LOCUS       sequence-46099-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant
            C1V1 (E122T/E162T) for fast and potent optical excitation at 
            red-shifted wavelengths..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    236..269
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    320..353
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          393..596
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(416..436)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
       61 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      121 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      181 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatagggc gcgccataac
      241 ttcgtataat gtatgctata cgaagttatt aagaggtttc atattgctaa tagcagctac
      301 aatccagcta ccattctgca taacttcgta taaagtatcc tatacgaagt tattccggag
      361 tcgactctag aggatccggt acctcacgac acctgaaatg gaagaaaaaa actttgaacc
      421 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
      481 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
      541 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
      601 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
      661 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
      721 gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
      781 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggc
//
