LOCUS       sequence-460844-       10744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' 
            UTR::SEC cloned in the destination vector pHW1133 to swap driver via
            RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1643
FEATURES             Location/Qualifiers
     source          1..10744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2335..2368)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2378..2407
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(4224..4547)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6465..6489
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6492..7517
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6492..7517
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7518..7542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7895..7928)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7943..7976)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7994..8010)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7995..8011)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8035..8054)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8036..8054)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8064..8081)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8064..8080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8073..8095)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8222..8677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8309..8328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8519..8540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8703..8807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8808..8876,8877..9668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9026..9045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9839..10427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10328..10347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10581..10598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10708..10729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10744,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatgt cacgtctaga taaatccaag gtcatcaact
      601 ctgctctgga actgctgaat ggagtgggaa ttgaaggact tacaacccga aagcttgcac
      661 aaaaattggg agttgaacaa ccaacacttt actggcatgt taagaataag cgagcacttt
      721 tggatgctct cccaattgaa atgttggata gacatcatac tcattcatgt ccacttgaag
      781 gagaatcttg gcaagatttc ttgagaaaca acgcaaagtc ataccgatgc gctcttttgt
      841 ctcatagaga tggagcaaaa gttcatcttg gaacacgacc aactgaaaag caatacgaaa
      901 cattggaaaa ccaacttgct ttcttgtgtc aacaaggatt ttcattggaa aatgctcttt
      961 acgcattgtc tgctgttgga cattttacac ttggatgcgt tttggaagaa caagaacatc
     1021 aagttgcaaa agaagaacga gaaactccaa caactgattc aatgccacca cttttgaagc
     1081 aagctatcga acttttcgat agacaaggag cagaaccagc tttccttttt ggactggaac
     1141 ttattatctg tggacttgag aaacaactga aatgtgaatc gggaggagga gcgggttctg
     1201 gatctggatc tggaggttcc ggtcgtcaac ttgagctccc accaaccgcc accgccaccg
     1261 ccagtatcat gccacacgtc atggagcaac cactctccac ctccatcaac ccagtcaacg
     1321 accgtttcaa cggaatacca aacccaaccc catacaactc cgacgccgcc ctcgacgcca
     1381 tcacccaaac caacgactac ggatccgtca acacccacgg aatcctctcc acctacccac
     1441 caccagccac ccacctcaac gaggcctccg tcgccctcgc accaggagga gcaccaccac
     1501 gtccaccacc accatacgtc gactccacca ccaaccaccc accataccat tccaacctcg
     1561 tcccaatggc caacttcgga tactccaccg tcgactacga cgccatggtc gacgacctcg
     1621 cctccatcga gtacaccgac gccgtcgacg tcgacccaca attcatgacc aacctcgggt
     1681 tcgtcccagg atgcaacttc tccgacatca acacctatga gcaataaaag taaatttttt
     1741 gccccttcca cccacaacgc tcaaaagaag ttcgtattat cccgcaaccc atccagacca
     1801 tccttttaca ttttttttat tttctctttt ttttttcttc accctctccg tgtacattgc
     1861 tagtaatagt tccaacttct ctagccgact gcattatgct tcccattctc acccaaatct
     1921 tcattggtaa cttctcttgt atttgttcaa tactattccg gtagtaatct aggcatgtat
     1981 aactaggttc tctgtgtatc tttggtttga agttgaggtt taatgcaatc acttttgttg
     2041 ttgtacccta ttactgccga tgacccccat tttctcatta caaaattgac attttaaacc
     2101 attttttctc tgaatcatgc tcattatttg agccacattc ataataaagc ttttaataag
     2161 acaaattgat caagtttatc taatgtaaaa aatacaaata aagaaaattc aacccggagg
     2221 agagatgaag tgagtagctg ttacggaaca cagattattt gccttgtttg tttccgaaga
     2281 tgtctccgac gcggtccaaa attgagccaa tactgagcgg aaggtaagtt taaaataact
     2341 tcgtatagca tacattatac gaagttattt tcagggagag caaaagctta tctccgagga
     2401 ggacctctaa ggatgatcga cgccaacgtc gttgaatttt caaattttaa atactgaata
     2461 tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg ctttctaaaa
     2521 aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca attcgtattc
     2581 cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta
     2641 ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa
     2701 ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac
     2761 ataaaaatat atagaggagg aaggaaacta gctgccctcg attgtctatc ttccattctc
     2821 accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca accaatcttc
     2881 ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt actccttatc
     2941 tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc gtcggtaatg
     3001 taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata aaaggagacg
     3061 aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt acatttcttt
     3121 ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta aaacttgcgt
     3181 gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg accatgtcaa
     3241 ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg aatgtgttca
     3301 gagtgagtta aattatattt tgaattttaa ataattttta attttctagc aatctaccaa
     3361 tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc gttgtcaata
     3421 tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg ctccaccagg
     3481 tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg gagccaatgg
     3541 aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac catccggacc
     3601 aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg gtgttccagg
     3661 agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca cttgcccaca
     3721 aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac ttccaggacc
     3781 aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag gagagcaagg
     3841 atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga agggacgcga
     3901 cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc aaggaccaac
     3961 aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa ccgttggacc
     4021 agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg gagagactgg
     4081 accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca agtctccacc
     4141 atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg gtgttttcta
     4201 ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag tttcctcttt
     4261 ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt ttctcaacct
     4321 ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta catcagacag
     4381 aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga ctataaaaag
     4441 tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt tgacaaaaag
     4501 aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta tcttaatcac
     4561 cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta atatagttca
     4621 tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg gaaactccag
     4681 cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa gccaagtaac
     4741 gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg aagattcctg
     4801 aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct tcaagagctc
     4861 gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga acacgacaga
     4921 acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc tcaacgagct
     4981 tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc gtttttgtac
     5041 gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg acgcgaatgc
     5101 gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag gcgaggtttc
     5161 ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg aagtcggttc
     5221 tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac ctttcgaagg
     5281 cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata cgtctcatta
     5341 cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga tggagcatat
     5401 tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct tggaggtaga
     5461 gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg ttaagcttgc
     5521 accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc tcggagaagg
     5581 cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt gtcgcgtcaa
     5641 ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt tcgaagtttt
     5701 ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct tatatacccg
     5761 cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt gaaaatactc
     5821 ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat tgtgtacact
     5881 acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct cgaatgttct
     5941 agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca acatttgctc
     6001 taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact atttccgtcc
     6061 agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta cacacgcgtc
     6121 tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt caacgtttat
     6181 acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt tcgatttttt
     6241 ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa aaatatgtta
     6301 ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt cagcataatt
     6361 tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt atgactgatc
     6421 tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt ttgtacaaaa
     6481 aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     6541 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     6601 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     6661 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     6721 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     6781 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     6841 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     6901 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     6961 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     7021 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     7081 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     7141 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     7201 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     7261 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     7321 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     7381 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     7441 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     7501 cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta ctcttcaaca
     7561 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     7621 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     7681 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     7741 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     7801 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     7861 tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtatagca tacattatac
     7921 gaagttattt tcagggtccc gggaagttcc tatactattt gaagaatagg aacttccccg
     7981 ggatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccaa ttcgccctat
     8041 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8101 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8161 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8221 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8281 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8401 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8461 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8521 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8581 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8641 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8761 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8821 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8881 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8941 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9001 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9061 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9121 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9181 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9241 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9301 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9361 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9421 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9481 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9541 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9601 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9661 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9721 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9781 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9841 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9901 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9961 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10021 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10081 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10141 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10201 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10261 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10321 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10381 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10441 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10501 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10561 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggcccaa tacgcaaacc
    10621 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10681 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10741 ggct
//
