LOCUS       sequence-461060-        9268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible Cre expression in Zebrafish hepatocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Eski SE, Mi J, Pozo-Morales M, Hovhannisyan GG, Perazzolo C, Manco 
            R, Ez-Zammoury I, Barbhaya D, Lefort A, Libert F, Marini F, Gurzov 
            EN, Andersson O, Singh SP
  TITLE     Cholangiocytes contribute to hepatocyte regeneration after partial 
            liver injury during growth spurt in zebrafish.
  JOURNAL   Nat Commun. 2025 Jun 6;16(1):5260. doi: 10.1038/s41467-025-60334-y.
  PUBMED    40480975
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-06-6"; volume: "16(1):5260. doi"; pages: " 
            10.1038/s41467-025-60334-y"
COMMENT     Sequence Label: fabp10a:CreERT2; cryaa:mCerulean
FEATURES             Location/Qualifiers
     source          1..9268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..1040
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1053..1988
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1101..1994
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    2124..2245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2161..2180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2215..2234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    2288..2495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2484..2501
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2519..3232,3233..3235,3236..3238))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2564..2585)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2913..2932
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3822..3842)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3822..3840)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3873..3890)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3873..3889)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3882..3904)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4030..4485)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4167..4188)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4379..4398
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4512..4616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4617..4685,4686..5477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4835..4854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5648..6236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6137..6156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6390..6407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6524..6545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6560..6565,6566..6583,6584..6590)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6598..6614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6603..6625
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6622..6638
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6622..6638
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6684..6703
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6684..6702
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattcgccac catgtccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg
       61 atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt
      121 tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca
      181 agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat
      241 atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca
      301 tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta
      361 tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt
      421 tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg
      481 atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa
      541 ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata
      601 ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg
      661 taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact
      721 acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat
      781 caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg
      841 atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc
      901 gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca
      961 atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc
     1021 gcctgctgga agatggcgat ctcgagccat ctgctggaga catgagagct gccaaccttt
     1081 ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg tccctgacgg
     1141 ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat tccgagtatg
     1201 atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac ctggcagaca
     1261 gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg gatttgaccc
     1321 tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg attggtctcg
     1381 tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg ctcttggaca
     1441 ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg ctggctacat
     1501 catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc aaatctatta
     1561 ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct ctggaagaga
     1621 aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac ctgatggcca
     1681 aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc ctcatcctct
     1741 cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg aagtgcaaga
     1801 acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc
     1861 ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg gccactgcgg
     1921 gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca gagggtttcc
     1981 ctgccacagc ttgattaatt aagcggccgc gactctagat cataatcagc cataccacat
     2041 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2101 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2161 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2221 tgtccaaact catcaatgta tcttaatcga gggggggccc ggtacccacc gcggtggcgg
     2281 cgatctctcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
     2341 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
     2401 ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg
     2461 gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc gggccgcttt
     2521 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     2581 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt
     2641 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     2701 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
     2761 tcttctgctt gtcggcggtg atatagacgt tgtcgctgat ggcgttgtac tccagcttgt
     2821 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     2881 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     2941 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     3001 tcatgtggtc ggggtagcgg gcgaagcact gcacgcccca ggtcagggtg gtcacgaggg
     3061 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     3121 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     3181 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgt
     3241 tgtcagacct ggtaactcct tactgtaact tattgcacct gatgaacaaa agatgtgaac
     3301 tgaacgcaag taggaggcta atagaggcta gtacagtaca aagatcccca aatgatgtgt
     3361 gccggcctta gtggaggcag acccctggtt atatacctgt gctgcagaaa gaggggctta
     3421 caaatggatc tctctggaat ggcattatct catggtggga acagtgttgt cagcagaatc
     3481 agcggggtgg actctgacct gagaatgaca gtctagcact gaatggctca gacaatactc
     3541 ttgtggccaa ctatcaccgg aaaataagtg cctgccaaaa caaaccagac cagatgaagc
     3601 tacatggctg tagaacgtca gtatttaatg tgttgtggcc acttggttca ccagatttaa
     3661 gagctaattt ttttaaatat cacagataat caatcgcttt ttttttttct tgactgaaaa
     3721 gcttagactg gaaaattctt tgaaaaagtt tgcactacag aaaataaata cataacatcc
     3781 tgcactgaat gcacactatt aatggtaccc agcttttgtt ccctttagtg agggttaatt
     3841 gcgcgctagg gataacaggg taatacgcgc tcactggccg tcgttttaca acgtcgtgac
     3901 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3961 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4021 ggcgaatgga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4081 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4141 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4201 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4261 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4321 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4381 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4441 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     4501 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4561 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4621 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4681 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4741 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4801 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4861 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4921 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4981 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5041 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5101 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5161 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5221 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5281 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5341 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5401 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5461 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5521 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5581 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5641 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5701 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5761 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5821 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5881 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5941 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6001 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6061 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6121 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6181 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6241 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6301 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6361 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6421 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6481 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6541 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6601 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgtta
     6661 gggataacag ggtaatgcgc gcgtaatacg actcactata gggcgaattg gagctctgca
     6721 gaagagcatc tctgaacaca caacacgtcc aaccttgagg cagatgggct acagcagcag
     6781 aagaccacac cgggtgccgc tcctgtcagc taagaacagg aaactgaggc tacaattcac
     6841 acagactcac caaaactgga cgatatatta ttagcccccc ttagaatttt tcatttgata
     6901 atatttttct tctggcgaaa gcctcatttg ttttatatat tatagaataa aattagtttt
     6961 taatagtttt tatgccattt taaggtcaat attattagcc cctttaagct attttttttc
     7021 gatagtctac agaacaaacc atcggtatac aatgacttgc ctaattaccc taacctgcct
     7081 agttacccta attaacctag ttaagccttt aaatgtcact ttaagctgta tagaagtgtc
     7141 ttgaagaata tctagtctaa tattattgac tgtcatcatg gcaaagataa aataaatcag
     7201 ttattaaaac tattatgatt agaaatgtgc tgaaacaatc tgctctccga taaacagaaa
     7261 ttgaacaaaa taaacagggg ggctaataaa tttaaggggt taaataattc tgattgcaaa
     7321 aaaaatgatg tctgcaaaac tgttgcaaat aatttatttg tgttgatttt aagcaaacaa
     7381 attaaattta ataaaataca acttaatctg tttgtttaaa ttcagcccta ataaattgtt
     7441 tacagccact taacgtaaaa aaattgagta aatccaagga atcatctctg aataattttt
     7501 ttcagtgtat atatatatat atatattctt acaaaacaac tcatttactt tagtttaatt
     7561 ttcaggggca aaaaactaaa gtaatcgacg ttgcttgaat aaaaagtgta attaagggaa
     7621 tgaggtaaca tttaaccatg tgtcaatgca gtttaaatat gccagttagt ggtatatgtt
     7681 taaatggtaa gctattcaaa actttaaact aacttaacca gccttttgtt gtcagactga
     7741 acagactttc catctgcatt attagagact aatctttggc tggatgaatg attcatctgc
     7801 tgatatttca gaatagacag attgaggctg tttctaatat gattatgcaa cctgagggtg
     7861 attatttgaa gcaaactcca cagaccagca ggtcattgac cgtcgtgtgt tcaaacagag
     7921 cagaaacatt tgcaaaactg gtctgacagg agaatccagt ccagcacaac acatatgctg
     7981 agcaaactga atcaatcctg caggtcaact ctcgtgcttt aagtttatta agattatttt
     8041 atttatttat tattttattt atctatttat ttatttagtt gtttatttag caattatttg
     8101 tttatttatt tgtttattta ttcctgcaga tcatgccttg tgccttttta catttaattt
     8161 aatttttaat ttaatttcct tttacttttt tttatttttt tattttattt tattttacag
     8221 tctgacaaat actgaactaa aaaccctcag atcatgtctt atgcatttca ttttatttta
     8281 tttcatttta tattattaat tttaatattt ttattttaca gtctgacaaa tactgaatta
     8341 aaaaccatca gatcatgtct catgcattta acttaacttt atttaattca attaaattgt
     8401 ttgtttgttt gtttccttgc atttgtttgt ttgtttttta caatctgaca tactggaccg
     8461 aaaaaactca gatcatgtct tatgcatttt attttatttt attttttatt ttattgtcta
     8521 ttttatttta tttcatctta ttttatttta cagtctgaca tatggaccaa taaatctctc
     8581 agatcatgtc tcatgcattt aatttaattt ttttatttat tcatttttat ttatttattt
     8641 tattatttat tttattttaa tatttatttt attttatttt attttatttt acagtctgac
     8701 atactggacc aataaatctc tcagatcatg tctcatgcat tttactttta ttttattaga
     8761 attagaaaga tcaaaggaac aacttttaaa atattaattc tgtatcaaaa tctcttttga
     8821 tacatttaat tgatttaaaa aagcagttca cccaagaaac atttcctcac agtcgaatgg
     8881 ttgtaaactt ttatgaatta ctttcacaga aaaagatttt tggaagaata ttggaaaaaa
     8941 agcagccatt gacttccata gtaacaacaa aaaatactat ggaagtcaat ggctgttttt
     9001 tcaccattcg gtatcttcat tctggagcag aattttttgg gtgatctgtc cctttaagtc
     9061 gtcaaatcct ggtgcaatat tccacatgca ctgacgagtc tttatttttg gtctgctact
     9121 gctgtgtgtg tgagggcatt tacctcatct tctgctggag ttgatgaacg gtgggttgtt
     9181 caaacagcag caggtcattg actgaactcc tctcgatata aaagctgcag atctgaagct
     9241 gaccttcact ttgtgttgat cgctagcg
//
