LOCUS       sequence-461059-       12158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Partial ablation and subsequent lineage tracing of regenerating 
            hepatocytes in zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Eski SE, Mi J, Pozo-Morales M, Hovhannisyan GG, Perazzolo C, Manco 
            R, Ez-Zammoury I, Barbhaya D, Lefort A, Libert F, Marini F, Gurzov 
            EN, Andersson O, Singh SP
  TITLE     Cholangiocytes contribute to hepatocyte regeneration after partial 
            liver injury during growth spurt in zebrafish.
  JOURNAL   Nat Commun. 2025 Jun 6;16(1):5260. doi: 10.1038/s41467-025-60334-y.
  PUBMED    40480975
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-06-6"; volume: "16(1):5260. doi"; pages: " 
            10.1038/s41467-025-60334-y"
COMMENT     Sequence Label: 
            fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCh
            erry-NTR; cryaa:mCherry
FEATURES             Location/Qualifiers
     source          1..12158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(12..17,18..35,36..42)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    50..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     55..77
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     74..90
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     74..90
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     136..155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        136..154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2732..2765)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2774..2807)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2829..2876
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2877..3584
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             3585..3611
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     polyA_signal    3739..3860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3943..3976)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3996..4373
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     regulatory      4452..4461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4458..4460,4461..4463,4464..5177)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(4503..4524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    5298..5419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5426..5459)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5467..5476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5473..6180
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     regulatory      6226..6235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7010..7131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7650..7784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7802..7849
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    7873..8080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8069..8086
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8104..8814)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     protein_bind    complement(9380..9413)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(9432..9452)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9432..9450)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9483..9500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9483..9499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9492..9514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9640..10095)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9777..9798)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9989..10008
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10122..10226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10227..10295,10296..11087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10445..10464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11258..11846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11747..11766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12000..12017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12134..12155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
       61 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgt tagggataac
      121 agggtaatgc gcgcgtaata cgactcacta tagggcgaat tggagctctg cagaagagca
      181 tctctgaaca cacaacacgt ccaaccttga ggcagatggg ctacagcagc agaagaccac
      241 accgggtgcc gctcctgtca gctaagaaca ggaaactgag gctacaattc acacagactc
      301 accaaaactg gacgatatat tattagcccc ccttagaatt tttcatttga taatattttt
      361 cttctggcga aagcctcatt tgttttatat attatagaat aaaattagtt tttaatagtt
      421 tttatgccat tttaaggtca atattattag cccctttaag ctattttttt tcgatagtct
      481 acagaacaaa ccatcggtat acaatgactt gcctaattac cctaacctgc ctagttaccc
      541 taattaacct agttaagcct ttaaatgtca ctttaagctg tatagaagtg tcttgaagaa
      601 tatctagtct aatattattg actgtcatca tggcaaagat aaaataaatc agttattaaa
      661 actattatga ttagaaatgt gctgaaacaa tctgctctcc gataaacaga aattgaacaa
      721 aataaacagg ggggctaata aatttaaggg gttaaataat tctgattgca aaaaaaatga
      781 tgtctgcaaa actgttgcaa ataatttatt tgtgttgatt ttaagcaaac aaattaaatt
      841 taataaaata caacttaatc tgtttgttta aattcagccc taataaattg tttacagcca
      901 cttaacgtaa aaaaattgag taaatccaag gaatcatctc tgaataattt ttttcagtgt
      961 atatatatat atatatattc ttacaaaaca actcatttac tttagtttaa ttttcagggg
     1021 caaaaaacta aagtaatcga cgttgcttga ataaaaagtg taattaaggg aatgaggtaa
     1081 catttaacca tgtgtcaatg cagtttaaat atgccagtta gtggtatatg tttaaatggt
     1141 aagctattca aaactttaaa ctaacttaac cagccttttg ttgtcagact gaacagactt
     1201 tccatctgca ttattagaga ctaatctttg gctggatgaa tgattcatct gctgatattt
     1261 cagaatagac agattgaggc tgtttctaat atgattatgc aacctgaggg tgattatttg
     1321 aagcaaactc cacagaccag caggtcattg accgtcgtgt gttcaaacag agcagaaaca
     1381 tttgcaaaac tggtctgaca ggagaatcca gtccagcaca acacatatgc tgagcaaact
     1441 gaatcaatcc tgcaggtcaa ctctcgtgct ttaagtttat taagattatt ttatttattt
     1501 attattttat ttatctattt atttatttag ttgtttattt agcaattatt tgtttattta
     1561 tttgtttatt tattcctgca gatcatgcct tgtgcctttt tacatttaat ttaattttta
     1621 atttaatttc cttttacttt tttttatttt tttattttat tttattttac agtctgacaa
     1681 atactgaact aaaaaccctc agatcatgtc ttatgcattt cattttattt tatttcattt
     1741 tatattatta attttaatat ttttatttta cagtctgaca aatactgaat taaaaaccat
     1801 cagatcatgt ctcatgcatt taacttaact ttatttaatt caattaaatt gtttgtttgt
     1861 ttgtttcctt gcatttgttt gtttgttttt tacaatctga catactggac cgaaaaaact
     1921 cagatcatgt cttatgcatt ttattttatt ttatttttta ttttattgtc tattttattt
     1981 tatttcatct tattttattt tacagtctga catatggacc aataaatctc tcagatcatg
     2041 tctcatgcat ttaatttaat ttttttattt attcattttt atttatttat tttattattt
     2101 attttatttt aatatttatt ttattttatt ttattttatt ttacagtctg acatactgga
     2161 ccaataaatc tctcagatca tgtctcatgc attttacttt tattttatta gaattagaaa
     2221 gatcaaagga acaactttta aaatattaat tctgtatcaa aatctctttt gatacattta
     2281 attgatttaa aaaagcagtt cacccaagaa acatttcctc acagtcgaat ggttgtaaac
     2341 ttttatgaat tactttcaca gaaaaagatt tttggaagaa tattggaaaa aaagcagcca
     2401 ttgacttcca tagtaacaac aaaaaatact atggaagtca atggctgttt tttcaccatt
     2461 cggtatcttc attctggagc agaatttttt gggtgatctg tccctttaag tcgtcaaatc
     2521 ctggtgcaat attccacatg cactgacgag tctttatttt tggtctgcta ctgctgtgtg
     2581 tgtgagggca tttacctcat cttctgctgg agttgatgaa cggtgggttg ttcaaacagc
     2641 agcaggtcat tgactgaact cctctcgata taaaagctgc agatctgaag ctgaccttca
     2701 ctttgtgttg atcgctagcg aattcgatct cataacttcg tataggatac tttatacgaa
     2761 gttataactc gagataactt cgtatagcat acattatacg aagttatacc ggcagatctg
     2821 ccaccatgaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggtgt
     2881 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     2941 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     3001 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     3061 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     3121 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     3181 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     3241 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     3301 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     3361 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     3421 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     3481 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     3541 ccagatactg cgacctccct agcaaactgg ggcacaagct taatccagca gcgaagaaaa
     3601 agaagctgga ttaaagtgtg cggccgactc tagatcataa tcagccatac cacatttgta
     3661 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3721 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3781 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3841 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatgctccga gggccgccac
     3901 cacctgttcc tgtaggaatt ggccgcgact ctaggatcta ggataacttc gtataggata
     3961 ctttatacga agttattgat ccaccggtcg ccaccatgcc agagccagcg aagtctgctc
     4021 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
     4081 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
     4141 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaactcg tttgtgaacg
     4201 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
     4261 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
     4321 agcacgccgt gtccgagggt actaaggccg tcaccaagta caccagcgct aagggatctg
     4381 ggggtggagg ctccggcggg ggtggatctg gaggtggggg cagcggcgga ggtgggctag
     4441 cgctaccgcg ggccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4501 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     4561 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4621 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     4681 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4741 aggagcgcac catctctttc aaggacgacg gaacctacaa gacccgcgcc gaggtgaagt
     4801 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     4861 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     4921 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     4981 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5041 tgctgcccga caaccactac ctgagccatc agtccaaact gagcaaagac cccaacgaga
     5101 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     5161 acgagctgta caagtaaagc ggccgactct agatcataat cagccatacc acatttgtag
     5221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5341 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5401 aactcatcaa tgtatcttaa tcgagataac ttcgtatagc atacattata cgaagttata
     5461 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     5521 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     5581 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     5641 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     5701 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5761 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5821 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5881 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5941 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     6001 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     6061 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6121 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     6181 ggatccctca gatcacgtgc tcaagcatcg aactcaggcg gcggcgccgc catggacatc
     6241 atcagcgtgg ctctgaagag gcactccacc aaggctttcg acgcttccaa gaaactgacc
     6301 cctgaacagg ccgagcagat caagaccctg ctccagtaca gccctagctc caccaacagc
     6361 cagccttggc acttcatcgt ggctagcacc gaggaaggca aagctagggt ggctaagagc
     6421 gccgctggca actacgtgtt caacgagagg aagatgctgg atgctagcca cgtggtggtg
     6481 ttctgcgcta agaccgccat ggacgatgtg tggctgaagc tggtggtgga tcaggaagat
     6541 gctgatggca ggttcgctac ccctgaagct aaggccgcta acgacaaggg caggaagttc
     6601 ttcgccgaca tgcacaggaa ggatctgcac gatgatgctg agtggatggc caagcaggtg
     6661 tacctgaacg tgggcaactt cctgctcggc gtggctgccc tgggcctcga tgctgtgccc
     6721 atcgaaggct tcgatgctgc tatcctggat gccgagttcg gcctgaagga gaaaggctac
     6781 accagcctgg tggtggtgcc tgtgggccac cacagcgtgg aggacttcaa cgctaccctg
     6841 cctaagagca ggctgcccca gaacatcacc ctgaccgagg tgtgaagcgg cctagggact
     6901 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6961 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     7021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt ataggcgtaa
     7141 attgtaagcg ttaattcgag gtccttcctc tgcagaggtc ttgcttctcc cggtcagctg
     7201 actccctccc caagtccttc aaatatctca gaacatgggg agaaacgggg accttgtccc
     7261 tcctaaggaa ccccagtgct gcatgccatc atccccccca ccctcgcccc cacccccgcc
     7321 acttctccct ccatgcatgt cgacttaatt aagcggccgc cgaaatgacc gaccaagcga
     7381 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7441 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7501 agttcttcgc ccaccccccg gatctaagct ctagataagt aatgatcata atcagccata
     7561 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     7621 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     7681 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7741 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggggg taccgcgtcg
     7801 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg ggcccgcggt
     7861 ggcggcgatc tctccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc
     7921 tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc
     7981 tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg
     8041 gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggcc
     8101 gctttacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
     8161 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt
     8221 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc
     8281 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
     8341 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
     8401 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
     8461 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
     8521 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
     8581 cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag
     8641 ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
     8701 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
     8761 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccatgttgtc
     8821 agacctggta actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa
     8881 cgcaagtagg aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg
     8941 gccttagtgg aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa
     9001 tggatctctc tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg
     9061 gggtggactc tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt
     9121 ggccaactat caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca
     9181 tggctgtaga acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc
     9241 taattttttt aaatatcaca gataatcaat cgcttttttt ttttcttgac tgaaaagctt
     9301 agactggaaa attctttgaa aaagtttgca ctacagaaaa taaatacata acatcctgca
     9361 ctgaatgcac actattaatg aagttcctat actttctaga gaataggaac ttcggtaccc
     9421 agcttttgtt ccctttagtg agggttaatt gcgcgctagg gataacaggg taatacgcgc
     9481 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9541 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     9601 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt
     9661 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     9721 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     9781 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     9841 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     9901 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     9961 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    10021 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    10081 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10141 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10201 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10261 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10321 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10381 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10441 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10501 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10561 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10621 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10681 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10741 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10801 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10861 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10921 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10981 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11041 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11101 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11161 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11221 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11281 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11341 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11401 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11461 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11521 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11581 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11641 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11701 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11761 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11821 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11881 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11941 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12001 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12061 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12121 agcgcaacgc aattaatgtg agttagctca ctcattag
//
