LOCUS       sequence-46104-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 
            2.0 for ultrafast optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(E123A)-EYFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..48
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    99..132
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          172..375
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(195..215)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 taatagggcg cgccataact tcgtataatg tatgctatac gaagttatta agaggtttca
       61 tattgctaat agcagctaca atccagctac cattctgcat aacttcgtat aaagtatcct
      121 atacgaagtt attccggagt cgactctaga ggatccggta cctcacgaca cctgaaatgg
      181 aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa
      241 aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt cagtctccac
      301 ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta aagacgacgt
      361 actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact ccgtggagtc
      421 acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg actcacccgc
      481 ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc cgggaagcgg
      541 ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc agggcggggc
      601 gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag actacccccg
      661 tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc
      721 acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca gatcttggcc
      781 cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta
      841 aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgc
//
