LOCUS       sequence-46100-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant
            C1V1 (E122T/E162T) for fast and potent optical excitation at 
            red-shifted wavelengths..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..72)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(123..156)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat agcatacatt
       61 atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct gggttaaggc
      121 aaataacttc gtataggata ctttatacga agttatgcta gccaccatgt cgcggaggcc
      181 atggcttctt gccctagcgc tggcagtggc gctggcggcc ggcagcgcag gagcctcgac
      241 tggcagtgac gcgacggtgc cggtcgcgac tcaggatggc cccgactacg ttttccaccg
      301 tgcccacgag cgcatgctct tccaaacctc atacactctt gagaacaatg gttctgttat
      361 ttgcatcccg aacaacggcc agtgcttctg cttggcttgg cttaaatcca acggaacaaa
      421 tgccgagaag ttggctgcca acattctgca gtggattact tttgcgcttt cagcgctctg
      481 cctgatgttc tacggctacc agacctggaa gtctacttgc ggctgggaga ccatttacgt
      541 ggccacgatc gagatgatca agttcatcat cgagtatttc catgagtttg acgaacctgc
      601 ggtgatctac tcatccaacg gcaacaagac cgtgtggctt cgttacgcga cctggctgct
      661 cacctgcccg gtccttctga tccatctgag caacctcaca ggcctgaagg acgattatag
      721 caaaaggact atgggcctgt tggtttctga tgtgggatgc atcgtgtggg gcgcaaccag
      781 cgccatgtgt acggggtgga cgaagatcct gttcttcctc atctca
//
