LOCUS       sequence-46106-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 
            2.0 for ultrafast optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(E123A)-mCherry
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..79)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(130..163)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      168..177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttgtaatcc agaggttgat tatcgataag cttgatatcg aattcataac ttcgtatagc
       61 atacattata cgaagttatt ctttgcacca ttctaaagaa taacagtgat aatttctggg
      121 ttaaggcaaa taacttcgta taggatactt tatacgaagt tatgctagcc accatggact
      181 atggcggcgc tttgtctgcc gtcggacgcg aacttttgtt cgttactaat cctgtggtgg
      241 tgaacgggtc cgtcctggtc cctgaggatc aatgttactg tgccggatgg attgaatctc
      301 gcggcacgaa cggcgctcag accgcgtcaa atgtcctgca gtggcttgca gcaggattca
      361 gcattttgct gctgatgttc tatgcctacc aaacctggaa atctacatgc ggctgggagg
      421 agatctatgt gtgcgccatt gaaatggtta aggtgattct cgagttcttt tttgagttta
      481 agaatccctc tatgctctac cttgccacag gacaccgggt gcagtggctg cgctatgcag
      541 cctggctgct cacttgtcct gtcatcctta tccacctgag caacctcacc ggcctgagca
      601 acgactacag caggagaacc atgggactcc ttgtctcaga catcgggact atcgtgtggg
      661 gggctaccag cgccatggca accggctatg ttaaagtcat cttcttttgt cttggattgt
      721 gctatggcgc gaacacattt tttcacgccg ccaaagcata tatcgagggt tatcatactg
      781 tgccaaaggg tcggtgccgc caggtcgtga ccggcatggc atggctgttt ttcgtgagct
      841 ggggtatgtt cccaattctc ttcattttgg ggcccgaagg ttttggcgtc
//
