LOCUS       sequence-461124-       10295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan-neuronal expression of 'LITE-1' using a genomic fragment, 
            regulated by rab-3 promoter and unc-54 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS3-rab-3P::AI::lite-1G
FEATURES             Location/Qualifiers
     source          1..10295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5138..5147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7757..7779)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7879..7898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7976..7994)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8062..8166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8167..8235,8236..9027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8385..8404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9198..9786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9687..9706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9940..9957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10074..10095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10110..10115,10116..10133,10134..10140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10148..10164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10153..10175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10172..10188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10172..10188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gctaacaact tggaaatgaa ataagcttgc atgcctgcag gcgagttttg actggctttc
       61 aaaaactgag tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag
      121 acatttttgc aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct
      181 ataatagttt ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt
      241 gaaagtatgc agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata
      301 taatctaggt taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc
      361 tttcaaatgg tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc
      421 ggatttattc caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt
      481 gaaaaaatga gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg
      541 agggttccgt gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag
      601 ccacactgtt ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag
      661 atgctttcat tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa
      721 ttatgcatga gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg
      781 tgtggtttaa tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt
      841 gtatatacga gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt
      901 cttatttttt ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa
      961 aatttcattc ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag
     1021 ggcttcacag taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa
     1081 agaaatgtca gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct
     1141 tctcgttttc ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc
     1201 tttttttaag tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg
     1261 ctgcacaatt tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca
     1321 aatccggtct tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga
     1381 cgatgtgttg gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt
     1441 tggtgactaa acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag
     1501 accacgaata atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa
     1561 atctttatta gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg
     1621 taacgaaatt aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag
     1681 atagtatatt tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt
     1741 tacagttttc caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg
     1801 aagtattttt ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag
     1861 atatgagccg ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca
     1921 cttacatgga aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta
     1981 aatttttatt aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc
     2041 ctcaaaaatt gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc
     2101 aaaaaaaacc gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa
     2161 atattggcag tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc
     2221 gtgttataat aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg
     2281 acgagaaaga acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg
     2341 caatttcagc ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat
     2401 cgggaacgga cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa
     2461 ctgattttct tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc
     2521 gacgacttct tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct
     2581 gaaagataaa gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga
     2641 ttatatgact tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa
     2701 actcaatacg tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca
     2761 caatagtact tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt
     2821 tttgcgttct catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt
     2881 cccggtagga ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt
     2941 ccgtagatct tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga
     3001 gtcgtcgttg agtatcccat cattaactag tcaatctttc tctctttttc atttccccca
     3061 acttttctgc atattttttg acgacgacga cctcgacggc aacgagaata catttatcta
     3121 ttttttagta tagcatatat acattttcta cttttctcta acccatttca gacgtataga
     3181 ttttggtttt cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac
     3241 tgtctatttg atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc
     3301 ctacataaat atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt
     3361 tgcgatatta ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat
     3421 taagttggct tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt
     3481 taaaacttta ttattgacta tttatatata ttatatatta tatattttca tggtttaatc
     3541 aaaatctatt aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca
     3601 acaaaagcat ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa
     3661 atctagaaaa aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa
     3721 gcgcctacac gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc
     3781 atacactcct cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc
     3841 ggttgtgatg tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc
     3901 catcgatcca tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca
     3961 ctgagctcca agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg
     4021 tcttcttctt ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt
     4081 cctccagaga aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat
     4141 gataaaaatt tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt
     4201 gggaaattaa aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca
     4261 gacatttctt atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa
     4321 gatatctcgc tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat
     4381 ctaaatgctc ttttaaaata aatctacagt agccctattt tcagggatcc ccgggattgg
     4441 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     4501 gacccttgga gggtaccgag ctcagaaaaa atgatcgcaa aaatcctgtc gagtcgtaac
     4561 aaatgggcga tttgcgatcg aacgctctac ccaatttact atcttctgct cattcttggt
     4621 ctaaatcaat caatccgccc gaacaattct ctgctgttcc gtatttacag ctggttggtc
     4681 ttttgtctcc tagtgagttt gagttcattt tcaacaatag gaaattaatg tggaaattta
     4741 acaattcagc tcttcacgac tctccggaaa ttcaatcaag ttggcgtgcg cccgaacgga
     4801 acccgcgaaa accttcagga gttctttgca aacccgagat ctatgatcac tctttgcaat
     4861 gcattgataa tgttgaggtg accataaaaa tgtaatcccc ttttttgtaa ctgaacattt
     4921 tcagcggatt gctggcttca ttgcaactat acacgcttgg ggcaaaacgt ttgaaaccgt
     4981 tgaaggtgac aacgaagata aaacagaagt gtaatttatt gaatcaatat ttagattttg
     5041 tgccagtttt cgttgaatgt tcgtaccaaa caagccgagc gtcgtcaatt tatgatcaac
     5101 acttttttgg ctgttttcag tggacttttg gctcttacca tggcggccac ctacgccagt
     5161 aagttaatgt ttccattaat gaatttgtaa ttttgaatgt taaagtaata taggtaacaa
     5221 tacaacgctt gaaattaatt tattcctaaa aatggaatat gtattttact acatgaactg
     5281 aaaaaatagc attaccaagc ctgaaatata tggaactttc cggagggcat gagaagaatg
     5341 gggcgaagac caaacatgcc tagcgctaat attttcggga cgatgttcaa actttcgaaa
     5401 actctttggc accggaaacc aagttgtaaa tcgtttggca ttgctaaaac atacattgtt
     5461 ttgaacttta aatttaaagt gtttataaga aaacatagta ttttagtaac cgaagactgt
     5521 gaccgtcttt caggtgttct gaatgcgttt tttgaaccga aaattttttg aaactaatac
     5581 tggcaacgac caaatttggc gaaaaaaatt ttcagttgca aaagtactcg aattccaaac
     5641 tcttgagcaa ccagcaattg tcaaagttgc caaaagataa aatatttgac aaccagcaac
     5701 tgagtactaa cttactaagt tgccggttgc cttagaacat tgacgcacat cttaacatct
     5761 tacaaactaa actaactttg taaactcgga attattgtac ctgatactgt gagaaataat
     5821 cgggttatct gattttctat tgtatatttt attccagtgt caaaatgggg atacatcttg
     5881 tacattgttg gaactccaaa cttggacact gaaaccattt tctgtgttct tctggattct
     5941 tatgcggtat tcaacaatag tttttattat tacgtaatcc acatcctatt tagctcttcg
     6001 tctcccgtgc cgcaatttcc gccttggcaa tcttgttcta ccaacactgt tccgtcatcc
     6061 gtcgttcgat caagcatctg atcaatgaaa tggtacctgc cgagcaagat gagtgcccgc
     6121 ttcctgaatc gtcgttacaa aaaattcacg attgtcaaat cagttatcaa agaattttta
     6181 acggaaaagc tgtaattgaa gagtactact cgtttgtttt gttctactca tatggtgttt
     6241 gcattccaat tttctgtttc ttgatgtttg tcggaatgag tgcccagtcg gttagttttc
     6301 aataataaat tacggtcaaa tcatattagt ttagatttgc tggtccgaag ttgtttccat
     6361 tgtgatttgg attgtcaatg ccattcttgt cctcttgctg ttcagcttac cagcattcat
     6421 gatcaatgaa gatgtaagtt catttattgt cttactgatt tttcaaaaca aataatttcc
     6481 agggagatcg tcttgttgct tcgtccttcc gaatgtatca cgaaactttc catgaggaaa
     6541 gagatttgac cgttttggta gtaatgattg gagtgtaatt taaaaactgt aaatgtgtaa
     6601 tattgcagtc tcaaatgaca ttcttcacct tccaaattca ctcgaccaaa ctgacattat
     6661 ccgcttgcaa ctacttctac atggatcgct ccattcttct ctcggtaatc cgattataaa
     6721 aagaacacaa aacaatcgcc ccatgttttc agctcttctc ggcaatcctc acctacttcc
     6781 tcatcctctg ggaatttgat atcaagaaca accagagtct ccagaatatc gcaaaccaca
     6841 cgattcacac ataagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     6901 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     6961 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     7021 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     7081 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     7141 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     7201 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     7261 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     7321 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     7381 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     7441 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     7501 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     7561 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     7621 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     7681 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     7741 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7801 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7861 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     7921 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     7981 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     8041 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8161 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8221 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8281 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8341 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8401 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8461 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8521 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8581 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8641 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8701 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8761 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8821 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8881 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8941 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9001 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9061 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9121 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9181 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9241 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9301 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     9361 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9421 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9481 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9541 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9601 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9661 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9721 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9781 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9841 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9901 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9961 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10021 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10081 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10141 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    10201 aagctgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagagct
    10261 taaaaatggc tgaaatcact cacaacgatg gatac
//
