LOCUS       sequence-46108-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of EF1a-driven, Cre-dependent, humanized 
            channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic 
            activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..85)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(136..169)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccaattttg taatccagag gttgattatc gataagcttg atatcgaatt cataacttcg
       61 tatagcatac attatacgaa gttattcttt gcaccattct aaagaataac agtgataatt
      121 tctgggttaa ggcaaataac ttcgtatagg atactttata cgaagttatg ctagccacca
      181 tggactatgg cggcgctttg tctgccgtcg gacgcgaact tttgttcgtt actaatcctg
      241 tggtggtgaa cgggtccgtc ctggtccctg aggatcaatg ttactgtgcc ggatggattg
      301 aatctcgcgg cacgaacggc gctcagaccg cgtcaaatgt cctgcagtgg cttgcagcag
      361 gattcagcat tttgctgctg atgttctatg cctaccaaac ctggaaatct acatgcggct
      421 gggaggagat ctatgtgtgc gccattgaaa tggttaaggt gattctcgag ttcttttttg
      481 agtttaagaa tccctctatg ctctaccttg ccacaggaca ccgggtgcag tggctgcgct
      541 atgcaacctg gctgctcact tgtcctgtca tccttatcca cctgagcaac ctcaccggcc
      601 tgagcaacga ctacagcagg agaaccatgg gactccttgt ctcagacatc gggtgtatcg
      661 tgtggggggc taccagcgcc atggcaaccg gctatgttaa agtcatcttc ttttgtcttg
      721 gattgtgcta tggcgcgaac acattttttc acgccgccaa agcatatatc gagggttatc
      781 atactgtgcc aaagggtcgg tgccgccagg tcgtgaccgg catggcatgg ctgtttttcg
      841 tgagctgggg tatgttccca attctcttca ttttggggcc cgaaggtttt ggcgtcctga
      901 gcgtctatgg
//
