LOCUS       sequence-461094-       13178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A Tol2 based expression vector containing fli1 driven switch 
            reporter (loxP flanked AmCyan followed by a mScarlet-I) and a 
            hsp70-TagBFP-zCre in the opposite direction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 13178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-...
FEATURES             Location/Qualifiers
     source          1..13178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(667..678,679..841)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1245..1265
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3308..3332
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3333..3366)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3397..3406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3403..4092
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan1
                     /note="mammalian codon-optimized"
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     intron          4178..4243
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4373..4393
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4818..4952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5007..5040)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5041..5065)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5082..5091
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5091..5132
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5148..5843
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5281..5299)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5549..5568
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5911..6045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6108..6128)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6136..6152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6172..6253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6777..6911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6933..6953)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6978..7676)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     intron          8502..8634
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(8869..8889)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8892..8901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10766..10953,10954..10965)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10990..11010)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10990..11008)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11029..11045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11029..11045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11042..11064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11053..11069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11077..11083,11084..11101,11102..11107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11122..11143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11260..11277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11431..12019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11511..11530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12190..12981,12982..13050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12813..12832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13051..13155)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 aattaacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      721 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      781 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      841 aagaaaacta gagattcttg tttaagctat ttaatctcaa aaaacattaa atgaaatgca
      901 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      961 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     1021 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     1081 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     1141 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1201 gatcctaggg ggcccgttta aacgccatga ttacgccaag ctatcaactt tgtatagaaa
     1261 agttggatct catcttgacc cataaacata cactaaaacc caacagtcaa ctttatcaaa
     1321 tgaaatgagt gtaattaact caaaatttac tgaatgttga ttctactcat tcgaaaagag
     1381 ttttaaactc agtgttgaag gtagtgagtt cattaaatac ctcattactt caacttaact
     1441 ggagtaagtt catggtactc acatagatta gtttagctca aatggtttgt agcagtcggt
     1501 tccctcaagc agtttgagtt gccttaactt tttgggtttt acagtactca gttggtttga
     1561 gttcacttca cttattgggt tcaacatgtg ctcaaattgc atcgtttaat caaatagatt
     1621 aagttcacag tactcattag gattagtttt tgaacttaaa tggtttgttg cagtcggttt
     1681 cctcaaacgg tttgagtttc cataacttta tgggtttttc agtgtagtaa acatatgcat
     1741 ccacagatta taatagtaac ctgcatgttt tttccttcac aatttgacac agatacctca
     1801 catgattgac ttttaactgt acactttagt cgacaatgag aagggtgtct caatctatgg
     1861 gtatttcctg ttccacacaa tctggtttcc tgtcaggttt ccttttcctc ctgaaacaaa
     1921 agacaggaat gccctctgta taaaatagca cagcgctttt aatgggtctc tgtacatccc
     1981 gacctcatga cgcatcttct gcgggacagc caatcacgat caagttggct tatgaaaaca
     2041 gttttttgtc cccctgttaa gttaaaagca cggtagcata catgcattat tcatcatgca
     2101 gcatattcag cagtacaaag atgagctctt gacacacctc agcgtaaaat ctctattcca
     2161 gcgtaatcct gcggttctgg atggtttaag cgagccttgc agacacggcc aatttatagc
     2221 catgttgaaa atagcagctt tgtgagcatt tcgacctcaa tgtaccaaga atgcgtgctc
     2281 ggtaactgaa aaagaggggg tggagatttt ggatttagag atggaatcac atgcacgctg
     2341 tatatgctat ataaaaatag caggcattta atgcatgcaa aatatgtttc gacactaaac
     2401 actacaagtc ggaaatatat ctatttttct ttttttaacg aaatgagaca tttaatgcac
     2461 agtaaatgta gtctaacacg cacaatattc ctattattgt ccatgaaaaa tgtgaggtca
     2521 tatgctactg atttaaagaa atgttgaaca gcacagcaca caagaggtat cagcctctct
     2581 atatggtcca tacagcacca ggctcgctgc gttcatgtgc attcagaggc gcgcacacgt
     2641 gtggagcaga cagactcgac gcagcgggct ttgcttttta ttcatggaag cccattttag
     2701 aagaagctcg ggcagactcg tcacattctt cacttcacca taggtagatc aaatcaaagt
     2761 tattggatat aacggaacaa tattcatcat attatgagat tattattaga ccgagtgcat
     2821 tagacatatt tgtattcgat cgttgaaaaa gtatatattt tattttagcc ttaaactatg
     2881 gttagcctat attaatcagc ataatctgtt atttcagacg ttgtattaat gattatttct
     2941 agcacagctt tgatgcatca atgcaatgtt tctacaaacc cgccttgttt aatgtcggaa
     3001 aaaaagcgca acaaatgtaa agagttggca ggcattctgt aaatatttcc aggcaaaaac
     3061 atgcacaaag gaaagaaaca ccattgtttt ggtgtcttat cttcgctgga caaatatcct
     3121 gctttgggtg ccgtaatgaa ggcagggcca gtgacacaca cacatacaca cacacacata
     3181 cacgcataca cggcttcctt ctcaagcgca gtgacttcac ttcccaaatt tagaggaaaa
     3241 aaaaccatcc ggcgaaactg tctctgtctc tccgccacat atcgggggct ggaattaatt
     3301 cagacgcgca agtttgtaca aaaaagcagg ctataacttc gtatagcata cattatacga
     3361 agttatatta agggttccgg atcctcgaga ccggtcgcca ccatggccct gtccaacaag
     3421 ttcatcggcg acgacatgaa gatgacctac cacatggacg gctgcgtgaa cggccactac
     3481 ttcaccgtga agggcgaggg cagcggcaag ccctacgagg gcacccagac ctccaccttc
     3541 aaggtgacca tggccaacgg cggccccctg gccttctcct tcgacatcct gtccaccgtg
     3601 ttcatgtacg gcaaccgctg cttcaccgcc taccccacca gcatgcccga ctacttcaag
     3661 caggccttcc ccgacggcat gtcctacgag agaaccttca cctacgagga cggcggcgtg
     3721 gccaccgcca gctgggagat cagcctgaag ggcaactgct tcgagcacaa gtccaccttc
     3781 cacggcgtga acttccccgc cgacggcccc gtgatggcca agaagaccac cggctgggac
     3841 ccctccttcg agaagatgac cgtgtgcgac ggcatcttga agggcgacgt gaccgccttc
     3901 ctgatgctgc agggcggcgg caactacaga tgccagttcc acacctccta caagaccaag
     3961 aagcccgtga ccatgccccc caaccacgtg gtggagcacc gcatcgccag aaccgacctg
     4021 gacaagggcg gcaacagcgt gcagctgacc gagcacgccg tggcccacat cacctccgtg
     4081 gtgcccttct gaagctcgag ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     4141 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     4201 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     4261 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     4321 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     4381 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     4441 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     4501 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     4561 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     4621 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     4681 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     4741 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4801 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4921 aactcatcaa tgtatcttat catgtctgga tctgacatgg taagtaagct tatccccgga
     4981 gcttgggctg caggtcgagg gacctaataa cttcgtatag catacattat acgaagttat
     5041 acccagcttt cttgtacaaa gtgggatcca ctagtgctag cgccaccatg ggtaagccta
     5101 tccctaaccc tctcctcggt ctcgattcta cgggtggtgc tggtggtatg gtgagcaagg
     5161 gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg
     5221 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5281 ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc
     5341 agttcatgta cggctccagg gccttcatca agcaccccgc cgacatcccc gactactata
     5401 agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5461 ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc
     5521 tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg
     5581 aagcatccac cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg
     5641 ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc tacaaggcca
     5701 agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc
     5761 acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg
     5821 gcggcatgga cgagctgtac aagtaaggcg cgccctcgag ctcaagcttc gaattctgca
     5881 gtctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     5941 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     6001 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     6061 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttacaa ctttattata
     6121 catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat caacttgttt
     6181 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6241 tttttttcac tgcgatatca atcgggacag ccccccccca aagcccccag ggatgtaatt
     6301 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     6361 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     6421 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     6481 gatacgggga aaaagcttta ggctgagtgc acggggacag ccccccccca aagcccccag
     6541 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     6601 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     6661 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     6721 cacctggggg gatacgggga aaaagcttta ggctgatacg tagtagatgc ggccgcaact
     6781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6901 atgtctggat cgcgatcgcg tctagattat catacctttc tcttcttttt tggaccacca
     6961 gcaccacctc ctcgagcatt aagcttgtgc cccagtttgc tagggaggtc gcagtatctg
     7021 gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt gattctttcc
     7081 agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg tttcttggat
     7141 ctatatgtgg tcttggcgtt tgcgatcaga tggctcccgc ccacgagctt cagggccatg
     7201 tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa ggcctcccag
     7261 ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc tctgatcttg
     7321 acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt cagcacgccc
     7381 ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga ctgcttgaag
     7441 aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag gaagctagta
     7501 gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat tctcatggtc
     7561 tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg atggttgtcc
     7621 acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc gctcatggtg
     7681 gatccggccc cgccggctcc gccgctagcg tctccatcct ccagcagtct caccatagct
     7741 cctgtctcag agtccaggtt tctgatgtag ttcatcacaa tgttcacatt tgtccatcct
     7801 ccagcctgca tgatctcagg gatgctcact ccagctctag ccatgtctct agcagctccc
     7861 actctagcag agtgtccaga ccaagccagg tatctctgtc cagagtcatc cttagctcca
     7921 tagatcagtc tgtgtgtagc ctcaaagatt ccctccagag cccttgtgct cagctggctt
     7981 gtagcagaag gagcagccac tccattcttt ctcactctac agaacaggta gttgttaggg
     8041 tcatcagcca ctccagacac agagatccat ctctccacca gctttgtcac tcccaggctc
     8101 agagccttct ccactccagc tgtgctcacc agtgtctttg ttcttccaat gtggatcagc
     8161 attcttcctc catctgttct gctgatgtcc ttcactctga ttctagcaat ctcagcaatt
     8221 ctcagcagtg tgttgtaagc aattcccagg aaagccaggt ttctgatgtc ctgacatctg
     8281 tcagagttct ccatcaggct tctcacctgg tcaaagtctg ttctctcaaa agccagagcc
     8341 tgcttagctc tctctccagc atccacattc tcctttctga ttcttctcat caccaggctc
     8401 acagcattgc tgtcagaagg tctaggcagt ccagaccttc tgtgcagcat gttcagctgt
     8461 cccaggtgct gctggattgt cttcacagcc agtcctctag cctgtggaga gaaaggcaaa
     8521 gtggatgtca gtaagaccaa taggtgccta tcagaaacgc aagagtcttc tctgtctcga
     8581 caagcccagt ttctattggt ctccttaaac ctgtcttgta accttgatac ttacctggag
     8641 gtacagcagg tagtctctca catcctcagg ctcagcaggg aaccactttc tgttgttcag
     8701 cttacaccaa gcagcccagc tcctgcagac agacagcagc atcttccatg tgtgctcaga
     8761 gaaagcctgt ctgtctctga acatgtccat caggttcttt ctcacctcat cagatgtagc
     8821 atccacaggc agagcaggta gattttggtg gacggttaga agattggaga ctttcctctt
     8881 cttcttgggc accatggtgg ccatatgcgg cacgcgtcag gaaaaaaaaa aacaattaga
     8941 attaatttta tatttataca atttatcggt gcaattgttt cattatgaaa ggatttttct
     9001 agtcaacaag tgaaattctc ttaataacca agttattatt agttgtacaa gtaaaacgct
     9061 aattttcttt tgtagacaac attaacacaa attcatttag gacatttgct agtatttatg
     9121 gttattatgc tcatataaaa taatgtcagc aacataaagt aaatgcccgt ctgttcattg
     9181 ctttttttaa aatccttatt gtgaaaacgg gaaatataat tttctagtag acctaaaata
     9241 agatgaacat aggcagtcca catgtaaaaa gattacatat acaaagttga ttttttgaca
     9301 ttaagtacat ataatctcta tataattctg attagccaat agagtacaat atattgagac
     9361 ttaccttaca gtagattctt aaatagagcg aataaacacc tcgtcgggga aaagtcctgt
     9421 caggctgctc ttttcttctt tgtcaagtcg cttctcttcg gttgagtttc tctgcggtct
     9481 ctctttcgcc ctttaaatac agcagagcgc gcacccgttc agactctaca ctgttctgga
     9541 agcctccagt gaattcgacc aatcaaaaca cagcaggcag ccgccgtcac ttcttcagct
     9601 gtcattgaga ggagccgctg agaggaatgt agtggagatt tccagagttt taccgtgaaa
     9661 ttacgcgaaa cagcggtcaa aaatacaaag agatttaaaa tgtttttttt atttgagtgt
     9721 tttattgtac ttattgctga aatatgtgcg gttgtgtgat gtgttgtttt tccagttgca
     9781 atgaatgaac aaactaaaat gataaaatgt ttgaacgatt cagtaaaaaa aaatgcaata
     9841 aaaaaatctt gctgaaatgt tttacacagt agcaaaatcg ggcctgtggt gttttcagct
     9901 acatagaagg caaacctgtt ttatataagc taatagcttt ggtcagattt gtttaacgtt
     9961 tcaacaaaac aaatggatgt ttactgtact atattgattg gttaaaacca atactttatt
    10021 ctgggattgc aggtcttaac tatttttgta gtgttaaaaa caacttgcta acttgagagg
    10081 tcatgtttac cctcagagaa ataggcacaa gtctctttgt ttaatattgg agatcatcct
    10141 ccttgttcag tcgtggtctg tatttaattt taatgtatat gattgccgta aaagtgttag
    10201 aaagtttacc ctggtgctat gaaatcaatc tgctgctttt gacccagtta gtgtgtcttt
    10261 aatctaactt aatgagaccc cagtgtggtc aacaatcaat agaaacaaat gtgttgtttt
    10321 ataactgtta actcctatta ttttttatct tctgtagcat atagacaaaa catgctcgtt
    10381 ctaatagttt aataaaatta atttgatttt tggaaataac caagcgacac ccctgaacta
    10441 gtatcgatgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
    10501 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
    10561 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
    10621 gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt
    10681 tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt
    10741 ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
    10801 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
    10861 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
    10921 aagtacagta atcaagtaaa attactcaag tactttacac ctctgagatc tgagctccag
    10981 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
    11041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    11101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    11161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    11221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    11281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    11341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    11401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    12001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    12061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    12121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    12181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    12241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    12301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    12361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    12421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    12481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    12541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    13021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    13081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    13141 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
