LOCUS       sequence-460841-       10669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with QF2[QF(DBD)-linker-QFAD]-act-4 
            3'UTR::SEC cloned in the destination vector pHW1133 to swap driver 
            via RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1222
FEATURES             Location/Qualifiers
     source          1..10669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2260..2293)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2303..2332
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(4149..4472)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6390..6414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6417..7442
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6417..7442
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7443..7467)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7820..7853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7868..7901)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7919..7935)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7920..7936)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7960..7979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7961..7979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7989..8006)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7989..8005)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7998..8020)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8147..8602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8234..8253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8444..8465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8628..8732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8733..8801,8802..9593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8951..8970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9764..10352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10253..10272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10506..10523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10633..10654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10669,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatgc cgcctaaacg caagacactc aatgccgctg
      601 ccgaagccaa tgcccacgct gatggccatg ctgatggcaa tgctgatggt catgtcgcta
      661 acactgcagc aagcagcaac aacgcccgtt ttgcggactt gaccaacatt gacacacccg
      721 gcctcggccc taccacgacg acgttacttg tcgagcccgc tcgttcgaaa cgccagagag
      781 tctcgagggc ctgtgatcag tgtcgagctg cacgtgaaaa gtgtgatgga atccagccgg
      841 cttgcttccc ctgtgtgtcg cagggccggt cgtgtaccta ccaggccagt cccaagaagc
      901 gaggagtcca gacgggctac atccgcactc tcgaactggc tctggcttgg atgttcgaga
      961 acgttgcccg cagcgaggac gccctccaca atcttttggt ccgtgatgct ggccagggca
     1021 gcgctctcct ggtcggcaaa gactcgcctg ctgcagaacg cctgcatgca agatgggcga
     1081 cgagtcgagt caacaaaagc atcacccgtc ttctcgcggg ttctggatct ggatctggag
     1141 gttccggtcg tcaacttgag ctcccaccaa ccgccaccgc caccgccagt atcatgccac
     1201 acgtcatgga gcaaccactc tccacctcca tcaacccagt caacgaccgt ttcaacggaa
     1261 taccaaaccc aaccccatac aactccgacg ccgccctcga cgccatcacc caaaccaacg
     1321 actacggatc cgtcaacacc cacggaatcc tctccaccta cccaccacca gccacccacc
     1381 tcaacgaggc ctccgtcgcc ctcgcaccag gaggagcacc accacgtcca ccaccaccat
     1441 acgtcgactc caccaccaac cacccaccat accattccaa cctcgtccca atggccaact
     1501 tcggatactc caccgtcgac tacgacgcca tggtcgacga cctcgcctcc atcgagtaca
     1561 ccgacgccgt cgacgtcgac ccacaattca tgaccaacct cgggttcgtc ccaggatgca
     1621 acttctccga catcaacacc tatgagcaat aaaagtaaat tttttgcccc ttccacccac
     1681 aacgctcaaa agaagttcgt attatcccgc aacccatcca gaccatcctt ttacattttt
     1741 tttattttct cttttttttt tcttcaccct ctccgtgtac attgctagta atagttccaa
     1801 cttctctagc cgactgcatt atgcttccca ttctcaccca aatcttcatt ggtaacttct
     1861 cttgtatttg ttcaatacta ttccggtagt aatctaggca tgtataacta ggttctctgt
     1921 gtatctttgg tttgaagttg aggtttaatg caatcacttt tgttgttgta ccctattact
     1981 gccgatgacc cccattttct cattacaaaa ttgacatttt aaaccatttt ttctctgaat
     2041 catgctcatt atttgagcca cattcataat aaagctttta ataagacaaa ttgatcaagt
     2101 ttatctaatg taaaaaatac aaataaagaa aattcaaccc ggaggagaga tgaagtgagt
     2161 agctgttacg gaacacagat tatttgcctt gtttgtttcc gaagatgtct ccgacgcggt
     2221 ccaaaattga gccaatactg agcggaaggt aagtttaaaa taacttcgta tagcatacat
     2281 tatacgaagt tattttcagg gagagcaaaa gcttatctcc gaggaggacc tctaaggatg
     2341 atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt tttttttcct
     2401 attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca
     2461 aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg
     2521 aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat
     2581 tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc
     2641 aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga
     2701 ggaggaagga aactagctgc cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa
     2761 ctttattcag tcattcttct ctggttcatt tcccaaccaa tcttcccgaa taaagcccat
     2821 ctgtcctttc ggccacattc ctcacctaca tccgtactcc ttatctcccg ttcttatcca
     2881 gtcttttatc gtctctgcgt ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa
     2941 aaaaacttga aaacttgcgg aaagatttcc taataaaagg agacgaatcc ggtaggaaat
     3001 cgacaagtct ctgcttctcg aagactaggt gagttacatt tctttggccg aattattttg
     3061 aaatcctaaa tttgataatt tcagagatgt ctgtaaaact tgcgtgttat gtgacggctt
     3121 cggtcactgt cgccactctt atggtatgct tcatgaccat gtcaaccatc tactcggagg
     3181 ttgatggatt cagagagaag cttgataccg agatgaatgt gttcagagtg agttaaatta
     3241 tattttgaat tttaaataat ttttaatttt ctagcaatct accaatggat tgtggaagga
     3301 catagttgtc atcggaagat ctagcaagcg tgtccgttgt caatatgaag agaccaacgc
     3361 taccccaact ccacatgctg atggatcccc atctgctcca ccaggtcaac caccagcagt
     3421 tccaccagtc ttcaaccagc caaagactcc aaatggagcc aatggaaatg gaccaacctg
     3481 caactgcaat gctgataaca agtgcccagc tggaccatcc ggaccaaagg gagttccagg
     3541 agttccagga ctcgacggag ttccaggact tgacggtgtt ccaggagttg gagctgatga
     3601 tatcgctcca caacgcgagt ctgtcggatg cttcacttgc ccacaaggac cagttggacc
     3661 accaggagct cttggaagac caggaccacg tggacttcca ggaccaagag gacaaaatgg
     3721 aaacccagga agagatggac aaccaggaca tccaggagag caaggatcat ccggtcaaat
     3781 cggaaagatc ggagagccag gaccaccagg agagaaggga cgcgacgccg agcatccaat
     3841 cggaagacca ggaccaaagg gaccaagagg agatcaagga ccaacaggac cagctggaca
     3901 gaacggtctt cacggaccac caggagagcc aggaaccgtt ggaccagaag gaccatctgg
     3961 aaagcaagga cgtcaaggac cagacggaac ccagggagag actggaccag acggaagacc
     4021 aggaaaggat gccgagtact gccagtgccc agacaagtct ccaccatcag aggctgtcaa
     4081 cgccaaccgt ggatacagaa atatctaaat tgttggtgtt ttctaataaa atatttgaga
     4141 ttcagtgaga cttttttctt ggcggcacaa taaagtttcc tctttttgtt gggtcactca
     4201 acgaacacaa tacaaatatt tgagcgctcg catttttctc aacctttcgg gtttaaagca
     4261 cttcaaaatg gaattgaaga gagattgtga aagtacatca gacagaatca cattttgata
     4321 gcattcactt cactcagatg caaacttttc agtgactata aaaagtcata aaataataac
     4381 attaaaaaag caaataaatt agcgagagaa ttttttgaca aaaagaaaga agagtgatag
     4441 agaagaaggg aatgcttgaa aggattttgc atttatctta atcaccgtct tcgagaagac
     4501 gaaccatggc gccagtttca gaatccaagt ttctaatata gttcatcaca atgttgacgt
     4561 tggtccaacc tccggcttgc ataatctctg ggatggaaac tccagcgcga gccatgtcac
     4621 gggcagctcc gacacgggcg gaatgtccag accaagccaa gtaacgttgt ccggaatcat
     4681 ccttggctcc gtagatgaga cggtgggtag cttcgaagat tcctgaaaat ttattttcag
     4741 cttattctta gctaatatgt tgtttaaact taccttcaag agctcgagtc gagagttgtg
     4801 atgttgcact cggggcagca actccgttct tgcgaacacg acagaacagg tagttgtttg
     4861 gatcatcggc gactccggaa accgaaatcc agcgctcaac gagcttggta actccaaggg
     4921 agagcgcttt ttcgactccg gcagtcgaga ccagcgtttt tgtacgtccg atgtggatga
     4981 gcattcttcc tccgtctgtg cgactaatat ccttgacgcg aatgcgggca atttcggcga
     5041 tacggagaag tgtgttgtaa gcaattccga ggaaggcgag gtttcggatg tcctggcaac
     5101 gatcggaatt ttccataaga gaacggacct gatcgaagtc ggttctgaaa atttcgtcaa
     5161 gtcttaggat tgaatacatt aatatttaaa cttacctttc gaaggcgagg gcttgcttgg
     5221 ctctctctcc agcatcaaca ttttccttgc ggatacgtct cattacgaga gatacggcat
     5281 tagaatctga tgggcgtgga agtccagatc ggcgatggag catattgagc tgtccgagat
     5341 gttgttggat cgtcttcacc gccaatccac gagcttggag gtagagcaag tagtcacgaa
     5401 cgtcttctgg ctcggctggg aaccacttgc ggttgttaag cttgcaccag gcagcccaag
     5461 aacggcacac tgagagaagc atcttccagg tgtgctcgga gaaggcttgt ctgtcacgga
     5521 acatatccat gaggttcttg cgaacttcat cacttgtcgc gtcaactgga agtgctggaa
     5581 gattttgatg aacagtgaga agatttgaca tattttcgaa gttttttaga tgcactagaa
     5641 caaagcgtgt tggcttcctc tgagcccgct ttccttatat acccgcattc tgcagcctta
     5701 cagaatgttc tagaaggtcc tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag
     5761 agacgcagac ggaaaatgta tctgggtctc tttattgtgt acactacttt tccatgtacc
     5821 gaatgtgagt cgccctcctt ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa
     5881 atagtataaa taccgttgaa aataaatacc gaacaacatt tgctctaatt gtgaaattag
     5941 aaatcttcaa actataatca tgtcacttta ccactatttc cgtccagctc aacgttccgt
     6001 ttttggtgat catttttgct ttcgtcgtaa atctacacac gcgtctcttc cgtgcgagag
     6061 tccaagccag cagccaaatt cgttgactga gtattcaacg tttatacgtt gtcggcaacg
     6121 agaaatagga aaatgcatcg ggaaatgttc ttttttcgat tttttccaag gttttgacaa
     6181 attttaccac gaattttgct atgttttcaa ttaaaaaata tgttattcaa ctgtttctat
     6241 gaggaaaata aggctttgca tgtaattttc ttattcagca taatttttaa ttaatttgaa
     6301 ttttctgtcc taacgtttat tttgttttct tggttatgac tgatctgaaa ttaatttttg
     6361 aattttaagg taatatgtca ggcggtgccg caagtttgta caaaaaagca ggctccatga
     6421 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     6481 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     6541 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     6601 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     6661 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     6721 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     6781 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     6841 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     6901 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     6961 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     7021 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     7081 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     7141 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     7201 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     7261 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     7321 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     7381 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     7441 agacccagct ttcttgtaca aagtgggtcc aattactctt caacatccct acatgctctt
     7501 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt
     7561 gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa
     7621 ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct
     7681 atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt
     7741 tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag
     7801 tttatgaccg caatttttaa taacttcgta tagcatacat tatacgaagt tattttcagg
     7861 gtcccgggaa gttcctatac tatttgaaga ataggaactt ccccgggatc aagcttatcg
     7921 ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac
     7981 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     8041 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     8101 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     8161 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8221 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8281 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8341 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8401 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8461 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8521 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8581 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8641 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8701 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8761 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8821 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8881 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8941 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9001 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9061 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9121 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9181 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9241 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9301 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9361 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9421 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9481 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9541 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9601 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9661 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9721 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9781 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9841 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9901 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9961 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10021 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10081 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10141 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10261 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10321 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10381 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10441 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10501 agcgcagcga gtcagtgagc gaggaagcgg cccaatacgc aaaccgcctc tccccgcgcg
    10561 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10621 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggct
//
