LOCUS       sequence-46103-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, stabilized step 
            function opsin (SSFO) for optogenetic activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag cgccaccatg
      181 gactatggcg gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg
      241 gtggtgaacg ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa
      301 tctcgcggca cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga
      361 ttcagcattt tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg
      421 gaggagatct atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag
      481 tttaagaatc cctctatgct ctaccttgcc acaggacacc gggtgcagtg gctgcgctat
      541 gcagagtggc tgctcacttc tcctgtcatc cttatccacc tgagcaacct caccggcctg
      601 agcaacgact acagcaggag aaccatggga ctccttgtct cagccatcgg gactatcgtg
      661 tggggggcta ccagcgccat ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga
      721 ttgtgctatg gcgcgaacac attttttcac gccgccaaag catatatcga gggttatcat
      781 actgtgccaa agggtcggtg ccgccaggtc gtgaccggca tggcatggct gtttttcgtg
      841 agctggggta tgttcccaat tctcttcatt ttggggcccg aaggttttgg
//
