa
     4801 ccagccaaag actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga
     4861 taacaagtgc ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga
     4921 cggagttcca ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg
     4981 cgagtctgtc ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg
     5041 aagaccagga ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga
     5101 tggacaacca ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga
     5161 gccaggacca ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc
     5221 aaagggacca agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg
     5281 accaccagga gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca
     5341 aggaccagac ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga
     5401 gtactgccag tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata
     5461 cagaaatatc taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt
     5521 ttcttggcgg cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa
     5581 atatttgagc gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt
     5641 gaagagagat tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc
     5701 agatgcaaac ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat
     5761 aaattagcga gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc
     5821 ttgaaaggat tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag
     5881 tttcagaatc caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg
     5941 cttgcataat ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac
     6001 gggcggaatg tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga
     6061 tgagacggtg ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa
     6121 tatgttgttt aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg
     6181 cagcaactcc gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc
     6241 cggaaaccga aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga
     6301 ctccggcagt cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt
     6361 ctgtgcgact aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt
     6421 tgtaagcaat tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca
     6481 taagagaacg gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat
     6541 acattaatat ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat
     6601 caacattttc cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc
     6661 gtggaagtcc agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct
     6721 tcaccgccaa tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg
     6781 ctgggaacca cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga
     6841 gaagcatctt ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt
     6901 tcttgcgaac ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag
     6961 tgagaagatt tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct
     7021 tcctctgagc ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa
     7081 ggtcctagat gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa
     7141 atgtatctgg gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc
     7201 tccttttgca acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg
     7261 ttgaaaataa ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat
     7321 aatcatgtca ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt
     7381 ttgctttcgt cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc
     7441 aaattcgttg actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg
     7501 catcgggaaa tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt
     7561 ttgctatgtt ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct
     7621 ttgcatgtaa ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg
     7681 tttattttgt tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata
     7741 tgtcaggcgg tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca
     7801 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     7861 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     7921 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     7981 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     8041 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     8101 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     8161 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     8221 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     8281 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     8341 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     8401 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     8461 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     8521 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     8581 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     8641 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     8701 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     8761 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt
     8821 gtacaaagtg ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     8881 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     8941 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     9001 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     9061 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     9121 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     9181 tttaataact tcgtatagca tacattatac gaagttattt tcagggtccc gggaagttcc
     9241 tatactattt gaagaatagg aacttccccg ggatcaagct tatcgatacc gtcgacctcg
     9301 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9361 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9421 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9481 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9541 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9601 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9661 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9721 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9781 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9841 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9901 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9961 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10021 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10081 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10141 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10201 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10261 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10321 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10381 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10441 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10501 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10561 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10621 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10681 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10741 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10801 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10861 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10921 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10981 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11041 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11101 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11161 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11221 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11281 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11341 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11401 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11461 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11521 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11581 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11641 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11701 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11761 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11821 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11881 tgagcgagga agcggcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    12001 tgtgagttag ctcactcatt aggcacccca ggct
//
