LOCUS       sequence-46109-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for 
            ultrafast optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    236..269
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    320..353
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          393..596
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(416..436)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
       61 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
      121 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
      181 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatagggc gcgccataac
      241 ttcgtataat gtatgctata cgaagttatt aagaggtttc atattgctaa tagcagctac
      301 aatccagcta ccattctgca taacttcgta taaagtatcc tatacgaagt tattccggag
      361 tcgactctag aggatccggt acctcacgac acctgaaatg gaagaaaaaa actttgaacc
      421 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
      481 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
      541 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
      601 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
      661 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
      721 gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
      781 actggtgccg accgggccag ccttgccgcc cagggcgg
//
