LOCUS       sequence-461093-       13325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Tol2 based expression vector with the Hsp70 promoter driving a cre
            dependent switch reporter. Contains an endothelial driven cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13325)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 13325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: 
            pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)
FEATURES             Location/Qualifiers
     source          1..13325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(667..678,679..841)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1245..1265
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2785..2809
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2810..2843)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2857..2875
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2857..2874
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2980..3357
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3370..3379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3376..3378,3379..3381,3382..4095)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3421..3442)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3682..3701)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4029..4050
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4131..4265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4381..4515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4601..4619)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4640..4656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4664..4680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(4688..4721)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(4722..4746)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4763..4772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4772..4813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4829..5524
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4962..4980)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5230..5249
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5592..5726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5789..5809)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5817..5833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5853..5934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6458..6592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6614..6634)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6659..7357)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     intron          8183..8315
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(8550..8570)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8573..8582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10913..11100,11101..11112)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        complement(11137..11155)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11176..11192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11200..11216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11224..11230,11231..11248,11249..11254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11269..11290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11407..11424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11578..12166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11658..11677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12337..13128,13129..13197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12960..12979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13198..13302)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 aattaacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      721 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      781 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      841 aagaaaacta gagattcttg tttaagctat ttaatctcaa aaaacattaa atgaaatgca
      901 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      961 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     1021 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     1081 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     1141 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1201 gatcctaggg ggcccgttta aacgccatga ttacgccaag ctatcaactt tgtatagaaa
     1261 agttgcttca ggggtgtcgc ttggttattt ccaaaaatca aattaatttt attaaactat
     1321 tagaacgagc atgttttgtc tatatgctac agaagataaa aaataatagg agttaacagt
     1381 tataaaacaa cacactttgt ttctattgat tgttgaccac actggggtct cattaagtta
     1441 gattaaagac acactaactg ggtcaaaagc agcagattga tttcatagca ccagggtaaa
     1501 ctttctaaca cttttacggc aatcatatac attaaaatta aatacagacc acgactgaac
     1561 aaggaggatg atctccaata ttaaacaaag agacttgtgc ctatttctct gagggtaaac
     1621 atgacctctc aagttagcaa gttggtttta acactacaaa aatagttaag actgcaatcc
     1681 cagaataaag tattggtttt aaccaatcaa tatagtacag taaacatcca tttgttttgt
     1741 tgaaacgtta aacaaatctg accaaagcta ttagcttata taaaacaggt ttgccttcta
     1801 tgtagctgaa aacaccacag gcccgatttt gctactgtgt aaaacatttc agcaagattt
     1861 ttttattgca ttttttttta ctgaatcgtt caaacatttt atcattttag tttgttcatt
     1921 cattgcaact ggaaaaacaa cacatcacac aaccgcacat atttcagcaa taagtacaat
     1981 aaaacactca aataaaaaaa acattttaaa tctctttgta tttttgaccg ctgtttcgcg
     2041 taatttcacg gtaaaactct ggaaatctcc actacattcc tctcagcggc tcctctcaat
     2101 gacagctgaa gaagtgacgc ggctgcctgc tgtgttttga ttggtcgaat tcactggagg
     2161 cttccagaac agtgtagagt ctgaacgggt gcgcgctctg ctgtatttaa agggcgaaag
     2221 agagaccgca gagaaactca accgaagaga agcgacttga caaagaagaa aagagcagcc
     2281 tgacaggact ttttccccga cgaggtgttt attcgctcta tttaagaatc tactgtaagg
     2341 taagtctcaa tatattgtac tctattggct aatcagaatt atatagagat tatatgtact
     2401 taatgtcaaa aaatcaactt tgtatatgta atctttttac atgtggactg cctatgttca
     2461 tcttatttta ggtctactag aaaattatat ttcccgtttt cacaataagg atttttaaaa
     2521 aaagcaatga acagacgggc atttacttta tgttgctgac attattttat atgagcataa
     2581 taaccataaa tactagcaaa tgtcctaaat gaatttgtgt taatgttgtc tacaaaagaa
     2641 aattagcgtt ttacttgtac aactaataat aacttggtta ttaagagaat ttcacttgtt
     2701 gactagaaaa atcctttcat aatgaaacaa ttgcaccata aattgtataa atataaaatt
     2761 aattctaatt gttttttttt cctgccaagt ttgtacaaaa aagcaggcta taacttcgta
     2821 tagcatacat tatacgaagt tatcggagca agcttgattt aggtgacact atagaataca
     2881 agctacttgt tctttttgca ggatcccatc gattcgaatt caaggcctct cgagcctcta
     2941 gaactatagt gagtcgtatt actcgacggt accgccacca tgccagagcc agcgaagtct
     3001 gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc
     3061 aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag
     3121 caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg
     3181 aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc
     3241 tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg
     3301 gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaaggat
     3361 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3421 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3481 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3541 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cgcccgctac
     3601 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3661 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3721 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3781 aacatcctgg ggcacaagct ggagtacaac gccatcagcg acaacgtcta tatcaccgcc
     3841 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     3901 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3961 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     4021 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4081 gagctgtaca agtaaagcgg ccctagaact atagtgagtc gtattacgta gatccagaca
     4141 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4201 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4261 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4321 ttttttaatt cgcggccgca agtaaagcgg ccctagaact atagtgagtc gtattacgta
     4381 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4441 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4501 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4561 gtgtgggagg ttttttgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     4621 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4681 ttccacaata acttcgtata gcatacatta tacgaagtta tacccagctt tcttgtacaa
     4741 agtgggatcc actagtgcta gcgccaccat gggtaagcct atccctaacc ctctcctcgg
     4801 tctcgattct acgggtggtg ctggtggtat ggtgagcaag ggcgaggcag tgatcaagga
     4861 gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga
     4921 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4981 gggtggcccc ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag
     5041 ggccttcatc aagcaccccg ccgacatccc cgactactat aagcagtcct tccccgaggg
     5101 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gccgtgaccg tgacccagga
     5161 cacctccctg gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc
     5221 tcctgacggc cccgtaatgc agaagaagac aatgggctgg gaagcatcca ccgagcggtt
     5281 gtaccccgag gacggcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg
     5341 cggccgctac ctggcggact tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc
     5401 cggcgcctac aacgtcgacc gcaagttgga catcacctcc cacaacgagg actacaccgt
     5461 ggtggaacag tacgaacgct ccgagggccg ccactccacc ggcggcatgg acgagctgta
     5521 caagtaaggc gcgccctcga gctcaagctt cgaattctgc agtctagaac tatagtgagt
     5581 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     5641 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5701 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     5761 ttcaggggga ggtgtgggag gttttttaca actttattat acatagttga taattcactg
     5821 gccgtcgttt tacggtacca tcgatgatga tcaacttgtt tattgcagct tataatggtt
     5881 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcgatatc
     5941 aatcgggaca gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg
     6001 gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag
     6061 ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg
     6121 aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt
     6181 aggctgagtg cacggggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     6241 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     6301 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     6361 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     6421 aaaaagcttt aggctgatac gtagtagatg cggccgcaac ttgtttattg cagcttataa
     6481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgcgatcgc
     6601 gtctagatta tcataccttt ctcttctttt ttggaccacc agcaccacct cctcgagcat
     6661 taagcttgtg ccccagtttg ctagggaggt cgcagtatct ggccactgcc acctcgtgct
     6721 gctcgacgta ggtctcgttg ttggcctcct tgattctttc cagtctgtag tccacatagt
     6781 agacgccagg catcttgagg ttcttagcgg gtttcttgga tctatatgtg gtcttggcgt
     6841 ttgcgatcag atggctcccg cccacgagct tcagggccat gtcgtttctg ccttccaggc
     6901 cgccgtcagc ggggtacagc gtctcggtga aggcctccca gccgagtgtt ttcttctgca
     6961 tcacagggcc gttggatgtg aagttcaccc ctctgatctt gacgttgtag atgaggcagc
     7021 cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc cccgtcttcg tatgtggtga
     7081 ctctctccca tgtgaagccc tcagggaagg actgcttgaa gaagtcgggg atgccctggg
     7141 tgtggttgat gaaggtcttg ctgccgtaga ggaagctagt agccaggatg tcgaaggcga
     7201 aggggagagg gccgccctcg accaccttga ttctcatggt ctgggtgccc tcgtagggct
     7261 tgccttcgcc ctcggatgtg cacttgaagt gatggttgtc cacggtgccc tccatgtaca
     7321 gcttcatgtg catgttctcc ttaatcagct cgctcatggt ggatccggcc ccgccggctc
     7381 cgccgctagc gtctccatcc tccagcagtc tcaccatagc tcctgtctca gagtccaggt
     7441 ttctgatgta gttcatcaca atgttcacat ttgtccatcc tccagcctgc atgatctcag
     7501 ggatgctcac tccagctcta gccatgtctc tagcagctcc cactctagca gagtgtccag
     7561 accaagccag gtatctctgt ccagagtcat ccttagctcc atagatcagt ctgtgtgtag
     7621 cctcaaagat tccctccaga gcccttgtgc tcagctggct tgtagcagaa ggagcagcca
     7681 ctccattctt tctcactcta cagaacaggt agttgttagg gtcatcagcc actccagaca
     7741 cagagatcca tctctccacc agctttgtca ctcccaggct cagagccttc tccactccag
     7801 ctgtgctcac cagtgtcttt gttcttccaa tgtggatcag cattcttcct ccatctgttc
     7861 tgctgatgtc cttcactctg attctagcaa tctcagcaat tctcagcagt gtgttgtaag
     7921 caattcccag gaaagccagg tttctgatgt cctgacatct gtcagagttc tccatcaggc
     7981 ttctcacctg gtcaaagtct gttctctcaa aagccagagc ctgcttagct ctctctccag
     8041 catccacatt ctcctttctg attcttctca tcaccaggct cacagcattg ctgtcagaag
     8101 gtctaggcag tccagacctt ctgtgcagca tgttcagctg tcccaggtgc tgctggattg
     8161 tcttcacagc cagtcctcta gcctgtggag agaaaggcaa agtggatgtc agtaagacca
     8221 ataggtgcct atcagaaacg caagagtctt ctctgtctcg acaagcccag tttctattgg
     8281 tctccttaaa cctgtcttgt aaccttgata cttacctgga ggtacagcag gtagtctctc
     8341 acatcctcag gctcagcagg gaaccacttt ctgttgttca gcttacacca agcagcccag
     8401 ctcctgcaga cagacagcag catcttccat gtgtgctcag agaaagcctg tctgtctctg
     8461 aacatgtcca tcaggttctt tctcacctca tcagatgtag catccacagg cagagcaggt
     8521 agattttggt ggacggttag aagattggag actttcctct tcttcttggg caccatggtg
     8581 gccatatgcg gcacgcgtcg gatggttttt tttcctctaa atttgggaag tgaagtcact
     8641 gcgcttgaga aggaagccgt gtatgcgtgt atgtgtgtgt gtgtatgtgt gtgtgtcact
     8701 ggccctgcct tcattacggc acccaaagca ggatatttgt ccagcgaaga taagacacca
     8761 aaacaatggt gtttctttcc tttgtgcatg tttttgcctg gaaatattta cagaatgcct
     8821 gccaactctt tacatttgtt gcgctttttt tccgacatta aacaaggcgg gtttgtagaa
     8881 acattgcatt gatgcatcaa agctgtgcta gaaataatca ttaatacaac gtctgaaata
     8941 acagattatg ctgattaata taggctaacc atagtttaag gctaaaataa aatatatact
     9001 ttttcaacga tcgaatacaa atatgtctaa tgcactcggt ctaataataa tctcataata
     9061 tgatgaatat tgttccgtta tatccaataa ctttgatttg atctacctat ggtgaagtga
     9121 agaatgtgac gagtctgccc gagcttcttc taaaatgggc ttccatgaat aaaaagcaaa
     9181 gcccgctgcg tcgagtctgt ctgctccaca cgtgtgcgcg cctctgaatg cacatgaacg
     9241 cagcgagcct ggtgctgtat ggaccatata gagaggctga tacctcttgt gtgctgtgct
     9301 gttcaacatt tctttaaatc agtagcatat gacctcacat ttttcatgga caataatagg
     9361 aatattgtgc gtgttagact acatttactg tgcattaaat gtctcatttc gttaaaaaaa
     9421 gaaaaataga tatatttccg acttgtagtg tttagtgtcg aaacatattt tgcatgcatt
     9481 aaatgcctgc tatttttata tagcatatac agcgtgcatg tgattccatc tctaaatcca
     9541 aaatctccac cccctctttt tcagttaccg agcacgcatt cttggtacat tgaggtcgaa
     9601 atgctcacaa agctgctatt ttcaacatgg ctataaattg gccgtgtctg caaggctcgc
     9661 ttaaaccatc cagaaccgca ggattacgct ggaatagaga ttttacgctg aggtgtgtca
     9721 agagctcatc tttgtactgc tgaatatgct gcatgatgaa taatgcatgt atgctaccgt
     9781 gcttttaact taacaggggg acaaaaaact gttttcataa gccaacttga tcgtgattgg
     9841 ctgtcccgca gaagatgcgt catgaggtcg ggatgtacag agacccatta aaagcgctgt
     9901 gctattttat acagagggca ttcctgtctt ttgtttcagg aggaaaagga aacctgacag
     9961 gaaaccagat tgtgtggaac aggaaatacc catagattga gacacccttc tcattgtcga
    10021 ctaaagtgta cagttaaaag tcaatcatgt gaggtatctg tgtcaaattg tgaaggaaaa
    10081 aacatgcagg ttactattat aatctgtgga tgcatatgtt tactacactg aaaaacccat
    10141 aaagttatgg aaactcaaac cgtttgagga aaccgactgc aacaaaccat ttaagttcaa
    10201 aaactaatcc taatgagtac tgtgaactta atctatttga ttaaacgatg caatttgagc
    10261 acatgttgaa cccaataagt gaagtgaact caaaccaact gagtactgta aaacccaaaa
    10321 agttaaggca actcaaactg cttgagggaa ccgactgcta caaaccattt gagctaaact
    10381 aatctatgtg agtaccatga acttactcca gttaagttga agtaatgagg tatttaatga
    10441 actcactacc ttcaacactg agtttaaaac tcttttcgaa tgagtagaat caacattcag
    10501 taaattttga gttaattaca ctcatttcat ttgataaagt tgactgttgg gttttagtgt
    10561 atgtttatgg gtcaagatga gatactagta tcgatgcgaa gatacggcca cgggtgctct
    10621 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
    10681 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
    10741 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
    10801 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
    10861 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
    10921 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
    10981 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
    11041 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
    11101 tttacacctc tgagatctga gctccagctt ttgttccctt tagtgagggt taattgcgcg
    11161 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    11221 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    11281 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    11341 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    11401 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    11461 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    11761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    11821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    11881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    11941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12001 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    12301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    12361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    12421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    12781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    12841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    12901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    12961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    13021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    13081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    13141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    13201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    13261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    13321 gccac
//
