LOCUS       sequence-46110-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for 
            ultrafast optogenetic control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(124..157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
       61 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      121 caaataactt cgtataggat actttatacg aagttatgct agccaccatg gactatggcg
      181 gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg
      241 ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca
      301 cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt
      361 tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct
      421 atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc
      481 cctctatgct ctaccttgcc acaggacacc gggtgcagtg gctgcgctat gcaacctggc
      541 tgctcacttg tcctgtcatc cttatccacc tgagcaacct caccggcctg agcaacgact
      601 acagcaggag aaccatggga ctccttgtct cagacatcgg gtgtatcgtg tggggggcta
      661 ccagcgccat ggcaaccggc tatgttaaag tcatcttctt ttgtcttgga ttgtgctatg
      721 gcgcgaacac attttttcac gccgccaaag catatatcga gggttatcat actgtgccaa
      781 agggtcggtg ccgccaggtc gtgaccggca tggcatggct gtttttcgtg agctggggta
      841 tgttcccaat tctcttcatt ttggggcccg aaggttttgg cgtcctgagc gtctatggct
//
