LOCUS       sequence-46096-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant
            C1V1 (E122T/E162T) for fast and potent optical excitation at 
            red-shifted wavelengths..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(118..151)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aggttgatta tcgataagct tgatatcgaa ttcataactt cgtatagcat acattatacg
       61 aagttattct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaaata
      121 acttcgtata ggatacttta tacgaagtta tgctagccac catgtcgcgg aggccatggc
      181 ttcttgccct agcgctggca gtggcgctgg cggccggcag cgcaggagcc tcgactggca
      241 gtgacgcgac ggtgccggtc gcgactcagg atggccccga ctacgttttc caccgtgccc
      301 acgagcgcat gctcttccaa acctcataca ctcttgagaa caatggttct gttatttgca
      361 tcccgaacaa cggccagtgc ttctgcttgg cttggcttaa atccaacgga acaaatgccg
      421 agaagttggc tgccaacatt ctgcagtgga ttacttttgc gctttcagcg ctctgcctga
      481 tgttctacgg ctaccagacc tggaagtcta cttgcggctg ggagaccatt tacgtggcca
      541 cgatcgagat gatcaagttc atcatcgagt atttccatga gtttgacgaa cctgcggtga
      601 tctactcatc caacggcaac aagaccgtgt ggcttcgtta cgcgacctgg ctgctcacct
      661 gcccggtcct tctgatccat ctgagcaacc tcacaggcct gaaggacgat tatagcaaaa
      721 ggactatggg cctgttggtt tctgatgtgg gatgcatcgt gtggggcgca accagcgcca
      781 tgtgtacggg gtggacgaag atcctgttct tcctcatctc attgagctat ggtatgtata
      841 cctattttca tgctgctaaa gtttatatcg aa
//
