LOCUS       sequence-460886-        1668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of glutamate sensor with with improved 
            sensitivity and fast deactivation kinetics (4f = fast).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Aggarwal A, Negrean A, Chen Y, Iyer R, Reep D, Liu A, Palutla A, Xie
            ME, MacLennan BJ, Hagihara KM, Kinsey LW, Sun JL, Yao P, Zheng J, 
            Tsang A, Tsegaye G, Zhang Y, Patel RH, Arthur BJ, Hiblot J, Leippe 
            P, Tarnawski M, Marvin JS, Vevea JD, Turaga SC, Tebo AG, Carandini 
            M, Rossi LF, Kleinfeld D, Konnerth A, Svoboda K, Turner GC, Hasseman
            J, Podgorski K
  TITLE     Glutamate indicators with increased sensitivity and tailored 
            deactivation rates
  JOURNAL   bioRxiv 2025.03.20.643984
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.20.643984"
COMMENT     Sequence Label: pRSET-iGluSnFR4f
FEATURES             Location/Qualifiers
     source          1..1668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     1068..1089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1192..1213)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccgc cgcaggcagc
      121 acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga ctcttcagtg
      181 cctttctctt atcaagacag tcagcacaaa gtggtgggtt actcgcagga atactccaac
      241 gccattgttg aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt
      301 ccgattactt cacaaaaccg tattccacta ctgcaaaacg gcacttacga ttttgaatgt
      361 ggttctacca ccaacaacgt cgaacgccaa aaattcgcgg ctttctctga cactattttc
      421 gtggtcggta cgcgcctgtt gaccaaaaag ggtggcgata tcaaagattt tgccaacctg
      481 aaagacaaag ccgtagtcgt cacttccggc actacctctg aagttttgct caacaaactg
      541 aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg tgactctttc
      601 cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg atgacgtact gctggccggt
      661 gaacgtgcga aagcgaagaa accagacgac tgggaaatcg tcggcaagcc gcagtctcag
      721 gaggcctacg gttgtatgtt gcgtaaagat gatccgcagt tcaaaaagct gatggatgac
      781 accatcgctc aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa gtggttcaaa
      841 aatccaattc tggtgaacca cacggtctat atcaccgccg acaagcagaa gaacggcatc
      901 aaggcgaact tcttcatcca ccacaacgtg gaggacggca gcgtgcagct cgccgaccac
      961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1021 agctatcagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1081 gagttcgtga ccgccaccgg gaactctctc ggcatggacg agctgtacaa gggcggtacc
     1141 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1201 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     1261 accaacggca agctaaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1321 cccaccctcg tgaccaccct gagctacggc gtgcagtgct tcgcccgcta ccccgaccac
     1381 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1441 atcagcttca aggacgacgg aacctacaag acccgcgccg aggtgaagtt agagggcgac
     1501 accctggtga accgcatcga gctaaagggc atcgacttca aggaggacgg caacatcctg
     1561 gggcacaagc tggagtacaa ctttaacaac cccctggtca tgaatttcga actgtcagac
     1621 gaaatgaaag cactgttcaa agaaccgaat gacaaggcac tgaaataa
//
