LOCUS       sequence-461120-        8934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries AID* tag, empty sgRNA, and amdS marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8934)
  TITLE     C. neoformans AID2 degron system plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHM2652
FEATURES             Location/Qualifiers
     source          1..8934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1085..1216
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             1229..1276
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     misc_RNA        complement(2167..2242)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(2766..4412)
                     /codon_start=1
                     /gene="amdS"
                     /product="acetamidase from Aspergillus nidulans"
                     /label=amdS
                     /note="confers ability to process acetamide for selection 
                     in fungal cells"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     primer_bind     complement(5209..5228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5210..5228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5235..5252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5235..5251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5244..5266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5392..5847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5479..5498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5689..5710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5978..6781)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6782..6997)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7021..7040)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7140..7162
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7200..7218)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7259..7762
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7799..7903
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7904..7972,7973..8764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8122..8141)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg ccccccctcg aggggttctg gatccggagg
     1081 tagtcctaaa gatccagcca aacctccggc caaggcacaa gttgtgggat ggccaccggt
     1141 gagatcatac cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg gcccggaggc
     1201 ggcggcgttc gtgaaggggt cagggtcaga ttataaagat gacgatgaca aggattataa
     1261 agatgacgat gacaagtaat tacttatgta gtgacccttc ttacgcgtaa tatgcatcgg
     1321 atgactgttg ctacttgtgc ttcttacgtt ttgtgtctta tttgctgatg tatacctctg
     1381 cgcttcaaaa tcttcattgc cgtgatatat cattccatgt cgtttgtccg ttattggatt
     1441 cccgatagtt gtccgcgaaa tcgaatattt ctcggctgac gacgtctctt gcaacaataa
     1501 aatggccatc actgttgaaa tttgcacgag agctataact tggaataacc ttcgttgctg
     1561 ctttatttta gtgctttcaa ctatttactc attctcaggt cgccaccatc gcattgatat
     1621 cgttccttcc atccttcctt tcctttagtc ggtcacgtct tccttaatcc gtcactcctt
     1681 ttttcagtcg ttcctggaga tcaccacgaa ataatctcga catgcactga ccatctcggt
     1741 tctgtggact gtcatgctaa gagagttcat gttgtaataa tagtttgttg catggaaaga
     1801 acggggaaag aagcaagaaa cagatgtaca gaattaccga tgtctctacg catccctgac
     1861 ctcgtccttc ctaccgcaac cgcacttcca cctgctcaca cgtcctcgtt aatgccttga
     1921 atctcagcca cacacttacc tcgcctcgac gcttgtcggg ccagggcacc cccttcatca
     1981 acctgggaag agttggcggg gcaaacggag gagggatggg gcaggaagag ggggatggcg
     2041 cacgtcgagt ggatttggaa gaaaaggggt tggtcgaggt ggaagtcgaa gcgaagatgt
     2101 tgaatgtcat gtttgggaac gggggatctt tagaagacta tgagattgaa cgagcttgat
     2161 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
     2221 ttgctatttc tagctctaaa acctaggcat gaggatatct cgcatctagc aacagtatac
     2281 cctgccggtg tatgtgcaat ttccactttg gcggataacg gaagcaaata agacggcggt
     2341 ccacgccgtc tcagtgtttt tttatttttt tcccgtcatc cattgttttt ctcctcacct
     2401 cttcattctc aacaaatggt tataattatc ggtcacacct gcaggttacc attgtccttg
     2461 cacgtatact ttgcatgcat gccgatcaat ttaaaaaata aatgaactgt aataatcatg
     2521 ctttgttttt agttctaatg caagggggat ccactagttg ccatgaagat cctgaggatt
     2581 caacggcgtt gctgagggtg ggaaggatgg tattgttgtc tgtagataga ttaatgggca
     2641 tataatgtca aaaaagatga ttgacacgat gtctgtctcg aattcgcagt gtgctaaaat
     2701 tgtatcactt ctctaatatg tatatataca ccctctaagg aaaattaacc ccttaccgcc
     2761 ttcacctatg gagtcaccac atttcccagc aacttcccca cttcctctgc aatcgccaac
     2821 gtcctctctt cactgagtct ccgtccgata acctgcactg caaccggtgc cccatggtac
     2881 gcctccggat catactcttc ctgcacgagg gcatcaagct cactaaccgc cttgaaactc
     2941 tcattcttct tatcgatgtt cttatccgca aaggtaaccg gaacaaccac gctcgtgaaa
     3001 tccagcaggt tgatcacaga ggcataccca tagtaccgga actggtcatg ccgtaccgca
     3061 gcggtaggcg taatcggcgc gatgatggcg tccagttcct tcccggcctt ttcttcagcc
     3121 tcccgccatt tctcaaggta ctccatctgg taattccact tctggagatg cgtgtcccag
     3181 agctcgttca tgttaacagc tttgatgttc gggttcagta ggtctttgat atttggaatc
     3241 gccggctcgc cggatgcact gatatcgcgc attacgtcgg cgctgccgtc agccgcgtag
     3301 atatgggaga tgagatcgtg gccgaaatcg tgcttgtatg gcgtccacgg ggtcacggtg
     3361 tgaccggctt tggcgagtgc ggcgacggtg gtttccacgc cgcgcaggat aggagggtgt
     3421 ggaaggacat tgccgtcgaa gttgtagtag ccgatattga gcccgccgtt cttgatcttg
     3481 gaggcaataa tgtccgactc ggactggcgc cagggcatgg ggatgacctt ggagtcgtat
     3541 ttccatggct cctgaccgag gacggatttg gtgaagaggc ggaggtcctc aacagagtgc
     3601 gtaatcggcc cgacaacgct gtgcaccgtc tcctgaccct ccatgctgtt cgccatcttt
     3661 gcatacggca gccgcccatg actcggcctt agaccgtaca ggaagttgaa cgcggccggc
     3721 actcgaatcg agccaccgat atccgttcct acaccgatga cgccaccacg aatcccaacg
     3781 atcgcaccct caccaccaga actgccgccg cacgaccagt tcttgttgcg tgggttgacg
     3841 gtgcgcccga tgatgttgtt gactgtctcg cagaccatca gggtctgcgg gacagaggtc
     3901 ttgacgtaga agacggcacc ggctttgcgg agcatggttg tcagaaccga gtccccttcg
     3961 tcgtacttgt ttagccatga gatgtagccc attgatgttt cgtagccctt gactcgaagc
     4021 tggtctttga gagagatggg gaggccatgg agtggaccaa cgggtctctt gtgctttgcg
     4081 tagtattcat cgagttccct tgcctgcgcg agagcggcgt cagggaagaa ctcgtgggcg
     4141 cagtttgtta actgctgggc gattgctgcc cgtttacaga atgctagcgt aacttccacc
     4201 gaggtcaact ctccggccgc cagcttggac acaagatctg cagcggaggc ctctgtgatc
     4261 ttcagttcgg cctctgaaag gatccccgat ttctttggga aatcaataac gctgtcttcc
     4321 gcaggcagcg tctggacttt ccattcatca gggatggttt ttgcgaggcg ggcgcgctta
     4381 tcagcggcca gttcttccca ggattgaggc atagacatgt tgggcgagtt tactaatgga
     4441 aaaagacaat tagcaaagag gctgcatatt aggagacgcg gtgaagattg gggaggacaa
     4501 tagggtccgt gcaccaaaaa gtcctgacgg cagctctgtt caacgcacat ccatctcttc
     4561 cagctatgcg tgtggcacca gcacggatgg attgttgcac caaaaggaca aggcgagatg
     4621 gatgcaggtc gaggcgatgc ggtgctatcg gccctcgttg tcgaacgcca cgtcgtcctc
     4681 tcggcgaccg tcaatcctgg aaggaaatga ccaagcgtgc acccagcatc cagcatccaa
     4741 catccagcac ccagcaccta gcacccatga tccaatggcc ggcatgcaga tcagcgtggg
     4801 gagagcacca tctagcagcc cctggcaggc ccagcattgt gctcggatgt gcgataggcg
     4861 gtgggtgcga taaggtggat tagtggagga tggaggactg agtggcgggc aagggaaggg
     4921 ggcgggaaga tgactcacct tgggcagtgg gtgggggatg cggtataggg gatgtatgtg
     4981 gatgctggcg gaggatagaa gctgtaagct gagctggtgc caacggtgac gctgtgagag
     5041 tggtctcggc gaccctgacg gcgactcggc gttaccccgt gtggcacacc ggacgcgcgt
     5101 cgagtgacgt tgctgctatt gttgttacca tcatcctctc ctccgacaac catacactca
     5161 gtgcacacca tgccactcga atcctgcatg cggtggagct ccaattcgcc ctatagtgag
     5221 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5281 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5341 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     5401 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5461 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5521 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5581 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5641 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5701 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5761 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5821 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     5881 cggtatttca caccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg
     5941 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc
     6001 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc
     6061 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat
     6121 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg
     6181 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc
     6241 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag
     6301 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg
     6361 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg
     6421 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga
     6481 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg
     6541 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat
     6601 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg
     6661 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata
     6721 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca
     6781 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc
     6841 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
     6901 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa
     6961 atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag gtggtatggt gcactctcag
     7021 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     7081 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     7141 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     7201 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgga
     7261 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
     7321 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
     7381 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
     7441 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
     7501 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
     7561 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
     7621 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
     7681 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
     7741 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg
     7801 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7861 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7921 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7981 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8041 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8101 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8161 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8221 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8281 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8341 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8401 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8461 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8521 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8581 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8641 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8701 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8761 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8821 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8881 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
